Ultrastructural Localization of Surface Receptors for Concanavalin a on Rabbit Spermatozoa

Gordon, Mildred; Dandekar, Pramila V.; Bartoszewicz, W

doi:10.1530/jrf.0.0360211

Cited by 58 publications

(20 citation statements)

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“…Although there have been several claims that the negative charge of spermatozoa decreases during capacitation or in the female tract (Vaidya et al 1971;Rosado et al 1973;Gordon et al 1974) these claims could not be substantiated in the present study. Treatment of rabbit spermatozoa in hypertonic media has been reported to release sperm bound seminal plasma proteins (Brackett & Oliphant 1975) but this procedure did not significantly alter net sperm surface charge.…”

Section: Discussioncontrasting

confidence: 68%

The Net Surface Charge of Mammalian Spermatozoa as Determined by Isoelectric Focusing. Changes Following Sperm Maturation, Ejaculation, Incubation in the Female Tract, and after Enzyme Treatment

Moore

1979

Int J of Andrology

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The mean net surface charge of living mammalian spermatozoa from various regions of the male reproductive tract has been investigated by whole cell isoelectric focusing techniques. In all cases, spermatozoa released from the caput epididymidis had a significantly higher mean isoelectric point than those from the cauda region. The net charge of spermatozoa from the rat and hamster was further modified by seminal vesicular fluid but not by prostatic secretion. Whereas the isoelectric point of caudal spermatozoa from rodents increased significantly after exposure to neuraminidase or trypsin, that of spermatozoa from the caput region was altered to a lesser extent. By contrast, neuraminidase failed to influence the surface charge of ejaculated human and rabbit spermatozoa, as did the incubation of spermatozoa in the female tract or with capacitating media. Finally, a preliminary study showed that spermatozoa from some apparently infertile men have an isoelectric point consistently higher than those from fertile men.

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Section: Discussioncontrasting

confidence: 68%

The Net Surface Charge of Mammalian Spermatozoa as Determined by Isoelectric Focusing. Changes Following Sperm Maturation, Ejaculation, Incubation in the Female Tract, and after Enzyme Treatment

Moore

1979

Int J of Andrology

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“…Surface changes have been documented by using a variety of techniques including lectin binding (Gordon et al, 1974;Nicolson et al, 1977), radiolabelling (Olson and Hamilton, 1978;Russell et al, 19851, immunofluorescence (Feuchter et al, 1981), lipid probe analysis (Bearer and Friend, 19821, freeze-fracture (Friend and Fawcett, 1974;Koehler, 19821, etc. The literature pertaining to surface maturational changes is, in part, reviewed by Bellve and Moss (1983) and Myles et al (1981).…”

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confidence: 99%

Immunofluorescence antigen localization on boar sperm plasma membranes: Monoclonal antibodies reveal apparent new domains and apparent redistribution of surface antigens during sperm maturation and at ejaculation

1986

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Purified boar sperm plasma membranes (PM) and PM proteins were used as antigens to produce 58 monoclonal antibodies against surface antigens. Fluorescence labelling (biotin-avidin-FITC) was used to determine the distribution of antigens in caput and cauda epididymal and in ejaculated spermatozoa with hybridoma supernatants and/or 1:100 diluted ascites fluid after subcloning. Sixteen areas (subdomains) of apparent restricted antigen mobility were identified and significant differences in the localization of most antigens in caput, cauda, and ejaculated PM were recognized. While localization patterns were highly reproducible with a given protocol for sample preparation and immunolabelling, localization patterns were markedly affected by changes in protocols. Fluorescence patterns were affected by the manner in which sperm were labelled (live sperm or sperm labelled at various steps), by washing, and by temperature or by addition of seminal plasma. These results indicate that the dynamic properties of the sperm PM or the surrounding fluids can easily mask or unmask or reconfigure binding sites for highly site-specific monoclonal antibodies and that antigen distribution is probably under-estimated when these labelling techniques are used. Such changes in the accessibility of antigenic sites to monoclonal antibodies limited determining the extent of distribution of a given antigen on epididymal sperm. However, the reproducibility of patterns when a given protocol is used and the large number of antibodies (39/42) displaying marked differences in localization on caput, cauda, and ejaculated PM suggest that changes in the organization of the PM constituents, whether by addition or subtraction of antigen or through configurational changes in proteins, are a major consequence of sperm maturation in the epididymis.

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“…Structural modifications subjacent to the plasmalemma such as the post-acrosomal dense lamina and the posterior ring at the head-neck juncture in mammalian spermatozoa (Fawcett, 1975) do not exist in cock spermatozoa (Bakst and Howarth, 1975). Freeze-fractured preparations (Friend and Fawcett, 1974) and the visualization of anionic sites (Yanagimachi et al, 1972) and of Con A binding sites (Gordon et al, 1974) on spermatozoa from various mammalian species have shown differences in the segmental organization of membrane associated glycoprotein and glycolipid moieties. Although their full significance is not known, it is assumed that these macromolecules play a role in capacitation, gamete recognition and attachment (Fawcett, 1975;Johnson, 1975;Baccetti and Afzelius, 1976).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Glycerol and Its Removal on Cock Spermatozoa Concanavalin A and Cationized Ferritin Binding Sites

Bakst

Howarth

1977

Poultry Science

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Concanavalin A (Con A) and cationized ferritin (CF) were utilized to assess the effects of glycerol and its removal on their respective sperm plasma membrane binding sites. Diluted semen was equally divided: One part diluted with a diluent that was 15% glycerol by volume and the other was unchanged. Sperm labeling with Con A and CF was done with diluted spermatozoa, diluted spermatozoa held for 3 hr. at room temperature, glycerol-treated spermatozoa and dialyzed, glycerol-treated spermatozoa. Both probes uniformly labeled the surface of spermatozoa from all treatments. The plasma membrane of some glycerol-treated spermatozoa and of some dialyzed spermatozoa exhibited disruption in the form of ruffling, ballooning, and discontinuity. Nearly 60% of the dialyzed, glycerol-treated spermatozoa were observed to have some surface membrane irregularity. Glycerol-induced osmotic effects may have resulted in the surface damage. In addition, glycerol's ability to destabilize membranes may have rendered the sperm's plasmalemma more labile thus augmenting the osmotic-induced damage. It was concluded that the loss of fertility after vaginal insemination of glycerol-treated spermatozoa did not appear to be associated with alterations of the surface glycoproteins which bind Con A and CF. However, it may be related to the loss of plasma membrane integrity.

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Ultrastructural Localization of Surface Receptors for Concanavalin a on Rabbit Spermatozoa

Cited by 58 publications

References 0 publications

The Net Surface Charge of Mammalian Spermatozoa as Determined by Isoelectric Focusing. Changes Following Sperm Maturation, Ejaculation, Incubation in the Female Tract, and after Enzyme Treatment

The Net Surface Charge of Mammalian Spermatozoa as Determined by Isoelectric Focusing. Changes Following Sperm Maturation, Ejaculation, Incubation in the Female Tract, and after Enzyme Treatment

Immunofluorescence antigen localization on boar sperm plasma membranes: Monoclonal antibodies reveal apparent new domains and apparent redistribution of surface antigens during sperm maturation and at ejaculation

The Effect of Glycerol and Its Removal on Cock Spermatozoa Concanavalin A and Cationized Ferritin Binding Sites

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