Ultrastructure of a Spindle Cell Carcinoma in the Mammary Gland of a Dog

Kusewitt, Donna F.; Hahn, F. F.; Muggenburg, B.A.

doi:10.1177/030098589202900212

Cited by 5 publications

(1 citation statement)

References 3 publications

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“…The studied cell lines belonged to different subcategories of mammary tumors: CMT-U27 representing a simple carcinoma and CMT-U309 representing a spindle cell carcinoma ( 20 , 21 ). Simple carcinomas are composed of only one cell type, resembling either luminal epithelial or myoepithelial cells ( 22 ), while spindle cell carcinomas of the breast typically contain clusters of elongated cells, which resemble more mesenchymal than epithelial type of cells and are rare and aggressive subtypes of mammary tumors both in human and dog ( 23 – 25 ). As expected, analysis of these two cell lines with several lineage-specific markers revealed clear differences between the cell lines, showing that spindle carcinoma and simple carcinoma cells expressed predominantly basal or luminal cytokeratin markers, respectively.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Contractility and Invasion Potential of Two Canine Mammary Tumor Cell Lines

2017

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Cancer cells are surrounded by a mechanically and biochemically distinct microenvironment that undergoes dynamic changes throughout the neoplastic progression. During this progression, some cancer cells acquire abnormal characteristics that potentiate their escape from the primary tumor site, to establish secondary tumors in distant organs. Recent studies with several human cancer cell lines have shown that the altered physical properties of tumor cells, such as their ability to apply high traction forces to the surroundings, are directly linked with their potential to invade and metastasize. To test the hypothetical interconnection between actomyosin-mediated traction forces and invasion potential within 3D-microenvironment, we utilized two canine mammary tumor cell lines with different contractile properties. These cell lines, canine mammary tumor (CMT)-U27 and CMT-U309, were found to have distinct expression patterns of lineage-specific markers and organization of actin-based structures. In particular, CMT-U309 carcinoma cells were typified by thick contractile actomyosin bundles that exerted high forces to their environment, as measured by traction force microscopy. These high contractile forces also correlated with the prominent invasiveness of the CMT-U309 cell line. Furthermore, we found high contractility and 3D-invasion potential to be dependent on the activity of 5′AMP-activated protein kinase (AMPK), as blocking AMPK signaling was found to reverse both of these features. Taken together, our findings implicate that actomyosin forces correlate with the invasion potential of the studied cell lines.

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Section: Introductionmentioning

confidence: 99%

Analysis of Contractility and Invasion Potential of Two Canine Mammary Tumor Cell Lines

2017

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Historical Control Data of Spontaneous Lesions in Beagle Dogs.

et al. 1994

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Abstract:A variety of spontaneous lesions found histologically in control Beagle dogs (males: 91, famales; 97) used in toxicity studies that were conducted at the Biosafety Research Center, Foods, Drugs and Pesticides during a 10 year period, are described.All animals were exsanguinated by incision of the axillary artery under sodium pentobarbital anesthesia at the end of each study. Neoplastic lesions included fibrosarcoma of the heart in a female dog at approximately 27 weeks of age (1.0%) and hemangioendothelioma of the spleen in a male dog, also at approximately 27 weeks of age (1.1%).The most common non-neoplastic changes in both male and famale dogs were deposits of amor phous masses at the renal papilla (males; 68.1%, females; 80.4%) and fetal glomerulus in the kidney (males; 53.8%, females; 50.5%). Fatty changes in the renal tubular epithelium of female dogs occurred at an incidence of greater than 50%. Pigment deposition in the spleen, guranulation of the liver, atrophy in the thymus, C-cell hyperplasia of the thyroid gland, and pituitary cysts in male and female dogs were evident at an incidence of greater than 10%. In addition, multinucleated giant cells in the testes, eosinophilic bodies in the renal tubular epithelium in males, cellular infiltration of the parotid gland, and deposits of calcium in the spinal cord of females were seen at incidences of greater than 10%. Atrophy of the thymus and C-cell hyperplasia of the thyroid increased with age. Fetal glomerulus of kidney decreased with age.Other rare, but interesting lesions included: ectopic stomach tissue in the ileum, ductro-insular proliferation of the pancreas, deposits of calcium at the beginning portion of the aorta and in the spinal cord, and hemangiectasis at the atrioventriclar valve of the heart. (J Toxicol Pathol 7: 329-343, 1994)

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Fibrosarcoma of the heart in a beagle dog.

Kobayashi

Yamakawa

1994

J Toxicol Pathol

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Ultrastructure of a Spindle Cell Carcinoma in the Mammary Gland of a Dog

Cited by 5 publications

References 3 publications

Analysis of Contractility and Invasion Potential of Two Canine Mammary Tumor Cell Lines

Analysis of Contractility and Invasion Potential of Two Canine Mammary Tumor Cell Lines

Historical Control Data of Spontaneous Lesions in Beagle Dogs.

Fibrosarcoma of the heart in a beagle dog.

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