Ultrastructure changes induced by dry film formation of a trisodium phosphate blend, antimicrobial solution

Lea, Peter J.; Ding, Shao; Lemez, S. B.

doi:10.1002/sca.4950250602

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…To reduce the damage inherent in these treatments, various innovative sample preparation methods have been developed (Lea et al 2003;Anderson et al 2004;Salvenmoser et al 2010;Dohnalkova et al 2011). In this study, we report the systematic, intuitive observation of bacterial ultrastructure by e-PL using FESEM and TEM.…”

Section: Introductionmentioning

confidence: 93%

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Cell envelope disruption of E. coli Exposed to ϵ‐polylysine by FESEM and TEM technology

Zhou

Xiang-yang

2013

Scanning

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To investigate the action mechanism of ϵ-polylysine (ϵ-PL) against Escherichia coli (E. coli), a new field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) technology has been developed. The log phase E. coli cells were first incubated with ϵ-PL for 8 min, then the samples were directly added onto the silicon platelet and the copper grid, followed by a simple in situ fixation and freezing dehydration. FESEM and TEM were used to examine the ultrastructure changes in the bacterial envelope which was affected by ϵ-PL. various damages of E. coli cell envelope by ϵ-PL have demonstrated the detachment of outer membrane, the swelling of inner membrane, the apical burst of cells and the leakage of cytosol at a minimal inhibitory concentration (MIC) concentration. It also exhibited whole cell lysis at double MIC concentration. In summary, the new FESEM and TEM technology and appropriate sample preparation protocols have been found to be useful for investigating the biocidal activity of ϵ-PL against E. coli.

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Section: Introductionmentioning

confidence: 93%

“…To reduce the damage inherent in these treatments, various innovative sample preparation methods have been developed (Lea et al . ; Anderson et al . ; Salvenmoser et al .…”

Section: Introductionmentioning

confidence: 99%

Cell envelope disruption of E. coli Exposed to ϵ‐polylysine by FESEM and TEM technology

Zhou

Xiang-yang

2013

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“…Trisodium phosphate's (TSP) high alkalinity in solution enables removal of fat films to allow more contact and also destruction of fatty molecules in the bacterial cell membrane, thus causing leakage of its contents (Oyarzabal 2005). It is approved by the USDA for use in food processing, including meat preservation (Lea and others 2003). Studies have shown that spray‐washing with TSP could reduce contamination of beef briskets and prevent bacterial attachment, hence allowing easier removal of bacteria during washing (Gorman and others 1997).…”

Section: Meat Preservation Technologiesmentioning

confidence: 99%

Intervention Technologies for Ensuring Microbiological Safety of Meat: Current and Future Trends

Chen¹,

Ren²,

Seow³

et al. 2012

Comp Rev Food Sci Food Safe

104

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This article reviews current and future techniques that are applied in the meat industry to ensure product safety. Consumer demand for high-quality food and raised economic standards have triggered the development of emergent technologies to replace traditional well-established preservation processes. Some promising nonthermal and thermal technologies, such as chemical and biological interventions, high hydrostatic pressure (HHP), irradiation, active packaging, natural antimicrobials and microwave, radiofrequency, and steam pasteurization, are under consideration for the preservation of meat products. All these alternative technologies are designed to be mild, energy-conserving, environmentally friendly, and maintaining natural appearance and flavor, while eliminating pathogens and spoilage microorganisms. Their combination, as in the hurdle theory, may improve their effectiveness for decontamination. The objective of this article is to reflect on the possibilities and especially the limitations of the previously mentioned technologies.

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“…Resolution of a severe infection may require prescription drugs, salves, or foot soakings. Arrowroot, baking soda, basil oil, tea tree oil, sage oil, and clove oil are also reported to inhibit both aerobic bacteria and yeast-mold-fungi growths respectively [ 1 - 7 , 9 - 11 , 13 , 15 - 19 , 21 - 23 ].…”

Section: Introductionmentioning

confidence: 99%

A novel aromatic oil compound inhibits microbial overgrowth on feet: a case study

Misner¹

2007

Journal of the International Society of Sports Nutrition

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Background: Athlete's Foot (Tinea pedis) is a form of ringworm associated with highly contagious yeast-fungi colonies, although they look like bacteria. Foot bacteria overgrowth produces a harmless pungent odor, however, uncontrolled proliferation of yeast-fungi produces small vesicles, fissures, scaling, and maceration with eroded areas between the toes and the plantar surface of the foot, resulting in intense itching, blisters, and cracking. Painful microbial foot infection may prevent athletic participation. Keeping the feet clean and dry with the toenails trimmed reduces the incidence of skin disease of the feet. Wearing sandals in locker and shower rooms prevents intimate contact with the infecting organisms and alleviates most foot-sensitive infections. Enclosing feet in socks and shoes generates a moisture-rich environment that stimulates overgrowth of pungent both aerobic bacteria and infectious yeast-fungi. Suppression of microbial growth may be accomplished by exposing the feet to air to enhance evaporation to reduce moistures' growthstimulating effect and is often neglected. There is an association between yeast-fungi overgrowths and disabling foot infections. Potent agents virtually exterminate some microbial growth, but the inevitable presence of infection under the nails predicts future infection. Topical antibiotics present a potent approach with the ideal agent being one that removes moisture producing antibacterialantifungal activity. Severe infection may require costly prescription drugs, salves, and repeated treatment.

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Ultrastructure changes induced by dry film formation of a trisodium phosphate blend, antimicrobial solution

Cited by 3 publications

References 9 publications

Cell envelope disruption of E. coli Exposed to ϵ‐polylysine by FESEM and TEM technology

Cell envelope disruption of E. coli Exposed to ϵ‐polylysine by FESEM and TEM technology

Intervention Technologies for Ensuring Microbiological Safety of Meat: Current and Future Trends

A novel aromatic oil compound inhibits microbial overgrowth on feet: a case study

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