Ultrastructure change and transcriptome analysis of GA3 treatment on seed germination of moso bamboo(<i>Phyllostachys edulis</i>)

Li, Juan; Bai, Yucong; Xie, Yali; Gao, Jian

doi:10.1080/15592324.2022.2091305

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…In our study, the starch content of the seeds treated with or without GA 3 significantly decreased with the germination process, and the starch content of the seeds treated with GA 3 was significantly lower than that of the seeds treated without GA 3 (Figure 6C) in the radicle emergence stage. Similar results were obtained by Li et al (2022), who found that GA 3 treatment accelerated the hydrolysis of starch in Phyllostachys edulis seeds. Consistent with the present results, the expression levels of two key starch-hydrolases, beta-amylase (SgBAM) and alpha-glucosidase (SgAGLU), were significantly up-regulated in the Bg group (Figure 6A).…”

Section: Role Of Carbohydrate-related Metabolism Pathways During Ga 3...supporting

confidence: 88%

“…GAs also enhance the growth potential of the embryo by antagonizing ABA activity ( Finch-Savage and Leubner-Metzger, 2006 ). The molecular mechanisms of GA 3 -induced seed dormancy release have been explored using transcriptomic and/or metabolomic analysis in Fraxinus hupehensis ( Song et al., 2019 ), Leymus chinensis ( Li et al., 2021 ), Phyllostachys edulis ( Li et al., 2022 ), Panax notoginseng ( Ge et al., 2023 ), and Zea mays ( Han et al., 2023 ). However, the mechanism of seed dormancy release in different plants might be species-dependent ( Chibani et al., 2006 ; Lee et al., 2006 ; Pawłowski, 2007 ; Pawłowski, 2009 ; Deng et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis reveals the potential mechanism of GA3-induced dormancy release in Suaeda glauca black seeds

Wang,

Xu,

et al. 2024

Front. Plant Sci.

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Suaeda glauca Bunge produces dimorphic seeds on the same plant, with brown seeds displaying non-dormant characteristics and black seeds exhibiting intermediate physiological dormancy traits. Previous studies have shown that black seeds have a very low germination rate under natural conditions, but exogenous GA3 effectively enhanced the germination rate of black seeds. However, the physiological and molecular mechanisms underlying the effects of GA3 on S. glauca black seeds are still unclear. In this study, transcriptomic profiles of seeds at different germination stages with and without GA3 treatment were analyzed and compared, and the TTF, H2O2, O2–, starch, and soluble sugar contents of the corresponding seed samples were determined. The results indicated that exogenous GA3 treatment significantly increased seed vigor, H2O2, and O2– contents but decreased starch and soluble sugar contents of S. glauca black seeds during seed dormancy release. RNA-seq results showed that a total of 1136 DEGs were identified in three comparison groups and were involved mainly in plant hormone signal transduction, diterpenoid biosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, and carbohydrate metabolism pathway. Among them, the DEGs related to diterpenoid biosynthesis (SgGA3ox1, SgKAO and SgGA2ox8) and ABA signal transduction (SgPP2Cs) could play important roles during seed dormancy release. Most genes involved in phenylpropanoid biosynthesis were activated under GA3 treatment conditions, especially many SgPER genes encoding peroxidase. In addition, exogenous GA3 treatment also significantly enhanced the expression of genes involved in flavonoid synthesis, which might be beneficial to seed dormancy release. In accordance with the decline in starch and soluble sugar contents, 15 genes involved in carbohydrate metabolism were significantly up-regulated during GA3-induced dormancy release, such as SgBAM, SgHXK2, and SgAGLU, etc. In a word, exogenous GA3 effectively increased the germination rate and seed vigor of S. glauca black seeds by mediating the metabolic process or signal transduction of plant hormones, phenylpropanoid and flavonoid biosynthesis, and carbohydrate metabolism processes. Our results provide novel insights into the transcriptional regulation mechanism of exogenous GA3 on the dormancy release of S. glauca black seeds. The candidate genes identified in this study may be further studied and used to enrich our knowledge of seed dormancy and germination.

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Section: Role Of Carbohydrate-related Metabolism Pathways During Ga 3...supporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals the potential mechanism of GA3-induced dormancy release in Suaeda glauca black seeds

Wang,

Xu,

et al. 2024

Front. Plant Sci.

