Ultrastructure of ferritin in the leaves of Mesembryanthemum crystallinum under stress conditions

Paramonova, N. V.; Shevyakova, N. I.; Кузнецов, Вл. В.

doi:10.1134/s1021443707020136

Cited by 10 publications

(2 citation statements)

References 30 publications

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“…At T2, amyloplasts are dramatically shrunken and appear as dislocated bodies that still contain numerous crystal structures but no more starch (Figures 4E, 4K, 4F and 4L). The crystal structures accumulating in degenerating amyloplast strongly resemble to the crystalloid ferritin structures previously observed in chloroplast of Mesembryanthemum crystallinum subjected to salt stress (Paramonova et al, 2007). They accumulate under both LN and HN conditions with aging (Figures 4E, 4K, 4F and 4L).…”

Section: Resultssupporting

confidence: 69%

Multi-scale phenotyping of senescence-related changes in roots ofRapeseedin response to nitrogen deficiency

James,

Masclaux-Daubresse,

Balliau

et al. 2024

Preprint

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Senescence related markers have been widely studied in leaves in many plant species. Root senescence is more difficult to characterize. The existence of two different root organs in B. napus, with a taproot that appear to be specifically dedicated to the storage and remobilization of nutrients, offered the possibility of analysing the temporality of the changes linked to aging, based on the degradation of the taproot reserves. Microscopic and biochemical analyses showed that taproot plays an important role in carbon and nitrogen storage as reflected by the large quantities of starch and proteins present at early development stages. The proteomic study associated to the description of biochemical, morphological and anatomic changes provides a comprehensive picture of the main events occurring in the taproot and in the lateral roots with aging. Master modifications as protein and cell wall degradation, amino acid catabolism versus synthesis, nucleic acid degradation are presented and senescence related markers specific or not of the root types were identified. Comparison with Arabidopsis public data facilitated the identification of markers common to root and leaf senescence. The analysis of protease changes provides a list of candidates that may play a role in nitrogen and carbohydrate remobilization from taproot to the shoot and flowering organs and that would deserve attention for further functional analyses.

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Section: Resultssupporting

confidence: 69%

Multi-scale phenotyping of senescence-related changes in roots ofRapeseedin response to nitrogen deficiency

James,

Masclaux-Daubresse,

Balliau

et al. 2024

Preprint

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“…Fe-EDDHA supply to the plants provoked an increased abundance of Fe in most of the mesophyll cells as well as the appearance of small sized Fe rich granules in plastids. These Fe deposits were attributed to Fe-ferritin structures, based on the size, 1 μm, (Paramonova et al, 2007), the exact match with ferritin proteins detected by immunofluorescence (Figure 4) and the fact that these structures were absent in a fer1,3,4 triple mutant devoid of ferritin proteins in leaves (Figure 3). Interestingly, ferritin-iron was more abundant in the cells associated to the vascular system.…”

Section: Discussionmentioning

confidence: 98%

New insights into Fe localization in plant tissues

Roschzttardtz¹,

Conéjéro²,

Divol³

et al. 2013

Front. Plant Sci.

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Deciphering cellular iron (Fe) homeostasis requires having access to both quantitative and qualitative information on the subcellular pools of Fe in tissues and their dynamics within the cells. We have taken advantage of the Perls/DAB Fe staining procedure to perform a systematic analysis of Fe distribution in roots, leaves and reproductive organs of the model plant Arabidopsis thaliana, using wild-type and mutant genotypes affected in iron transport and storage. Roots of soil-grown plants accumulate iron in the apoplast of the central cylinder, a pattern that is strongly intensified when the citrate effluxer FRD3 is not functional, thus stressing the importance of citrate in the apoplastic movement of Fe. In leaves, Fe level is low and only detected in and around vascular tissues. In contrast, Fe staining in leaves of iron-treated plants extends in the surrounding mesophyll cells where Fe deposits, likely corresponding to Fe-ferritin complexes, accumulate in the chloroplasts. The loss of ferritins in the fer1,3,4 triple mutant provoked a massive accumulation of Fe in the apoplastic space, suggesting that in the absence of iron buffering in the chloroplast, cells activate iron efflux and/or repress iron influx to limit the amount of iron in the cell. In flowers, Perls/DAB staining has revealed a major sink for Fe in the anthers. In particular, developing pollen grains accumulate detectable amounts of Fe in small-size intracellular bodies that aggregate around the vegetative nucleus at the binuclear stage and that were identified as amyloplasts. In conclusion, using the Perls/DAB procedure combined to selected mutant genotypes, this study has established a reliable atlas of Fe distribution in the main Arabidopsis organs, proving and refining long-assumed intracellular locations and uncovering new ones. This “iron map” of Arabidopsis will serve as a basis for future studies of possible actors of iron movement in plant tissues and cell compartments.

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Polyamines and Plant Adaptation to Saline Environments

Кузнецов

Shevyakova

2009

Desert Plants

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Ultrastructure of ferritin in the leaves of Mesembryanthemum crystallinum under stress conditions

Cited by 10 publications

References 30 publications

Multi-scale phenotyping of senescence-related changes in roots ofRapeseedin response to nitrogen deficiency

Multi-scale phenotyping of senescence-related changes in roots ofRapeseedin response to nitrogen deficiency

New insights into Fe localization in plant tissues

Polyamines and Plant Adaptation to Saline Environments

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