Ultrastructural study of human papilloma virus infection in the uterine cervix.

Kera, Kota; Horiuchi, Fumio; Takeda, Bin; Takamizawa, H

doi:10.5795/jjscc.26.551

Cited by 3 publications

(3 citation statements)

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“…Another study has reported the presence of HPV particles in the nuclear membrane in the vicinity of irregularly condensed chromatin in condyloma patients. (Casas-Cordero et al, 1981;Kera et al, 1987). Although HSV was not detected in this study, high-risk HPV was detected in all CIN1+ cases.…”

Section: Discussioncontrasting

confidence: 43%

A Comparison of Cytomorphological Features of ASC-H Cells Based on Histopathological Results Obtained from a Colposcopic Target Biopsy Immediately after Pap smear Sampling

Hata

Okayama

Iijima

et al. 2019

Asian Pac J Cancer Prev

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Background: To compare the cytomorphological features of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) observed in a liquid-based Pap smear with the histopathological features observed in a concurrent colposcopic biopsy specimen obtained immediately after obtaining the Pap smear. Methods: Cytomorphological features such as cytoplasmic differentiation, nuclear/cytoplasm (N/C) ratio, chromatin pattern, thickening of nuclear contour, and the appearance of the nucleolus of 247 ASC-H obtained from 25 liquid-based Pap smear ASC-H cases were compared with those of the cells obtained from biopsied samples. Human papillomavirus (HPV) infection was tested for 39 HPV genotypes using Uniplex E6/E7 polymerase chain reaction method. Results: Of the 25 ASC-H cases, 22 (88%) showed cervical intraepithelial neoplasia grade 1 or greater (CIN1+) and 3 (12%) were benign. HPV infection was detected in 100% CIN1+ cases and 66.7% benign cases. Significant differences such as marked hyperchromasia, thickened nuclear contour, and prominent nucleoli were observed between ASC-H cases with CIN1+ and the benign cases. Conclusion: The presence of small dysplastic cells displaying marked hyperchromasia, thickening of nuclear contour, and prominent nucleoli on Pap smear strongly suggest the presence of CIN in ASC-H cases.

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Section: Discussioncontrasting

confidence: 43%

A Comparison of Cytomorphological Features of ASC-H Cells Based on Histopathological Results Obtained from a Colposcopic Target Biopsy Immediately after Pap smear Sampling

Hata

Okayama

Iijima

et al. 2019

Asian Pac J Cancer Prev

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“…It is possible that HPV infection causes nuclear contour thickening in squamous cells by a similar mechanism. An ultrastructural study of HPV condyloma cases used transmission electron microscopy to demonstrate that HPV particles exist in association with condensed chromatin in the nuclear margin . In addition, Tachibana et al reported increased chromatin and partial nuclear contour thickening in ASC‐US cases with high‐risk HPV infection.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, however, nuclear enlargement without thickened nuclear contour was also observed in many high‐risk HPV‐positive cases. Ultrastructural analysis by Kera et al demonstrated that HPV particles in the nucleus were widely localised not only to the nuclear margins but also to the nuclear chromatin and nucleoli, suggesting that nuclear contour thickening in the enlarged nucleus is attributable to HPV reproduction and genotype.…”

Section: Discussionmentioning

confidence: 99%

A study of the relationship between nuclear contour thickening, nuclear enlargement and human papillomavirus infection in squamous cells

et al. 2019

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Objective To investigate the relationship between the morphological features of nuclear enlarged cells and human papillomavirus (HPV) infection in negative for intraepithelial lesion or malignancy (NILM) and atypical squamous cells of undetermined significance (ASC‐US). Methods In total, 128 Papanicolaou specimens comprising 41 ASC‐US cases and 87 NILM cases were examined. Cell morphological analysis was performed using both area ratio (nuclear area in cells with nuclear enlargement/nuclear area in normal intermediate cells) and nuclear contour thickening. High‐risk HPV was detected using the Uniplex E6/E7 polymerase chain reaction assay and logistic regression analyses of factors related to high‐risk HPV infection were performed. Results Nuclear contour thickening was present in 57.7% (64/111 cells) of high‐risk HPV positive cases and 21.8% (69/317 cells) of high‐risk HPV negative cases. There was a statistically significant association (P = 0.01) between high‐risk HPV infection and nuclear contour thickening. Nuclear contour thickening was approximately one‐third higher in NILM cases than in ASC‐US cases (odds ratio, 0.371; 95% confidence interval, 0.208–0.662) and three times higher in high‐risk HPV‐positive cases than in high‐risk HPV‐negative cases (odds ratio, 2.831; 95% confidence interval, 1.591–5.039). Conclusion Our results suggest that nuclear contour thickening in nuclear enlarged cells in NILM and ASC‐US cases may be a cellular finding associated with HPV infection.

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