Ultrastructural morphology and protein content of the internal organic material of rat otoconia

Pote, Kenneth G.; Ross, Muriel D.

doi:10.1016/0889-1605(86)90029-7

Cited by 29 publications

(9 citation statements)

References 30 publications

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“…The 54 kDa protein present in the asteriscus and lapillus may correspond to the 54 kDa protein found in the vateritic otoconia of the gar pike (Pote & Ross, 1986. The band observed around 55 kDa may correspond to otolith matrix protein-1 (OMP-1), one of the major components of fish sagittae according to Murayama et al (2000).…”

Section: Discussionmentioning

confidence: 99%

Morphological and biochemical analyses of otoliths of the ice‐fish Chionodraco hamatus confirm a common origin with red‐blooded species

Motta¹,

Avallone²,

Balassone

et al. 2008

Journal of Anatomy

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The morphology and composition of the three otoliths of the Antarctic ice-fish Chionodraco hamatus were studied by scanning electron microscopy and X-ray diffraction. The composition of the sagitta, lapillus and asteriscus protein matrices was also analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, western blots and confocal laser scanning microscopy to reveal the presence of and to localize the calcium-binding proteins calmodulin, calbindin and S-100. Morphological results indicated that the otoliths in this ice-fish were similar to those of Trematomus bernacchii , a red-blooded Antarctic species [B. Avallone et al. (2003) J. Submicrosc. Cytol. Pathol. 35 , 69-76], but rather different from those of other teleosts. These two Antarctic species possessed a completely vateritic asteriscus, whereas their sagitta and lapillus were made mostly of aragonite. Parallel analysis of protein patterns in C. hamatus and T. bernacchii revealed that the sagitta significantly differed from the lapillus and asteriscus in both species. The sagitta did not contain the S-100 protein and showed calmodulin and calbindin located in discontinuous or incremental zones, respectively. These results demonstrate that the otoliths of C. hamatus and T. bernacchii share more resemblances than differences and support the idea of a common origin of these species.

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Section: Discussionmentioning

confidence: 99%

Morphological and biochemical analyses of otoliths of the ice‐fish Chionodraco hamatus confirm a common origin with red‐blooded species

Motta¹,

Avallone²,

Balassone

et al. 2008

Journal of Anatomy

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“…Many transmission electron microscopy studies of decalcified calcite otoconia have shown well-ordered organic material distributed throughout the otoconia (21,22). Salamat et al (5) also demonstrated that the decalcified fetal otoconia of the rat consist of rows of filaments arranged around the center and fibrous-appearing strands added to the surface.…”

Section: Discussionmentioning

confidence: 99%

Production of Otoconia in the Endolymphatic Sac in the Japanese Red-bellied Newt,Cynops pyrrhogaster: Light and Transmission Electron Microscopic Study

Gao¹

1998

Acta Oto-Laryngologica

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The formation of otoconia in the endolymphatic sac (ES) of the larval newt, Cynops pyrrhogaster, has been studied by light and transmission electron microscopy. Some of the epithelial cells of the ES contain an abundance of swollen vesicles, Golgi complexes, rough endoplasmic reticula and ribosomes at the late larval stages 50 and 51, approximately 26-30 days after eggs are laid. Five days later, at stage 52, crystals are present in the vacuoles between the epithelial cells. Serial sections indicate that these vacuoles actually form small canals which lie in the wall and join the lumen of the ES. Reconstruction of the ES shows that several canals are contained in the ES wall. At stage 56, about 72 days after eggs are laid, a large number of otoconia are present in the ES lumen, while the otoconia disappear from the canals. It appears that the otoconia are first produced in the canals and then released to the lumen. Some epithelial cells of the ES are thought to expel the organic and inorganic material to the canals to form the otoconia in situ. The process of formation of the otoconia in the ES is discussed.

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“…Furthermore, the otoconia grow until other nucleation centers appear, which produces zones without local supersaturation inducing otoconium growth inhibition (po = ps) or otoconium dissolu tion (po < ps). Recently, Pote and Ross [23] clearly demonstrated the protein content and also the presence of a core in rat oto conia.…”

Section: Discussionmentioning

confidence: 99%

Human Otoconial Crystal Growth

Sánchez-fernández

Rivera‐Pomar²,

Tello³

1989

ORL

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The otoconium genesis was evaluated by morphometric procedures using transmission and scanning electron-microscopic techniques for human fetuses of 50, 110, 134, 173, 210, 220 and 232 mm crown-rump length. Different otoconial crystalline habits were found. For the elongated otoconia (hexagonal prism) different parameters were measured: (1) the mean cleavage angle value of the terminal rhombohedron was 102 (SD 0.65), which was independent of the fetal age; (2) large and short axes show a linear growing law in function of the fetal age, and (3) the otoconial volume grows following a quadratic law. A hypothesis about the physicochemical growth conditions is postulated.

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Ultrastructural morphology and protein content of the internal organic material of rat otoconia

Cited by 29 publications

References 30 publications

Morphological and biochemical analyses of otoliths of the ice‐fish Chionodraco hamatus confirm a common origin with red‐blooded species

Morphological and biochemical analyses of otoliths of the ice‐fish Chionodraco hamatus confirm a common origin with red‐blooded species

Production of Otoconia in the Endolymphatic Sac in the Japanese Red-bellied Newt,Cynops pyrrhogaster: Light and Transmission Electron Microscopic Study

Human Otoconial Crystal Growth

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