Ultrastructure of coronary microvessels during heart reperfusion after prolonged ischemia under conditions of various forms of artificial hypothermia

Abstract

It has been found that, in children with Roger's disease corrected under conditions of two funda mentally different procedures of anesthetic management, myocardial reperfusion after cardiac arrest under artificial hypothermic circulation is accompanied with obstruction of more than 70% of coronary bed microvessels, one third of them being blocked with hydropic endothelial cells or their cystiform fragments. It hampers or even totally prevents their functioning in the postoperative period. The content of necrotic cells increases, while the three "working" cell types demonstrate a decrease in myocropinocytotic transport. Cir culatory arrest during perfusionless hypothermia and immersion reperfusion do not result in a dramatic change of general morphology of microvessels as compared to the control group maintaining the endothelial cell population unaltered. Ultrastructural organization of endothelial microvessels displayed no features of intracellular regeneration. However, a heterogenic response of the structures responsible for transendothelial transfer of macromolecules provides the basis for the recovery of endothelium structure and function, as a patient's temperature reaches the standard value.

High-resolution visualization of mouse cardiac microvasculature using optical histology

“…A standard method to directly visualize the cardiac microvasculature, either in vivo [15] or ex vivo, currently does not exist. To date, methods of visualizing the cardiac microvasculature primarily used electron microscopy [16,17] and histology [18], both of which enable visualization of the fine ultrastructure of ex vivo microvessels, but do not enable a wide-field view of the cardiac microvasculature.…”