Ultrastructural studies of three species of Oesophagostomum (nematoda) by scanning electron microscopy

Gaddam, Rajeshwari; Murthy, G. S. Sreenivasa; Sudhakar, K.

doi:10.1007/s12639-017-0897-3

Cited by 2 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Males also have a shovel-shaped gubernaculum, as well as a genital cone containing a pair of spherical cuticular appendages. These results confirm the data obtained by most authors who studied the morphological structure of nematodes of this species using SEM (Ivashkin et al, 1998;Lapage, 2000;Khanmohammadi et al, 2013;Gaddam et al, 2017aGaddam et al, , 2017b. We also described in more detail the morphological features of the distal and proximal ends of the spicules.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat

Prijma,

Ohorodnyk,

Krykunova

et al. 2023

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

The nematodes of the genus Oesophagostomum Molin, 1861 parasitize in the large intestine of ruminants and are widely represented in the terrestrial ecosystems of many countries of the world. One of the most common species of this genus in small ruminants is Oesophagostomum venulosum Rudolphi, 1809. The validity of this species was established using morphological and molecular genetic methods, the importance of which is still relevant. The aim of our research was to determine the morphological and metric characteristics of O. venulosum nematodes isolated from domestic goats in Ukraine. The differential parameters of mature males and females of nematodes of this species are described and illustrated, and the obtained data is analyzed in the context of the findings of other authors. The taxonomic morphological characters of O. venulosum include the presence of a pronounced cuticular vesicle at the head end, inner and external corona radiata, cervical papillae located behind the esophagus; in males, the presence of prebursal papillae in front of the tail bursa and features of its structure, thin and tubular spicules and features of the structure of their proximal and distal ends; in females, features of the structure of the tail end, vulva and its location. 35 morphometric indicators were determined and proposed to be used in the identification of O. venulosum in males, and 29 indicators in females. Of these, 20 parameters characterize the general body structure (the length and width of the nematode, the dimensions of the mouth capsule, cervical vesicle, esophagus, the length of the inner and outer radial crowns, the location of the nerve ring and cervical papillae, as well as their sizes). In male nematodes of this species, 15 indicators characterize the metric parameters of the reproductive system (the size of the spicules in their various parts, gubernaculum, genital cone, genital papillae and their location, the width of the copulatory bursa). In females, 7 indicators are described that characterize the length of the vagina, the ovipositor, the location of the vulva and anus, the width of the body in the area of the vulva and anus, as well as two metric parameters of eggs. The identified and described morphometric characters of O. venulosum males and females based on microscopic studies can be an economical and effective method for researchers to accurately identify nematodes of this species. The information obtained in this study can contribute to the timely planning of control and prevention strategies for the parasitism of these nematodes on goat farms.

show abstract

Section: Discussionsupporting

confidence: 91%

“…Most researchers testify to such taxonomic characters (Ivashkin et al, 1998;Gaddam et al, 2017a;Amuzie et al, 2022). At the same time, some authors note the impossibility of studying the vulva region due to the presence of genital cement in the tail part (Khanmohammadi et al, 2013;Gaddam et al, 2017b).…”

Section: Discussionmentioning

confidence: 99%

Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat

Prijma,

Ohorodnyk,

Krykunova

et al. 2023

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

show abstract

“…Considering only the species with the greatest impact on public health and veterinary medicine, pigs can be infected with the species O. dentatum and O. quadrispinulatum (Lin et al, 2014). Oesophagostomum bifurcum, O. stephanostomum, O. brumpti and O. aculeatum have previously been described in human and non-human primates (Glen & Brooks, 1985); and O. columbianum, O. venulosum and O. asperum in sheep and goats (Gaddam et al, 2017). Despite the first human case of 'O.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome c oxidase subunit 1 gene reveals species composition and phylogenetic relationships of Oesophagostomum spp. infecting pigs in northeastern Brazil

Bacelar

Monteiro

Calegar

et al. 2022

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Helminths of the genus Oesophagostomum cause enteric diseases and affect domestic animals such as pigs. The aim of this study was to explore the species composition and genetic diversity of Oesophagostomum spp. infecting pigs in close contact with humans in the state of Piauí, Brazil. Eighty-seven fecal samples were collected for parasitological tests and molecular analysis. Through microscopy, the overall positivity rate for strongyliform eggs was 81.6% among the pigs studied. Forty-two strongyliform egg samples were subjected to PCR and six cox1 sequences (637 bp) were identified for the genus Oesophagostomum. The sequences were identified as Oesophagostomum dentatum, O. quadrispinulatum and O. columbianum. In the phylogenetic tree and haplotype network, 89 sequences were separated into seven clusters, which also included reference sequences from GenBank. Oesophagostomum dentatum and O. quadrispinulatum were seen to be closely related species and formed a monophyletic group related to O. aculeatum. Oesophagostomum columbianum showed similarity with sequences from parasites infecting small ruminants and the clade was positioned closer to O. bifurcum. High interspecific diversity was found and intraspecific diversity varied according to the species. This was the first study to characterize Oesophagostomum DNA sequences obtained from pigs in Brazil.

show abstract

Ultrastructural studies of three species of Oesophagostomum (nematoda) by scanning electron microscopy

Cited by 2 publications

References 3 publications

Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat

Morphometric characteristics of the nematode Oesophagostomum venulosum (Nematoda, Strongylida) isolated from the domestic goat

Cytochrome c oxidase subunit 1 gene reveals species composition and phylogenetic relationships of Oesophagostomum spp. infecting pigs in northeastern Brazil

Contact Info

Product

Resources

About