Ultrastructure and Mineral Distribution in Heat-Damaged Rapeseed

Mills, J. T.; Chong, J.

doi:10.4141/cjps77-004

Cited by 14 publications

(14 citation statements)

References 7 publications

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“…The mature Candle seed coat (Fig. 1) consisted of epidermal, subepidermal, lignified palisade, crushed parenchyma, and aleurone layers, as previously described by Mills & Chong (1977). The epidermal layer contained mucilage which was extruded into the surrounding medium after the outer epidermal cell wall was ruptured (Van Caeseele et al 1981).…”

Section: Resultsmentioning

confidence: 84%

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Ultrastructural study of invasion of mature canola (Brassica campestris) seed byAspergittus amstelodami

Mills

Caeseele

Gillespie

1982

Canadian Journal of Plant Pathology

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Section: Resultsmentioning

confidence: 84%

“…The invasion of rapeseed or canola seeds by Aspergiilus species has not been studied at the electron microscope level. However ultrastructural studies have been carried out on rapeseed (Mills & Chong 1977;Stanley et al 1976;Von Hofsten 1974) and canola (Van Caeseele et al 1981). The fine structure of Aspergiilus spp.…”

mentioning

confidence: 99%

Ultrastructural study of invasion of mature canola (Brassica campestris) seed byAspergittus amstelodami

Mills

Caeseele

Gillespie

1982

Canadian Journal of Plant Pathology

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“…Also, phytate localization in the seeds of both species differs. Eighty percent of phytate phosphorus in rape seed (Mills and Chong, 1977) is distributed throughout the cotyledons and the rest is in the seed coat; its is also concentrated in substructures within protein bodies (globoids). In contrast to rape seed, soyabeans appear to have no definite site of phytate P localization (Tombs, 1967).…”

Section: Resultsmentioning

confidence: 99%

“…However, the rapeseed meal contains about 2 times as much total phosphorus than soyabean meal and a higher proportion of phytate P in total phosphorus than SBM (78 vs 64%; Rodehutscord et al, 1997). Also, the localization of phytate in seeds differs between both species (Tombs, 1967;Mills and Chong, 1977). These differences in distribution of phytate P may influence the susceptibility of phytates to hydrolysis by phytase.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic efficiency of plant and microbial phytase in cereal-rapeseed diets for growing pigs

Weremko¹,

Fandrejewski²,

Raj³

et al. 2001

J. Anim. Feed Sci.

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The experiment was carried out on 24 growing Polish Landrace pigs. The apparent digestibility of P from cereal grains (maize, barley, wheat, triticale, rye or mixture of barley with rye) and from diets containing cereals and 25% rapeseed meal (RSM) or 40% soyabean meal (SBM) was determined. The diets were unsupplemented or supplemented with microbial phytase (1000 FTU/kg). The digestibility of P was measured by an indirect method (with Cr 2 0 3 as an indicator) in 129 individual collections.The activity of intrinsic phytase in maize and oil meals was low (no more than 180 FTU/kg DM) and was considerably higher in other cereals (from 750 FTU in barley to 2077 FTU/kg DM in rye). The apparent digestibility of P in barley, wheat, triticale and rye did not differ between species (average 37.1%) and was about 17% percentage points higher (PO.01) than in maize.Although it was estimated that the effect of intrinsic and microbial phytase on digestibility of P was additive, plant phytase was 32 and 41% less effective than microbial phytase in diets with RSM and SBM, respectively. The intrinsic phytase content in cereal improved P digestibility in diets with RSM or SBM by 9.3-9.6% per 1000 FTU. Supplementation of diets with microbial phytase (1000 FTU) increased P digestibility by 13.7 and 16.3% in RSM and SBM diets, respectively. However, the increase in the content of digestible P by microbial phytase was similar (about 1 g/kg) in diets with both protein sources.

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“…In one particular study on aqueous extracts of rapeseed meals, glucosinolates were found in two of the four isolated protein fractions (Lo and Hill 1972). Relatively high level of sulfur were found within protein bodies of the rapeseed structure by various workers using energy-dispersive X-ray microanalysis (Hofsten 1974;Mills and Chong 1977 …”

Section: Pected the Above Findings Indicated Thatmentioning

confidence: 99%

CHROMATOGRAPHIC AND MICROSCOPIC DETECTION OF GLUCOSINOLATES IN RAPESEED USING N,2,6-TRICHLORO-p- BENZOQUINONEIMINE

Yiu¹,

Collins²,

Fulcher³

et al. 1984

Can. J. Plant Sci.

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A new histochemical method for detecting glucosinolates in rapeseed (Brassica napus L. ’Midas’) methanolic extracts and rapeseed (Brassica campestris L. ’Candle’) hand-cut sections has been developed using N,2,6-trichloro-p-benzoquinoneimine (TCQ) as the staining reagent. The specificity of TCQ was established by the following experiments. (1) TCQ was used as a spray reagent for detecting thio sugars separated from (a) crude and (b) partially purified rapeseed extracts on silica gel coated chromatograms. By comparison, the reaction of TCQ was relatively more selective than that of alkaline silver nitrate as it detected fewer spots on the chromatogram. (2) Rapeseed extracts and glucosinolate (GS) standards were analyzed on the same plate by one- and two-dimensional chromatography. After spraying with TCQ two of the three spots originated from the extract had the same color (yellow) reaction and similar Rf values as those of 3-butenyl GS and 2-hydroxy-3-butenyl GS. (3) Gas chromatography was used to identify the TCQ-reactive compounds present in the rapeseed extract. 3-butenyl GS and 2-hydroxy-3-butenyl GS were present as major constituents while 4-pentenyl GS, 2-hydroxy-4-pentenyl GS and an hydroxyindolylmethyl GS formed the minor entity. The use of TCQ for detecting glucosinolates in situ was established by the following microscopic studies. Hand-cut sections were prepared, stained with TCQ and examined microscopically. Protein bodies found inside cells of the cotyledon were the only stained (yellow in color) structures. The number of yellow-colored protein bodies decreased if the sections were left untreated at room temperature for more than 10 min prior to staining. Treatment of the sections with distilled water, 70% methanol or myrosinase before staining diminished the stainability of the protein bodies.Key words: Rapeseed structure, glucosinolates, chromatographic analyses, N,2,6-trichloro-p-benzoquinoneimine

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Ultrastructure and Mineral Distribution in Heat-Damaged Rapeseed

Abstract: MIr-rs, J. T. eNo CnoNc, J. 197'7 . Ultrastructure

Cited by 14 publications

References 7 publications

Ultrastructural study of invasion of mature canola (Brassica campestris) seed byAspergittus amstelodami

Ultrastructural study of invasion of mature canola (Brassica campestris) seed byAspergittus amstelodami

Enzymatic efficiency of plant and microbial phytase in cereal-rapeseed diets for growing pigs

CHROMATOGRAPHIC AND MICROSCOPIC DETECTION OF GLUCOSINOLATES IN RAPESEED USING N,2,6-TRICHLORO-p- BENZOQUINONEIMINE

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