Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration

Liang, Wei; Zhou, Yue; Wang, Lingxia; You, Xiangrong; Zhang, Yaping; Cheng, Chi‐Lien; Chen, Wei

doi:10.1016/j.jprot.2012.07.041

Cited by 34 publications

(40 citation statements)

References 49 publications

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“…Here we put forward several possibilities: first, potentially alleviating the counter-force from the EPS, as mentioned above; second, a slow growth rate may allow this species to save enough energy to push the elongation of trichomes in a straight direction; third, it may facilitate the establishment of enough space with less physical barrier for the trichome elongation by secreted enzymes or via other means. Ultra-structural observation of natural N. flagelliforme showed that the cells were located in the voids of the EPS matrix (Liang et al, 2012), which potentially supports the third possibility. Epiphytic microorganisms can give rise to the disintegration of the EPS matrix under laboratory conditions (Gao and Ye, 2003; Gao et al, 2012); however, in native complex environments, their proper hydrolysis upon short-term rewetting may also facilitate the softening of the EPS matrix, thus benefiting the release of growth space.…”

Section: Discussionmentioning

confidence: 78%

The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium Nostoc flagelliforme

Cui

Zhu

et al. 2017

Biology Open

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The terrestrial cyanobacterium Nostoc flagelliforme, which contributes to carbon and nitrogen supplies in arid and semi-arid regions, adopts a filamentous colony form. Owing to its herbal and dietary values, this species has been overexploited. Largely due to the lack of understanding on its morphogenesis, artificial cultivation has not been achieved. Additionally, it may serve as a useful model for recognizing the morphological adaptation of colonial cyanobacteria in terrestrial niches. However, it shows very slow growth in native habitats and is easily disintegrated under laboratory conditions. Thus, a novel experimental system is necessary to explore its morphogenetic mechanism. Liquid-cultured N. flagelliforme has been well developed for exopolysaccharide (EPS) production, in which microscopic colonies (micro-colonies) are generally formed. In this study, we sought to gain some insight into the morphogenesis of N. flagelliforme by examining the effects of two external factors, the EPS and environmental stress-related growth rate, on the morphological shaping of micro-colonies. Our findings indicate that the EPS matrix could act as a basal barrier, leading to the bending of trichomes during their elongation, while very slow growth is conducive to their straight elongation. These findings will guide future cultivation and application of this cyanobacterium for ecological improvement.

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Section: Discussionmentioning

confidence: 78%

The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium Nostoc flagelliforme

Cui

Zhu

et al. 2017

Biology Open

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“…Two-dimensional gel electrophoresis was undertaken according to the method of Liang et al [84] and Liu et al [85] with the Ettan IPGphor system (GE Healthcare, Little Chalfont, UK). The leaf protein (1,500 µg) was loaded onto IPG strips (24 cm, linear gradient pH 4–7; GE Healthcare) in a rehydration tray for 12 h. Isoelectric focusing (IEF) was performed under the following conditions: 200 V for 1 h, 500 V for 1 h, 1 kV for 1 h, gradient to 8 kV for 0.5 h, and, finally, 8 kV for a total of 48,000 Vhs at 20°C.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Analysis of Salt-Responsive Proteins in the Leaves of Mangrove Kandelia candel during Short-Term Stress

et al. 2014

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Salt stress is a major abiotic stress that limits crop productivity in many regions of the world. A comparative proteomic approach to identify salt stress-responsive proteins and to understand the molecular mechanisms was carried out in the woody halophyte Kandelia candel. Four-leaf-old K. candel seedlings were exposed to 150 (control), 300, 450, and 600 mM NaCl for 3 days. Proteins extracted from the leaves of K. candel seedlings were separated by two-dimensional gel electrophoresis (2-DE). More than 900 protein spots were detected on each gel, and 53 differentially expressed protein spots were located with at least two-fold differences in abundance on 2-DE maps, of which 48 were identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF-TOF/MS). The results showed that K. candel could withstand up to 450 mM NaCl stress by up-regulating proteins that are mainly involved in photosynthesis, respiration and energy metabolism, Na+ compartmentalization, protein folding and assembly, and signal transduction. Physiological data, including superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities, hydrogen peroxide (H2O2) and superoxide anion radicals (O2 −) contents, as well as Na+ content and K+/Na+ ratios all correlated well with our proteomic results. This study provides new global insights into woody halophyte salt stress responses. Identification of differentially expressed proteins promotes better understanding of the molecular basis for salt stress reduction in K. candel.

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“…It is extremely harsh niches let N. flagelliforme evolve abilities for desiccation tolerance and UV insensitivity (Gao and Ye, 2007;Ferroni et al, 2010;Shang et al, 2018). During the last 30 years, studies have focused on specific structural, physiological and proteomic aspects of desiccation-tolerant cyanobacteria (Potts, 1999;Qiu et al, 2003;Gao and Ye, 2007;Zhao et al, 2008;Liang et al, 2012;Liu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

Shang

Chen

Hou

et al. 2019

Environmental Microbiology

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Summary The cyanobacterium Nostoc flagelliforme is an extremophile that thrives under extraordinary desiccation and ultraviolet (UV) radiation conditions. To investigate its survival strategies, we performed whole‐genome sequencing of N. flagelliforme CCNUN1 and transcriptional profiling of its field populations upon rehydration in BG11 medium. The genome of N. flagelliforme is 10.23 Mb in size and contains 10 825 predicted protein‐encoding genes, making it one of the largest complete genomes of cyanobacteria reported to date. Comparative genomics analysis among 20 cyanobacterial strains revealed that genes related to DNA replication, recombination and repair had disproportionately high contributions to the genome expansion. The ability of N. flagelliforme to thrive under extreme abiotic stresses is supported by the acquisition of genes involved in the protection of photosynthetic apparatus, the formation of monounsaturated fatty acids, responses to UV radiation, and a peculiar role of ornithine metabolism. Transcriptome analysis revealed a distinct acclimation strategy to rehydration, including the strong constitutive expression of genes encoding photosystem I assembly factors and the involvement of post‐transcriptional control mechanisms of photosynthetic resuscitation. Our results provide insights into the adaptive mechanisms of subaerial cyanobacteria in their harsh habitats and have important implications to understand the evolutionary transition of cyanobacteria from aquatic environments to terrestrial ecosystems.

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Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration

Cited by 34 publications

References 49 publications

The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium Nostoc flagelliforme

The effects of the exopolysaccharide and growth rate on the morphogenesis of the terrestrial filamentous cyanobacterium Nostoc flagelliforme

Proteomic Analysis of Salt-Responsive Proteins in the Leaves of Mangrove Kandelia candel during Short-Term Stress

Genomic and transcriptomic insights into the survival of the subaerial cyanobacterium Nostoc flagelliforme in arid and exposed habitats

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