Ultrastructure of metacercarial cysts of six heterophyid trematodes from fish

Armitage, Mark H.

doi:10.1007/pl00008518

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…Some Heterophyidae and Bucephalidae species immediately harbor and encyst in the gill while others search the proximities of calliperest gill blood vessels where they produce a multilayered CPC (Armitage 2000). This aspect may be related to the low irrigation and nutritive diffusion of this kind of connective tissue and its relative isolation of the immune system (Thomson 1983;Gartner and Hiatt 1999).…”

Section: Discussionmentioning

confidence: 99%

Gill histopathology of Maria-da-toca Hypleurochilus fissicornis by metacercariae of Bucephalus margaritae (Digenea: Bucephalidae)

et al. 2014

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Gills of Maria-da-toca Hypleurochilus fissicornis collected at Ponta do Sambaqui-Florianópolis island-Brazil, were analyzed to describe the histopathology caused by metacercaria of Bucephalus margaritae. Gills were submitted to the routine histological techniques for embedding in paraffin and permanent mounting in Balsam and stereoscopic analysis. Metacercariae showed a branchial infection site pattern for encystations. The branchial infection site pattern is half-basalward in the primary branchial filament with amplitude of the infection of 1-3 metacercaria. Cysts occurred within branchial abductor muscle and cartilaginous and osseous tissues of the gills. Each metacercariae had a contentional hyaline parasitic capsule and melanin-like pigmentation. The half-apicalward region of the primary branchial filaments showed several dysplasia degrees, cartilage and osseous degeneration (pyknosis), thrombosis and immune exudated cells (mainly lymphocytes). Cytopathologies as thickening of the epithelium lining of the secondary branchial filaments were a response of the branchial infection site pattern of the metacercaria. Interlamellar obliteration and fusion of the lamellae due to the hypertrophy and hyperplasia of the epithelial lining as well as chloride cells occurred. Pyknosis of pillar cells and epithelial lining cells from the secondary branchial filaments were also present. Bucephalosis in H. fissicornis gills is no-hemorrhagic and no-fatal branchitis, but could compromises the gill functions and could permits the secondary opportunistic infections.

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Section: Discussionmentioning

confidence: 99%

Gill histopathology of Maria-da-toca Hypleurochilus fissicornis by metacercariae of Bucephalus margaritae (Digenea: Bucephalidae)

et al. 2014

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Parasites of Fishes

Kent

Fournie²,

Baker

2007

Flynn's Parasites of Laboratory Animals

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Artifacts from Rapid Microwave Processing of Trematode Tissues (Ascocotyle pachycystisandleighi)

Armitage¹

2004

Microscopy Today

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The use of microwave energy to assist in the processing of biological tissues for microscopy has generated significant interest in recent years. Microwave (MW) processing has been used to prepare tissues for light microscopy (Carranza et al. 1990 [using parasite tissues]; van Dorp et al. 1995; Davis et al. 1997; Izumi et al 2000; and Rohr et al. 2001), as well as for electron microscopy (Kasa et al. 1982; Hopwood et al. 1984; Leong et al. 1985; Kang et al, 1991 [using parasite tissues]; Heumann 1992; Wagenaar et al. 1993; Login and Dvorak 1993; Giberson and Demaree 1995; Madden and Miriam 1997; Giberson et al. 1997; Morin et al. 1997; Petrali and Mills 1999; Massa and Arana-Chavez 2000; Hernandez and Guillen 2000 [using parasite tissues]; Demaree 2001; Giberson 2001).Most reports, particularly those published by manufacturers of microwave ovens, have shown positive results regarding tissue ultrastructure, however discussion continues on possible mechanisms of preservation by the use of MW technology.

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Ultrastructure of metacercarial cysts of six heterophyid trematodes from fish

Cited by 6 publications

References 12 publications

Gill histopathology of Maria-da-toca Hypleurochilus fissicornis by metacercariae of Bucephalus margaritae (Digenea: Bucephalidae)

Gill histopathology of Maria-da-toca Hypleurochilus fissicornis by metacercariae of Bucephalus margaritae (Digenea: Bucephalidae)

Parasites of Fishes

Artifacts from Rapid Microwave Processing of Trematode Tissues (Ascocotyle pachycystisandleighi)

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