Ultrastructure, in vitro and in vivo, of staphylococci exposed to antibiotics

Lorian, Víctor; Atkinson, Barbara; Waluschka, Alexandra; Kim, Yehum

doi:10.1007/bf01566867

Cited by 13 publications

(10 citation statements)

References 7 publications

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“…Thus, the cell walls of bacteria recovered directly from the sputum of cystic fibrosis patients (3) or from infected urine (105) or burn fluids (4) exhibit iron depletion and differ radically from those of cells of the same organism grown in batch culture in vitro. Similar bacterial surface changes are seen in response to alterations in growth rate (67,118), exposure to subinhibitory concentrations of certain antibiotics (66), and growth on solid surfaces (67). Equally profound differences in enzyme activity have been noted between bacterial cells adherent to surfaces anti planktonic cells of the same organism (C. S. Dow, R. Whittenbury & D. Kelly, unpublished).…”

Section: Physiology Of Biofilm Bacteriamentioning

confidence: 79%

Bacterial Biofilms in Nature and Disease

Costerton¹,

Cheng²,

Geesey³

et al. 1987

Annu. Rev. Microbiol.

2,824

1,177

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Section: Physiology Of Biofilm Bacteriamentioning

confidence: 79%

Bacterial Biofilms in Nature and Disease

Costerton¹,

Cheng²,

Geesey³

et al. 1987

Annu. Rev. Microbiol.

2,824

1,177

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“…These enzymes regulate cell walls, cell division, septation, elongation, and cell shape (14) and thus alter the surface morphology and structure of the bacteria. Each ␤-lactam antibiotic has its own binding affinity for these enzymes, called penicillin-binding proteins, and since penicillinbinding proteins vary among different bacterial species, a variety of morphological alterations and types of damage can occur (7)(8)(9). Evidence concerning the actions of ␤-lactam antibiotics has been obtained not only biochemically but also by means of the direct observation of morphological alterations by optical microscopy and scanning electron microscopy (SEM), and investigators have performed a series of studies on this subject (3,5,10,11).…”

mentioning

confidence: 99%

Atomic Force Microscopy: Application to Investigation of Escherichia coli Morphology before and after Exposure to Cefodizime

Braga

Ricci

1998

Antimicrob Agents Chemother

132

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Atomic force microscopy (AFM) is a recently developed technique that allows for the investigation of the surface morphology of a biological specimen at an unprecedented level of resolution. The aim of the present study was to explore some of the new opportunities offered by AFM by studying the morphological and surface alterations induced inEscherichia coli by supra-MICs and sub-MICs of a beta-lactam antibiotic (cefodizime). The underlying principle of AFM is the scanning and sensing of the topography of a sample by means of near-field microscopy that makes it possible to obtain simultaneous digital measurements of the x, y, andz coordinates of any point on the bacterial surface with great resolution (x andy, ∼20 Å; z, ∼1 Å). Unlike scanning electron microscopy, performance of AFM does not require a vacuum, drying to the critical point, or the coating of the bacterial surface with a metal layer. The digital storage of the information makes it easy to rotate the image, observe the bacterial surface and induced structural alterations from different points of view, and obtain a cross-section at any desired point with precise, automatic measurement of the heights and sizes of normal versus damaged bacteria. Use of the new and outstanding technique of AFM will make it possible for researchers to investigate biological samples immersed in biological fluids and will also make it possible for them to study the morphological alterations of living bacteria exposed to antibiotics as they are taking place.

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“…The staphylococci grown in broth containing 0.09 ,ug of cloxacillin per ml were 1.2 to 1.6 ,um in diameter. Most contained one thick cross wall, and a few contained two cross walls; these cells were comparable to staphylococci grown in broth containing oxacillin or penicillin (10,13).…”

Section: Resultsmentioning

confidence: 77%

“…Such cells have also been observed in vivo in the peritoneal fluid of infected mice treated with oxacillin (10) and in sputa of patients treated with beta-lactam antibiotics (11). The purpose of the current investigation Was to examine the morphology and distribution of staphylococci in experimental endocarditis of rabbits treated with a low dose of cloxacillin.…”

mentioning

confidence: 93%