Ultrastructural study of penetration of maize leaves by Colletotrichum graminicola

Politis, D. J.; Wheeler, Harry

doi:10.1016/0048-4059(73)90056-8

Cited by 75 publications

(47 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(14,37). In the same way, the lipid globules observed in appressoria were similar to those demonstrated in the appressoria of C. graminicola germinated on the leaves of maize (30). A decreasing conidial lipid content, while the germinating process moves forward, is in agreement with studies which described lipid processing during the germination of different fungi (7,19,20).…”

Section: Discussionsupporting

confidence: 80%

“…Previous studies demonstrated that the lipid content decreases during aging with concomitant cytoplasmic vacuolation (16,23). Large lipid globules were also shown in the appressoria of C. graminicola germinated on maize and oat leaves (29,30). Lipid bodies digestion inside of the fungal vacuoles was suggested (39).…”

mentioning

confidence: 98%

“…This enzyme generates orthophosphate as a result of hydrolysis of the organic phosphate (8) and, in different organisms, has been used as a marker for lysosomal structures (9,33). Vacuolation of the appressoria was observed during fungal germination (22, 28), including that of C. graminicola germinated on maize leaves (30). In the present investigation wedemonstrate the acid phosphatase activity in the vacuolar system of ungerminated conidia, germinated conidia and appressoria, and we also describe the mechanism of lipid bodies uptake by ungerminated conidia vacuoles.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Lipid Mobilization and Acid Phosphatase Activity in Lytic Compartments during Conidium Dormancy and Appressorium Formation of Colletotrichum graminicola.

Schadeck

Leite

Buchi

1998

Cell Struct. Funct.

View full text Add to dashboard Cite

ABSTRACT. Colletotrichum graminicola, a pathogen of sorghum and corn, was investigated prior and during germination as to certain aspects of acid phosphatase activity and lipid mobilization. Ungerminated conidia cytoplasm was filled with lipid deposits, which were mobilized during the germination process. Cytochemical ultrastructural examination showedthat conidia vacuoles exhibit acid phosphatase activity, which is suggestive of lytic activity. Lipid bodies, stored in the ungerminated conidia cytoplasm, were internalized by vacuoles in a process analogous to microautophagy and were apparently digested inside them. The lipid bodies disappeared and vacuoles became enlarged in conidial cells during germination. Appressoria also showed acid phosphatase activity in multiple heterogeneous vesicles which were, in most cases, juxtaposed with lipid bodies. These results suggest that the vacuolar system plays an important role during C. graminicola germination and that the initial stages of lipid metabolization are taking place inside the vacuoles.

show abstract

Section: Discussionsupporting

confidence: 80%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Lipid Mobilization and Acid Phosphatase Activity in Lytic Compartments during Conidium Dormancy and Appressorium Formation of Colletotrichum graminicola.

Schadeck

Leite

Buchi

1998

Cell Struct. Funct.

View full text Add to dashboard Cite

show abstract

“…Colletotrichum graminicola (Ces.) Wilson (Politis & Wheeler, 1973) ; Erysiphe graminis DC. ex Merat (Bracker, 1968)], it seems unlikely that the uptake of materials from the host would be prevented, although haustoria may function for a short time only, translocation ceasing or being severely curtailed when the septum in the neck is mature.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of Septa in Dimargaris cristalligena R. K. Benjamin

Jeffries

Young

1979

Journal of General Microbiology

View full text Add to dashboard Cite

The fine structure of the septum in the vegetative hypha of the mycoparasite Dimargaris cristalligena is described. The cross-wall, continuous with the inner electron-lucent layer of the hyphal wall, bears a central pore with a flared margin occupied by a biumbonate electron-dense plug. A globose body, surrounded by a zone of ribosome-free cytoplasm, is situated next to the septal pore on each side of the cross-wall. In the neck of the haustorium, a globose body is normally observed only on that side of the septal pore next to the appressorium. Globose bodies appear structureless ; they are electron-dense when fixed in potassium permanganate and of variable electron density in aldehyde-osmium fixations. Cytoplasmic continuity is maintained through the septal pore by the plasmalemma. In the sporangiophore and sporiferous branchlets, the septal plug bears an upper globose protuberance and a lower obconic one. The hyphal septum in Tiegherniomyces californicus is ultrastructurally similar to that of D. cristalligena. The possible functional and taxonomic significance of such septa is indicated. I N T R O D U C T I O NDimargaris cristalligena, like other species in the genus, is parasitic on members of the Mucorales. Characteristically, it produces two-spored merosporangia and the hyphae are regularly septate. Liberated sporangiospores germinate on nutrient agar and the mycelium may make weak saprophytic growth in the absence of the host. Complex septa are produced in the mycelium, typically having a cross-wall with a flared central pore occluded by a plug, and usually with a spherical body on each side of the plug (Benjamin, 1959). Although the distinctive form of the septum found in the vegetative hyphae, as observed in the light microscope, is used as a diagnostic character of the Dimargaritaceae, the fine structure of such septa has not been described. Ultrastructural studies of septa (e.g. Moore & McAlear, 1962 ;Moore, 1965 ;MOSS, 1975 ;Khan, 1976) have provided substantial information supporting taxonomic relationships. The aim of this paper is to describe the fine structure of the septum of the vegetative hypha of D. cristalligena, with notes on the sporangiophore septum and on a related species Tieghenziomyces californicus, for comparison with other known forms of septa. METHODS Organisms

show abstract

“…Examples of pathogens employing this infection strategy are Colletotrichum graminicola Politis & Wheeler, 1973). Colletotrichum gloeosporioides (Ogle et al, 1990), and Colletotrichum lindemuthianum (Mercer et al, 1975;O'Connell et al, 1985).…”

Section: Colletotrichum Acutatum and Olive Fruits Interactionmentioning

confidence: 99%