Ultrastructure of Early Secondary Embryogenesis by Multicellular and Unicellular Pathways in Cork Oak (Quercus suber L.)

Puigderrajols, Pere; Mir, Gisela; Molinas, Marisa

doi:10.1006/anbo.2000.1317

Cited by 55 publications

(45 citation statements)

References 33 publications

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“…The proliferative mass is a translucent, fully undifferentiated, nonvascularized tissue that develops in the hypocotyls of the recurrent somatic embryos. Cork tree somatic embryogenesis has been carefully characterized at anatomical and ultrastructural levels in previous works (Puigderrajols et al, 1996(Puigderrajols et al, , 2001. Neither suberin deposition nor multilamellated cell walls could be detected in somatic embryos using optical and fluorescent microscopy techniques or by electron microscopy.…”

Section: Cork Subtractive Librarymentioning

confidence: 99%

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

Soler

Serra²,

Molinas³

et al. 2007

Plant Physiology

143

139

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Cork (phellem) is a multilayered dead tissue protecting plant mature stems and roots and plant healing tissues from water loss and injuries. Cork cells are made impervious by the deposition of suberin onto cell walls. Although suberin deposition and cork formation are essential for survival of land plants, molecular studies have rarely been conducted on this tissue. Here, we address this question by combining suppression subtractive hybridization together with cDNA microarrays, using as a model the external bark of the cork tree (Quercus suber), from which bottle cork is obtained. A suppression subtractive hybridization library from cork tree bark was prepared containing 236 independent sequences; 69% showed significant homology to database sequences and they corresponded to 135 unique genes. Out of these genes, 43.5% were classified as the main pathways needed for cork biosynthesis. Furthermore, 19% could be related to regulatory functions. To identify genes more specifically required for suberin biosynthesis, cork expressed sequence tags were printed on a microarray and subsequently used to compare cork (phellem) to a non-suberin-producing tissue such as wood (xylem). Based on the results, a list of candidate genes relevant for cork was obtained. This list includes genes for the synthesis, transport, and polymerization of suberin monomers such as components of the fatty acid elongase complexes, ATP-binding cassette transporters, and acyltransferases, among others. Moreover, a number of regulatory genes induced in cork have been identified, including MYB, No-Apical-Meristem, and WRKY transcription factors with putative functions in meristem identity and cork differentiation.

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Section: Cork Subtractive Librarymentioning

confidence: 99%

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

Soler

Serra²,

Molinas³

et al. 2007

Plant Physiology

143

139

View full text Add to dashboard Cite

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“…The embryogenic ones are characterised by their small size, with rich cytoplasm and filled with starch. The similar character of embryogenic cells was described in Passiflora cincinnata (Rocha et al, 2011) and cork oak (Puigderrajols et al, 2001). Cells with the same characteristics were described for the embryogenic parts of the explants of Carya (Rodriguez & Wetzstein, 1998).…”

Section: Meristematic and Embryogenic Cells Within Explantsmentioning

confidence: 55%

“…al., 2007). Cork oak somatic embryos are of a multicellular origin or a single-cell origin depending on the explant cells which participated in the embryo's formation (Puigderrajols et al, 2001). The single-and multicellular origins of somatic embryos was also described (among others) in Borago officinalis (Quinn et al, 1989), Camellia japonica (Barciela & Vieitez, 1993), Elaeis guinnesis (Schwendiman et al, 1990) and www.intechopen.com…”

Section: Introductionmentioning

confidence: 99%

Cellular Markers for Somatic Embryogenesis

Kurczyńska¹,

Potocka²,

Dobrowolska³

et al. 2012

Embryogenesis

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“…But embryo origin is difficult to determine exactly (Faure et al, 1996), in spite of the numerous anatomical slices analyzed. In any case, a dual origin has been already been suggested for somatic embryos (Barciela & Vieitz, 1993;Puigderrojols et al, 2001;Williams & Maheswaran, 1986). Embryogenic cells were minute, resembled meristematic cells, displayed more densely staining nuclei and nucleoli, denser cytoplasm and starch grains, and a thick cell wall.…”

Section: Discussionmentioning

confidence: 75%

In vitro regeneration of Didymopanax morototoni

2006

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The present study aimed at establishing a complete plant regeneration protocol for Didymopanax morototoni (matchwood), a native Brazilian forest species. Four types of explants (root, shoot, node, and cotyledonary leaves) were obtained from in vitro germinated seeds. In the first step, woody plant medium (WPM) with casein hydrolysate (250 mgL ). Twenty days after inoculation, the material was evaluated. Embryogenic calli were split, transferred to expression medium with several combinations of NAA and KIN, and moved to fresh medium after 60 days. Light did not interfere in embryo expression. Somatic embryos were formed either from individual cells or cell clusters. Plantlets were obtained in WPM medium and 10 gL -1 of sucrose with no plant regulator, or using 0.1 mgL -1 BAP and 0.5 mgL -1 GA. Plantlets from somatic embryos of D. morototoni developed in 33% of the cases.Keywords: somatic embryo, woody plants, embryogenesis, plantlets, Schefflera (Didymopanax), Araliaceae. RESUMO Regeneração in vitro de Didymopanax morototoniO presente estudo visou o estabelecimento de um completo protocolo de regeneração para Didymopanax morototoni (morototó, caixeta) uma espécie florestal nativa do Brasil. Quatro tipos de explantes (raiz, caule, nódulo foliar e folha cotiledonar) foram obtidos a partir de sementes germinadas. Na primeira etapa, meio WPM com caseína hidrolisada (250 mgL -1 ) e 2,4D (1,0 e 5,0 mgL -1 ) foram usados em combinação com cinetina (0,1 ou 1,0 mg L -1 ). Vinte dias depois de inoculado, o material foi avaliado. Calos embriogênicos foram divididos e transferidos para meio de expressão com várias combinações de ácido naftaleno-acético e cinetina, e repicados a cada 60 dias para meio novo. A luz não interferiu na expressão embriogênica. Embriões somáticos foram formados ou de células individuais ou de agregados de células. As plântulas foram obtidas no meio WPM com 10 g L -1 de sacarose e sem reguladores de crescimento ou com 0,1 mg L -1 de Benzil-adenina e 0,5 mg L -1 de giberelina. O desenvolvimento das plântulas a partir de embriões somáticos de D. morototoni foi alcançada em 33% dos casos.Palavras-chave: embrião somático, plantas lenhosas, embriogênese, Schefflera (Didymopanax), Araliaceae.

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Ultrastructure of Early Secondary Embryogenesis by Multicellular and Unicellular Pathways in Cork Oak (Quercus suber L.)

Cited by 55 publications

References 33 publications

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

A Genomic Approach to Suberin Biosynthesis and Cork Differentiation

Cellular Markers for Somatic Embryogenesis

In vitro regeneration of Didymopanax morototoni

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