Ultrastructure of 26 Cases of Ki-1 Lymphomas: Morphoimmunologic Correlation

Abstract

Twenty-six cases of high-grade lymphomas with activation markers (CD30) classified and immunophenotyped according to the Kiel classification were studied to determine their fine structural features. Transmission electron microscopy showed in 17 cases anaplastic nuclear and cytoplasmic changes identical to those observed in Hodgkin's disease, it being impossible to determine by the morphology a B, T, or null nature. Four high-grade B-centroblastic and immunoblastic cases and five T-pleomorphic cases showed nuclear changes and cytoplasmic differentiation that suggested a T or B nature. An immunogold-labeling technique showed CD30-positive particles primarily in the Golgi complex and occasionally in the cell membrane.

CD30+ TIA-1+ ALK+ Anaplastic Large Cell Lymphoma: Studies of Three Cases by Flow Cytometry Analysis, Immunohistochemistry and Electron Microscopy

“…Only a few reports on ultrastructural findings [9,23,24] or results with colloidal immunogold labeling technique in ALCL have ever been described. Rivas et al [23] emphasized that the ultrastructure of ALCL cases showed nuclear and cytoplasmic changes suggestive of cellular activation with evolved nuclear shapes.…”

“…Rivas et al [23] emphasized that the ultrastructure of ALCL cases showed nuclear and cytoplasmic changes suggestive of cellular activation with evolved nuclear shapes. Our observations of three cases confirmed these modifications, and simultaneously showed that the ultrastructural differences were not remarkable among the three different morphologic variants of ALKpositive ALCL.…”

“…Clinically, ALCL can be subdivided into primary systemic (ALK positive and ALK negative), primary cutaneous and secondary subtypes [6,7,27,29]. Ultrastructurally, the cellular activation and cytotoxic granules have been demonstrated in ALCL, although there were different issues regarding the cytotoxic granules [9,23,24]. Felgar et al [9] reported three types of cytotoxic granules, type I (dense core), type II (multivesicular), and intermediate type, whereas Sadahira et al [24] found only type II cytotoxic granules in the cases of ALCL.…”

CD30 Expression bei Patienten mit einer Mycosis fungoides unter Berücksichtigung der inter- und intraindividuellen Variabilität sowie histologischen und klinischen Parametern

“…Das dominierende Färbemuster der CD30 Expression war in unserem Kollektiv die Zytoplasmafärbung, sowohl epidermal als auch dermal (89%). (Rivas et al 1990). Es wurden einige Fallberichte veröffentlicht, in denen alle CD30-positiven Zellen eine Golgi-und Membranfärbung (Papadimitriou et al 1996), eine starke Golgi-und Membranfärbung (Murphy et al 2005;Ma et al 2010;Lobo et al 2017) oder überwiegende Golgifärbung (Kikukawa et al 2003) aufwiesen.…”