Ultrastructure and molecular phylogenetics of a new isolate of Pleistophora pagri sp. nov. (Microsporidia, Pleistophoridae) from Pagrus pagrus in Egypt

Morsy, Kareem; Abdel-Ghaffar, Fathy; Mehlhorn, Heinz; Bashtar, Abdel-Rahman; Abdel‐Gaber, Rewaida

doi:10.1007/s00436-012-3012-9

Cited by 16 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, the infection rate was high (44%), which is comparable to the other studies with an infection rate of 32.1% for H. saurida from S. undosquamis in the Arabian Gulf (3), 29.23% and 6.15% for H. lessepsianus from S. lessepsianus in winter and summer months, respectively, in the Red Sea (Egypt) (26), and also an infection rate of 30.6% for Pleistophorapagri from Pagruspagrus in the Red Sea, Egypt (27). These reports showed that marine microsporidiosis follows a temperature and seasonal pattern and the infection rate of disease increases during the cold months of the year.…”

Section: Discussionsupporting

confidence: 85%

Microscopic and Molecular Description of the Microsporidian Parasite,Heterosporis sp., Infecting Lizardfish, Saurida undosquamis in the Persian Gulf

Larki

Peyghan

dezfuli

2021

Jentashapir J Cell Mol Biol

View full text Add to dashboard Cite

Background: The lizardfish is an economically important fish in the Persian Gulf with high rates of parasitic infections. Microsporidia species, as opportunistic parasites, cause several disorders, which in turn result in economic problems. Objectives: The main objective was to evaluate Heterosporis sp. infection in Persian Gulf lizardfish using the small subunit ribosomal RNA phylogenetics to describe and classification of the unknown microsporidia species as well as morphological characteristics. Methods: The abdominal cavities of fifty specimens of lizardfish, Saurida undosquamis, were examined using morphological and molecular techniques. Some irregular whitish cyst-like were fixed for histopathological and transmission electron observations. The small subunit ribosomal genomic DNA was studied and a 1,279 bp genomic sequence was amplified and investigated for molecular analysis. Results: Twenty-two (out of fifty) specimens were infected with irregular whitish microsporidian cysts. Light and electron microscopic findings revealed round cysts containing large numbers of monomorphic and ovoid spores with a posterior vacuole. Polar tube coiled between six and eight-times, in one row. The large xenoma (hypertrophied parasitizing host cells) was encapsulated by a host-derived thick connective tissue in pathological samples. The phylogenetic analysis showed that despite some morphological similarity of the Persian Gulf microsporidia sp. to Glugea spp., the most closely related species with minimum genetic distance to Heterosporis anguillarum isolated is Japanese eels (Anguillajaponica). Conclusions: This is the first phylogenetic report of microsporidian infections in mesenteric tissues of lizardfish S. undosquamis in Iran including morphological and molecular markers, to introduce novel species

show abstract

Section: Discussionsupporting

confidence: 85%

Microscopic and Molecular Description of the Microsporidian Parasite,Heterosporis sp., Infecting Lizardfish, Saurida undosquamis in the Persian Gulf

Larki

Peyghan

dezfuli

2021

Jentashapir J Cell Mol Biol

View full text Add to dashboard Cite

show abstract

“…In this study, the authors strongly advocate trypanosome taxonomy with molecular methods like SSU rDNA sequencing. Molecular analysis of the diversity of myxozoan parasites is currently based on investigations of the SSU rDNA (Krebes et al, 2010;Morsy et al, 2012;Whipps and Kent, 2006 and references therein). The SSU rDNA marker could even be used to detect cryptic myxozoan species within the same trout host (Percopsis omiscomaycus) but in different tissue types (Easy et al, 2005).…”

Section: Small (Ssu Rdna) and Large (Lsu Rdna) Subunit Ribosomal Genementioning

confidence: 99%

A Review of Molecular Approaches for Investigating Patterns of Coevolution in Marine Host–Parasite Relationships

Froeschke¹,

Heyden²

2014

Advances in Parasitology

View full text Add to dashboard Cite

“…Microsporidia are known to infect fresh, marine, and brackish water fish in their natural environment, as well as in experimental models which impart in decorative, games, and food purposes resulting significant economic losses due to liquified muscles, growth retardation, unhealthy flesh which renders it unfit for human consumptions (Lom et al,1995;Lom and Nilsen, 2003;Joh et al, 2007;Sanders et al, 2012). Microsporidian infection has been detected in different organs of fish like muscles (Fehlert et al, 2005;McGourty et al, 2007;Garhy et al, 2017), gills (Kummari, 2018), kidney (Didier et al, 2004), liver (Matos et al, 2003), ovary (Abidi et al, 2016), and peritoneal cavity (Casal et al, 2008;Ghaffar, 2012;Morsy et al, 2013), etc.…”

Section: Introductionmentioning

confidence: 99%

“…also reported the xenomic microsporidian infection in the gills of wrasse from western Norway andNylund et al (2011) reported the extensive necrosis due to Paranucleospora theridion in the gills of Atlantic salmon Salmo salar. The gills are the most susceptible organ for microsporidian infection as this organ is in direct contact with the water which favors horizontal transmission of infection asSanders et al (2012) andMorsy et al (2012) observed the horizontal transmission of microsporidian infection in rearing tanks of zebra fish and in Pagrus pagrus fish.Microsporidian infection was very minute in the muscle of the Channa punctatus, whileZhang et al (2010) reported microsporidian infection in the form of cyst in the trunk muscle of Pacific bluefin tuna.…”

mentioning

confidence: 99%

Morphological Identification of Microsporidian Parasite in Channa punctatus from River Gomti, Lucknow, Uttar Pradesh, India

Kamal,

Suman,

Swati

2023

Intern. J. Zool. Invest.

View full text Add to dashboard Cite

Channa punctatus from Gomti river, Lucknow, India were randomly collected and investigated for microsporidian parasites, 89 (64.96%) fishes were found infected. The infection was present in both symptomatic as well as in asymptomatic fishes. Symptomatic fish showed discoloration of body surface externally and internally whitish vesicles have been observed in kidney and liver with liquefied gills and gonads. Microsporidia infection has been detected in different organs of Channa punctatus by light microscopic techniques. Observation of fresh wet mounted smears showed microsporidian spores which appeared oval in shape illuminating green florescent with slight Brownian motion with different developmental stages. Giemsa stained smears also showed merogonic and sporogonic stages of the parasite with polar filament extrusion under pressure. Ultrastructural analysis through Scanning electron microscope further confirms the microsporidian infection based on spores' size and morphological appearance. Spores were oval to pyriform-shaped cell body having spongy external appearance and were found of two size ranges measuring 15.62 µm and 12.83 µm which might be non-xenomic Pliestophora species.

show abstract

Ultrastructure and molecular phylogenetics of a new isolate of Pleistophora pagri sp. nov. (Microsporidia, Pleistophoridae) from Pagrus pagrus in Egypt

Cited by 16 publications

References 65 publications

Microscopic and Molecular Description of the Microsporidian Parasite,Heterosporis sp., Infecting Lizardfish, Saurida undosquamis in the Persian Gulf

Microscopic and Molecular Description of the Microsporidian Parasite,Heterosporis sp., Infecting Lizardfish, Saurida undosquamis in the Persian Gulf

A Review of Molecular Approaches for Investigating Patterns of Coevolution in Marine Host–Parasite Relationships

Morphological Identification of Microsporidian Parasite in Channa punctatus from River Gomti, Lucknow, Uttar Pradesh, India

Contact Info

Product

Resources

About