Ultrastructure of Bone and Cartilage Formed in vivo in Diffusion Chambers

I, Bab; Cr, Howlett; Ba, Ashton; Owen, Maureen

doi:10.1097/00003086-198407000-00038

Cited by 59 publications

(23 citation statements)

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“…Our system thus directly models the precise spatial determinants of cell differentiation operating in development. Interestingly, whereas cartilage only forms occasionally in open transplants of BMSCs Kuznetsov et al, 1997;Martin et al, 1997), cartilage and bone tissues that do form in closed transplantation systems (diffusion chambers) (Ashton et al, 1980;Bab et al, 1984;Gundle et al, 1995) are organized in a spatial pattern similar to the one we observe in vitro (cartilage in the interior, bone in the periphery). It is plausible that a gradient of oxygen tension (Ashton et al, 1980;Scott, 1992) might be directly involved in the generation of the spatial pattern observed in both systems.…”

Section: Discussionsupporting

confidence: 60%

Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells

Muraglia

Corsi

Riminucci

et al. 2003

Journal of Cell Science

130

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Bone-marrow stromal cells can differentiate into multiple mesenchymal lineages including cartilage and bone. When these cells are seeded in high-density 'pellet culture', they undergo chondrogenesis and form a tissue that is morphologically and biochemically defined as cartilage. Here, we show that dual chondro-osteogenic differentiation can be obtained in the same micromass culture of human bone-marrow stromal cells. Human bone-marrow stromal cells were pellet cultured for 4 weeks in chondro-inductive medium. Cartilage 'beads' resulting from the micromass culture were then subcultured for further 1-3 weeks in osteo-inductive medium. This resulted in the formation of a distinct mineralized bony collar around hyaline cartilage. During the chondrogenesis phase, type I collagen and bone sialoprotein were produced in the outer portion of the cartilage bead, which, upon subsequent exposure to beta-glycerophosphate, mineralized and accumulated extracellular bone sialoprotein and osteocalcin. Our modification of the pellet culture system results in the formation of a chondro-osseous 'organoid' structurally reminiscent of pre-invasion endochondral rudiments, in which a bony collar forms around hyaline cartilage. The transition from a cell culture to an organ culture dimension featured by our system provides a suitable model for the dissection of molecular determinants of endochondral bone formation, which unfolds in a precisely defined spatial and temporal frame

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Section: Discussionsupporting

confidence: 60%

Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells

Muraglia

Corsi

Riminucci

et al. 2003

Journal of Cell Science

130

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“…This beginning of osteogenesis leads to the peripheral bridging of the primary chondroid tissue islets. Strikingly, it is contemporaneous with the development of numerous blood vessels within the mesenchyme, "suggesting that the differentiation of the stem cells into osteoblasts is initiated or influenced by the increased oxygen tension in their surroundings (Hall 1969;Thorogood 1979;Bab et al 1984). Indeed, as we have already stated regarding the cranial vault, no vessels are visible in the immediate vicinity of the calcified primordium at the earliest embryonic stages (Lengel6 et al 1990).…”

Section: Discussionmentioning

confidence: 85%

Chondroid tissue in the early facial morphogenesis of the chick embryo

1996

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The calcified tissues involved in the early morphogenesis of the so-called intramembranous bones of the facial skeleton were studied by microradiographic and histological techniques in 22 chick embryos at the 9th, 12th and 14th days of incubation. On the 9th day, the bones of the upper face and palatal vault are made up of thin sheets of chondroid tissue, deposited in their respective mesenchymal condensations. Woven and lamellar bone formation subsequently takes place in each of them from the 12th day of incubation, mainly on the external side of their chondroid primordia. The same phenomena occur in the lower facial and mandibular bones. These facts indicate that the primitive facial desmocranium of the chick embryo, which is classically considered to be formed by intramembranous ossification, first consists of chondroid tissue. As in the cranial vault, this tissue thus represents the initial modality of the skeletogenic differentiation within the avian facial mesenchyme.

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“…When transplanted in an isolated environment in diffusion chambers in vivo, they can form calcified tissue. This tissue, although avascular, resembles the skeletal osseous tissue by both light and electron microscopic evaluation (Ashton et al 1980, Bab et al 1984. Not all of these colonies grown in vitro can give rise to bone as Friedenstein (1980) has shown; but a significant proportion, about one third, do.…”

Section: Osteogenic Progenitor Cellsmentioning

confidence: 93%

Initiation and enhancement of bone formation: A review

Triffitt

1987

Acta Orthopaedica Scandinavica

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Ultrastructure of Bone and Cartilage Formed in vivo in Diffusion Chambers

Cited by 59 publications

References 0 publications

Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells

Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells

Chondroid tissue in the early facial morphogenesis of the chick embryo

Initiation and enhancement of bone formation: A review

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