Ultrastructure of Kupffer cells and hepatocytes in the Dubin-Johnson syndrome: A case report

Sobaniec-Łotowska, M; Lebensztejn, Dariusz Marek

doi:10.3748/wjg.v12.i6.987

Cited by 15 publications

(7 citation statements)

References 13 publications

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“…Extensive brown pigmentation was present in perivenular hepatocytes. 2 Immunolabelling of the tissue was positive for MRP3 (a basolateral transport protein), indicating the possibility of Rotor's syndrome (RS) or Dubin -Johnson syndrome (DJS). Because of insufficient tissue, MRP2 immunolabelling could not be performed.…”

Section: Resultsmentioning

confidence: 99%

Novel mutations in the Dubin–Johnson syndrome gene ABCC2/MRP2 and associated biochemical changes

Devgun¹,

El-Nujumi²,

O'Dowd

et al. 2012

Ann Clin Biochem

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A patient with sepsis and jaundice was admitted for diagnosis and treatment. Associated biochemical changes included increased C-reactive protein, conjugated bilirubin and gamma-glutamyltransferase, the duration of which was protracted. High urine coproporphyrin isomer-1 and immunostaining of liver tissue suggested Dubin-Johnson syndrome. DNA sequencing using polymerase chain reaction amplification of the ABCC2 gene revealed the patient to have a compound heterozygous variant of MRP2, a molecule involved in canalicular transport of bilirubin. There was a history of jaundice since infancy.

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Section: Resultsmentioning

confidence: 99%

Novel mutations in the Dubin–Johnson syndrome gene ABCC2/MRP2 and associated biochemical changes

Devgun¹,

El-Nujumi²,

O'Dowd

et al. 2012

Ann Clin Biochem

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“…Such areas in the oocyte cytoplasm may represent endoplasmic reticulum swelling [29] and [31]. Swollen endoplasmic reticulum and mitochondria were already described in oocytes [28] and [32] luteal cells [6] and [9] and other cell types [8] and [11].…”

Section: Discussionmentioning

confidence: 98%

Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

et al. 2009

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The present study aimed to test different cryoprotectants on cryopreservation of pig ovarian tissue. Pig ovaries (n = 3) were collected at a local slaughterhouse. From each ovary, ten cortex samples were taken. One was immediately fixed (control) and another placed in short-term tissue incubation (STTI control). The other 8 samples were cryopreserved, in pairs, using 4 different cryoprotectants: dimethyl sulphoxide (Me2SO -1.5 M), ethylene glycol (EG -1.5 M), propanediol (PROH -1.5 M) and glycerol (GLY -10%), all with 0.4% sucrose. Samples were slow cooled and stored in liquid nitrogen for 7 days. After thawing and cryoprotectant removal, one sample from each treatment was immediately fixed and the other was placed in short-term tissue incubation (STTI) for 2 h and then fixed. Samples were processed for histology and transmission electron microscopy. The percentages of morphologically normal follicles (MNF) in cryopreserved tissue using Me2SO (67.0 ± 4.9), EG (81.8 ± 1.4) and PROH (55.9 ± 9.9) were significantly lower (P < 0.05) than observed in fresh control tissue (97.7 ± 1.2). When ovarian tissue was cryopreserved with GLY, no morphologically normal follicles could be found (0%). After STTI, PROH showed a significantly lower percentage of MNF when compared with all other treatments and the control. After ultrastructural analysis, follicles cryopreserved with Me2SO and EG showed some small alterations, but no signs of advanced degeneration. Overall, these were similar to follicles from the control group. In conclusion, it is possible to cryopreserve preantral follicles from pig ovarian tissue using Me2SO or EG.

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“…Kupffer cells are resident macrophages in liver and function as scavengers for cellular debris and blood-born material entering liver. The potential source of the pigment in Kupffer cells seen histologically could be retention of bile, lipofuscin, erythrophagocytosis and disintegrated hepatocytes (Sobaniec-Lotowska and Lebensztejn 2006 ). Therefore, histological changes, including Kupffer cell pigmentation and hepatocyte apoptosis, could correspond to transcriptional pathways related to cell damage response.…”

Section: Discussionmentioning

confidence: 99%

Toxicogenomic assessment of liver responses following subchronic exposure to furan in Fischer F344 rats

et al. 2015

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Furan is a widely used industrial chemical and a contaminant in heated foods. Chronic furan exposure causes cholangiocarcinoma and hepatocellular tumors in rats at doses of 2 mg/kg bw/day or greater, with gender differences in frequency and severity. The hepatic transcriptional alterations induced by low doses of furan (doses below those previously tested for induction of liver tumors) and the potential mechanisms underlying gender differences are largely unexplored. We used DNA microarrays to examine the global hepatic mRNA and microRNA transcriptional profiles of male and female rats exposed to 0, 0.03, 0.12, 0.5 or 2 mg/kg bw/day furan over 90 days. Marked gender differences in gene expression responses to furan were observed, with many more altered genes in exposed males than females, confirming the increased sensitivity of males even at the low doses. Pathway analysis supported that key events in furan-induced liver tumors in males include gene expression changes related to oxidative stress, apoptosis and inflammatory response, while pathway changes in females were consistent with primarily adaptive responses. Pathway benchmark doses (BMDs) were estimated and compared to relevant apical endpoints. Transcriptional pathway BMDs could only be examined in males. These median BMDs ranged from 0.08 to 1.43 mg/kg bw/day and approximated those derived from traditional histopathology. MiR-34a (a P53 target) was the only microRNA significantly increased at the 2 mg/kg bw/day, providing evidence to support the importance of apoptosis and cell proliferation in furan hepatotoxicity. Overall, this study demonstrates the use of transcriptional profiling to discern mode of action and mechanisms involved in gender differences.Electronic supplementary materialThe online version of this article (doi:10.1007/s00204-015-1561-2) contains supplementary material, which is available to authorized users.

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Ultrastructure of Kupffer cells and hepatocytes in the Dubin-Johnson syndrome: A case report

Cited by 15 publications

References 13 publications

Novel mutations in the Dubin–Johnson syndrome gene ABCC2/MRP2 and associated biochemical changes

Novel mutations in the Dubin–Johnson syndrome gene ABCC2/MRP2 and associated biochemical changes

Cryopreservation of swine ovarian tissue: Effect of different cryoprotectants on the structural preservation of preantral follicle oocytes

Toxicogenomic assessment of liver responses following subchronic exposure to furan in Fischer F344 rats

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