Ultrastructural localization of transferrin synthesis in rat hepatocytes during prenatal and postnatal development

Kraemer, Michel; Vassy, Jany; Foucrier, Jean; Chalumeau, M.T.

doi:10.1016/0045-6039(81)90003-8

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…From day 12 of gestation, hepatocytes are able to synthesize and export plasma proteins, such as albumin and transferrin (26)(27)(28). Moreover, albumin messenger RNA (mRNA) and a-fetoprotein mRNA can be detected in cells of the ventral foregut 1 day and 2 days earlier, respectively (29).…”

Section: Discussionmentioning

confidence: 99%

Confocal microscopy immunofluorescence localization of desmin and other intermediate filament proteins in fetal rat livers

et al. 1993

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Immunolocalization of desmin in fetal rat livers shows that on day 12 of gestation a high number of liver cells express desmin. This number decreases from day 14 onward. On day 20 about the same density of desmin-containing cells is found in fetal rat livers as is found in adult rat livers. Desmin-containing cells show two types of labeling patterns, especially on days 12 and 13 of gestation: (a) a basketlike network of intermediate filaments throughout the whole cell, similar to the labeling pattern of cytokeratin in hepatocytes; and (b) more strongly labeled intermediate filaments developing long and slender processes between adjacent cells, close to the labeling pattern of Ito cells in adult rats. From day 14 of gestation, the first type becomes rare, and from day 18 only the second type remains. Double-labeling experiments show that coexpression of desmin and cytokeratin is found in cells of the first type on days 12, 13 and 14 of gestation. Cells containing desmin with the labeling pattern of the second type never express cytokeratin. Coexpression of vimentin and cytokeratin is never found in fetal hepatocytes, even on day 12 of gestation. Numerous nonhepatocyte cells coexpress desmin and vimentin, but some cells contain vimentin or desmin alone. In desmin-containing cells the labeling pattern is of the first type (basketlike network). These results suggest that in early stages of fetal liver development, desmin is found in two different types of liver cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Confocal microscopy immunofluorescence localization of desmin and other intermediate filament proteins in fetal rat livers

et al. 1993

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“…The differentiation of hepatocytes during gestation may be subdivided into three stages: (a) days 12-14, characterized by potato-shaped hepatocytes, scattered throughout the liver tissue (Asano et al, 1987;Vassy et al, 1988), containing no glycogen but synthesizing and exporting plasma proteins (Kraemer et al, 1981(Kraemer et al, , 1986Yeoh, 1986); at that stage, haematopoietic cells are predominant; (b) days 15-17 are characterized by a more compact liver, with hepatic cords (about five cells thick) and spherical hepatocytes; days 16 and 17 are considered to be a key period for enzyme differentiation (Yeoh, 1986) and synthesis of soluble cellular proteins (Radford & Bhathal, 1987); (c) days 18-21 are characterized by the emergence of glycogen deposits, cubic hepatocytes and hepatic cords constituting the muralium duplex (Elias & Scherrick, 1969), consisting of a set of two-cell-thick tissue sheets (Medlock & Harr, 1983;Asano et al, 1987;Vassy et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Analysis by confocal scanning laser microscopy imaging of the spatial distribution of intermediate filaments in foetal and adult rat liver cells

Vassy

Rigaut

Hill

et al. 1990

Journal of Microscopy

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SUMMARY Confocal scanning laser microscopy has been used to make three‐dimensional observations of the spatial distribution of cytoskeleton intermediate filaments in rat liver hepatocytes, at various stages during foetal development and in the adult. Single and double immuno‐labelling with fluorescein and Texas Red fluorescence have been used to study the intracellular spatial distribution of C18 cytokeratin and vimentin. Simultaneous confocal imaging with double‐fluorescence emission requires an image processing step for the correction of ‘contamination’ effects due to the overlap between fluorescein and Texas Red emission spectra. At the pre‐natal period (day 20 of gestation) each type of intermediate filament labelling is only present in a certain cellular category, C18 cytokeratin in hepatocytes and vimentin in mesenchymal cells. However, at the earliest developmental stages (day 12 of gestation), vimentin and cytokeratin seem to be found in the same type of cells, probably mesenchymal cells. Some striking developmental changes, associated with the differentiation of the liver parenchyma, are observed for both C18 cytokeratin and vimentin. In earlier foetal stages, C18 filaments are scarce, hazily labelled and randomly distributed inside the hepatocytic cytoplasm. Late during foetal development (days 18–20 of gestation), hepatocytic cytokeratin filaments are abundant, well individualized and sharply labelled. The hepatocytes are arranged in a muralium duplex architecture (two‐cell‐thick sheets) and the labelling intensity measured in the hepatocytic cytoplasm at the basal pole is double that measured at the sinusoidal pole, while, in the adult, hepatocytes are arranged in a muralium simplex architecture (one‐cell‐thick sheets) and cytokeratin filaments have a symmetrical distribution in relation to the nuclear region.

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Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation

Vassy

Irinopoulou

Beil

et al. 1997

Microsc. Res. Tech.

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Ultrastructural localization of transferrin synthesis in rat hepatocytes during prenatal and postnatal development

Cited by 7 publications

References 34 publications

Confocal microscopy immunofluorescence localization of desmin and other intermediate filament proteins in fetal rat livers

Confocal microscopy immunofluorescence localization of desmin and other intermediate filament proteins in fetal rat livers

Analysis by confocal scanning laser microscopy imaging of the spatial distribution of intermediate filaments in foetal and adult rat liver cells

Spatial distribution of cytoskeleton intermediate filaments during fetal rat hepatocyte differentiation

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