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“…Key metrics for assessing seed germination and vigor include germination rate, potential, index, and vigor index. 20 , 21 This study found that both acid and Al stress significantly impeded S. davidii seed germination. Under strong acid conditions (pH = 3.5), there was a marked decline in germination rate, index, and vigor compared to the control, indicating a suppression of germination ability and seedling vigor in a highly acidic environment.…”

Section: Discussionmentioning

confidence: 66%

Effects of acid and aluminum stress on seed germination and physiological characteristics of seedling growth in Sophora davidii

Long,

Xie,

Zhao

et al. 2024

Plant Signaling & Behavior

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Sophora davidii , a vital forage species, predominantly thrives in the subtropical karst mountains of Southwest China. Its resilience to poor soil conditions and arid environments renders it an ideal pioneer species for ecological restoration in these regions. This study investigates the influence of acidic, aluminum-rich local soil on the germination and seedling growth physiology of S. davidii . Experiments were conducted under varying degrees of acidity and aluminum stress, employing three pH levels (3.5 to 5.5) and four aluminum concentrations (0.5 to 2.0 mmol·L −1 ). The results showed that germination rate, germination index, and vigor index of S. davidii seeds were decreased but not significantly under slightly acidic conditions (pH 4.5–5.5), while strong acid (pH = 3.5) significantly inhibited the germination rate, germination index, and vigor index of white spurge seeds compared with the control group. Aluminum stress (≥0.5 mmol·L −1 ) significantly inhibited the germination rate, germination index, and vigor index of S. davidii seed. Moreover, the seedlings’ root systems were sensitive to the changes of aluminum concentration, evident from significant root growth inhibition, characterized by root shortening and color deepening. Notably, under aluminum stress (pH = 4.3), the levels of malondialdehyde and proline in S. davidii escalated with increasing aluminum concentration, while antioxidant enzyme activities demonstrated an initial increase followed by a decline. The study underscores the pivotal role of cellular osmoregulatory substances and protective enzymes in combating aluminum toxicity in S. davidii , a key factor exacerbating growth inhibition in acidic environments. These findings offer preliminary theoretical insights for the practical agricultural utilization of S. davidii in challenging soil conditions.

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“…Especially, the α-Xyl and β-D-Xyl weakened the endosperm cell wall by modulating the structure of xyloglucan during seed germination [28,29]. And the decomposition of starch could promote seed sprouting [30]. Thus, these proteins, which were related to metabolism, might paly crucial role in seed germination.…”

Section: Discussionmentioning

confidence: 99%

Integrated multi-omics approach to reveal the molecular networks underlying the seed germination of Polygonatum cyrtonema

Xu,

Wang,

Chen

et al. 2023

Preprint

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Background Polygonatum cyrtonema (P. cyrtonema), a famous traditional medicinal resource for over two thousand years in China, is in high demand with the vigorous development of the health industry. The rhizome propagation could easily lead to germplasm degradation, severe pests, diseases, etc., and thus, the seed propagation method is a superior alternative to artificially cultivating seedlings on a large scale. However, the seeds of P. cyrtonema need at least two years dormancy period under natural circumstances to undergo morphological and physiological maturation, and germination of seeds has not been fully understood. To reveal the mechanisms of hormone crosstalk and metabolic process regulating germination, transcriptomic, proteomic and plant hormonomics analyses of P. cyrtonema seeds in three different germinating stages (the fresh mature seed, named S1, the emergence of radicle, named S2 and the phase between primary rhizome and cotyledon emergence, named S3) were performed. Results Transcriptome analysis revealed that 11,565 differentially expressed genes (DEGs) between S1 and S2, 10,196 between S1 and S3, 7,696 between S2 and S3. Proteome analysis revealed 647 differentially expressed proteins (DEPs) between S1 and S2, 1720 between S1 and S3, and 1475 between S1 and S3. Comprehensive transcriptomic and proteomic analysis indicated that metabolism and plant hormone signal transduction might be the main pathways involved in seed germination of P. cyrtonema. In addition, several proteins, including abscisic acid receptor PYL4 (PYL4) and protein phosphatase 2C (PP2C) bind ABA to form PYL-ABA-PP2C complex, associated with ABA signaling, were down-regulated between S1 and S3. And the sucrose synthase (SUS), involving in the antagonism between ABA and GA, decreased between S1 and S3. While, these proteins such as beta-fructofuranosidase (INV), alpha-xylosidase (α-Xyl), beta-D-xylosidase (β-D-Xyl) and beta-glucosidase (bglX), related to starch and sucrose metabolism, were up-regulated between S1 and S3. Hormone content detection showed that ABA, GAs, IAA, JA and SA decreased significantly, while, the ratio of GAs/ABA increased distinctly from S1 to S3, suggesting that transduction in plant hormones, especially ABA-GA antagonism might regulate seed germination. Conclusion The current study showed a possible schematic and a novel insight to explain the dynamic seed germination mechanism of P. cyrtonema, and emphasized that phytohormone cross-talk, ABA signaling and shydrolysis of starch to glucose might play an important role in regulating seed germination.

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Ultrastructure change and transcriptome analysis of GA3 treatment on seed germination of moso bamboo(Phyllostachys edulis)

Cited by 5 publications

References 38 publications

Comparative transcriptome analysis reveals the potential mechanism of GA3-induced dormancy release in Suaeda glauca black seeds

Comparative transcriptome analysis reveals the potential mechanism of GA3-induced dormancy release in Suaeda glauca black seeds

Effects of acid and aluminum stress on seed germination and physiological characteristics of seedling growth in Sophora davidii

Integrated multi-omics approach to reveal the molecular networks underlying the seed germination of Polygonatum cyrtonema

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