Ultrastructural studies on the cells in vivo, in vitro of ameloblastoma.

We investigated the behaviour of human ameloblastoma cells in collagen matrix in vitro. The ameloblastoma tissue was extirpated from a 75 yr-old woman. Small segments of the tissues were cultured in 0.18% collagen gel. After several weeks, collagen gels containing ameloblastoma tissue were fixed and examined by light microscopy and transmission electron microscopy. Outgrowth of primary cultured cells could be recognized as processes like growth rims around the tissue pieces within 2-3 days after tissue culture. Light microscopically, these cells formed a duct-like structure. The characteristics of ameloblastoma cells in vivo with peripheral epithelial cells and stellate cells reappeared in vitro, and they were connected with one another in cytoplasmic processes by desmosomes. Also, some cells had many intracellular filaments, amorphous nuclei and vacuoles. The results indicated that this culture system in vitro might be applied to the cytogenetic analysis of ameloblastoma.

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Behaviour of human ameloblastoma cells in collagen matrix in vitro: an ultrastructural study

Yasuda

Satomura

Nagayama

1991

J Oral Pathology Medicine

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The behavior of human ameloblastoma tissue in collagen matrix in vitro: an immunohistochemical study

Yasuda¹,

Nakanishi²,

Satomura³

et al. 1991

J Oral Pathology Medicine

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We investigated the three-dimensional outgrowth of human ameloblastoma cells cultured in collagen matrix. Growth patterns of four cases of ameloblastoma resembled each other and was characterized by appeared duct-like structures. Case 1 ameloblastoma was subcultured 3 times. Ameloblastoma tissues and cultured cells were stained with various lectins by avidin-biotin peroxidase complex (ABC) staining methods. Both the tissues and cultured cells had the same receptors to Concanavalin ensiforme (Con A), Ricinus communis Agglutinin (RCA-I) and Triticum vulgaris (WGA) but not to Dolichos biflorus (DBA), Ulex europaeus (UEA-1), Soybean (SBA) and Arachis hypogea (PNA). These findings indicated that the cultured cells examined in this study were ameloblastoma cells in origin.

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Ultrastructural studies on the cells in vivo, in vitro of ameloblastoma.

Cited by 2 publications

References 13 publications

Behaviour of human ameloblastoma cells in collagen matrix in vitro: an ultrastructural study

Behaviour of human ameloblastoma cells in collagen matrix in vitro: an ultrastructural study

The behavior of human ameloblastoma tissue in collagen matrix in vitro: an immunohistochemical study

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