Ultrastructure of<i>Synchytrium endobioticum</i>resting spores and enhancement of germination using snails

Hampson, Michael C.; Yang, A. F.; Bal, Arya K.

doi:10.1080/00275514.1994.12026477

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…For instance, species of Allomyces (Machlis & Ossia 1953, Skucas 1968, Olson 1984, Youatt 1991a and Blastocladiella (Cantino 1969) are reported to survive as resistant sporangia, and Allomyces is well known to survive air-drying (Youatt 1991b). Thickwalled sporangia may function as survival structures in Olpidium (Campbell 1985, Weber & Webster 2000, and Synchytrium is known to form resting spores (Laidlaw 1985, Hampson et al 1994. The survival structures are unknown for Rhizophlyctis (Dogma 1974, Willoughby 2001 and rumen chytrids (Davies et al 1993a, b, Theodorou et al 1994 though resting spores of R. rosea were illustrated by Dogma (1974).…”

Section: Introductionmentioning

confidence: 99%

Some Chytridiomycota in soil recover from drying and high temperatures

Gleason

Letcher

McGee

2004

Mycological Research

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Section: Introductionmentioning

confidence: 99%

Some Chytridiomycota in soil recover from drying and high temperatures

Gleason

Letcher

McGee

2004

Mycological Research

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“…Upon infection, the fungus induces its host to produce tumour-like outgrowth (warts) on tubers, stolons and sprouts. Warts consist of hypertrophic host cells each containing a single sporangium, which host the infectious zoospores (Hampson 1985;Hampson et al 1994). In senescing wart tissue infectious zoospores are no longer produced and thick-walled resting sporangia or winter spores are formed and able to survive inter-host periods for up to 20 years (Hampson 1993).…”

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Improved real-time PCR assay for detection of the quarantine potato pathogen, Synchytrium endobioticum, in zonal centrifuge extracts from soil and in plants

Gent-Pelzer¹,

Krijger²,

Bonants³

2009

Eur J Plant Pathol

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Development of PCR-based Detection Methods for the Quarantine Phytopathogen Synchytrium endobioticum, Causal Agent of Potato Wart Disease

Boogert

Gent-Pelzer²,

Bonants³

et al. 2005

Eur J Plant Pathol

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PCR-based methods were developed for the detection and quantification of the potato pathogen Synchytrium endobioticum in soil extracts and in planta. PCR primers, based on the internal transcribed spacer region of the multi-copy gene rDNA were tested for specificity, sensitivity and reproducibility in conventional and real-time PCR assays. Soil extraction procedures compared included the Hendrickx centrifugation (HC) procedure, nested wet sieving (NWS) and a method used by the Plant Protection Service (PPS). The primers amplified a 472 bp product from S. endobioticum DNA, but did not amplify DNA from other potato pathogens, other plant pathogens, and related species. Standard cell disruption and DNA extraction and purification methods were optimized for amplification of S. endobioticum DNA from resting sporangia. DNA was successfully amplified from a single sporangium and equivalent DNA preparations from soil extracts. Low levels of target DNA in water did not amplify, possibly due to DNA loss during final purification steps. A real-time PCR assay, developed for soil-based extracts using primers and probe based on the rDNA gene sequences, involved co-amplification of target DNA along with an internal DNA fragment. Both conventional and real-time PCR methods performed well with HC-and NWS-extracts having a threshold sensitivity of 10 sporangia per PCR assay. Of the three soil extraction methods, only with the HC method could 100 g soil samples be efficiently processed in one single PCR assay. Such a high capacity assay could be useful for routine soil analysis in respect to disease risk assessments and to secure de-scheduling according to EPPO guidelines.

show abstract

Ultrastructure ofSynchytrium endobioticumresting spores and enhancement of germination using snails

Cited by 4 publications

References 18 publications

Some Chytridiomycota in soil recover from drying and high temperatures

Some Chytridiomycota in soil recover from drying and high temperatures

Improved real-time PCR assay for detection of the quarantine potato pathogen, Synchytrium endobioticum, in zonal centrifuge extracts from soil and in plants

Development of PCR-based Detection Methods for the Quarantine Phytopathogen Synchytrium endobioticum, Causal Agent of Potato Wart Disease

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