Ultrastructure of odontoblasts in kitten tooth germs as revealed by freeze-fracture

Sasaki, Takahísa; Nakagawa, Katsuhiro; Higashi, Shohei

doi:10.1016/0003-9969(82)90048-6

Cited by 30 publications

(24 citation statements)

References 21 publications

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“…The presence of albumin and a,HS-glycoprotein in multivesicular bodies, the endocytosis of DNP-RSA by 2 hours after its intravenous injection, and the negligible amounts of albumin detected in enamel, taken together, indicate that the baso-lateral surface of ameloblasts represents an active site of protein uptake. The presence of the tracer in the multivesicular bodies of various mineralized tissue cells (i.e., ameloblasts, odontoblasts, osteoblasts) and soft tissue cells further suggests that endocytosis of (Sasaki et al, 1982) and material can, therefore, conceptually pass around them. Coll, collagen fibril; m, mitochondria.…”

Section: Uptake Of Plasma Proteins By Ameloblastsmentioning

confidence: 96%

Endocytotic functions of ameloblasts and odontoblasts: Immunocytochemical and tracer studies on the uptake of plasma proteins

Nanci¹,

Fortin²,

Ghitescu³

1996

Anat. Rec.

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Section: Uptake Of Plasma Proteins By Ameloblastsmentioning

confidence: 96%

Endocytotic functions of ameloblasts and odontoblasts: Immunocytochemical and tracer studies on the uptake of plasma proteins

Nanci¹,

Fortin²,

Ghitescu³

1996

Anat. Rec.

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“…Mature odontoblasts exhibit distal junctional structures constituted by adherens, gap, and tight junctions. Although adherens and gap junctions appear early between odontoblasts (Sasaki et al, 1982), there is no information on the appearance and development of tight junctions between odontoblasts during early dentinogenesis. Studies using electron opaque tracers have shown that tracers do not penetrate between odontoblasts in early stages of dentinogenesis, thus suggesting the presence of tight junctional structures (AranaChavez and .…”

mentioning

confidence: 99%

“…Studies using electron opaque tracers have shown that tracers do not penetrate between odontoblasts in early stages of dentinogenesis, thus suggesting the presence of tight junctional structures (AranaChavez and . The majority of previous studies have shown that tight junctions are of the focal or macular type (Sasaki et al, 1982;Iguchi et al, 1984;Calle, 1985;Köling, 1988), but others have claimed that these junctions form a belt around the cells (Bishop, 1985;Bishop and Yoshida, 1992 osteoblasts during the initial phases of intramembranous osteogenesis (Soares et al, 1992;.…”

mentioning

confidence: 99%

Development of tight junctions between odontoblasts in early dentinogenesis as revealed by freeze-fracture

Arana‐Chavez

Katchburian

1997

Anat. Rec.

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Background Mature odontoblasts possess junctional structures constituted by adherens, gap, and tight junctions. Although adherens and gap junctions appear early between odontoblasts, there is no information on the appearance and development of tight junctions between odontoblasts. In this study, we have examined freeze‐fracture replicas of early dentinogenesis to study the development of tight junctions between odontoblasts and to determine whether these junctions are of zonular or macular type. Methods Upper first molar tooth germs of Wistar rats between 1 and 3 days old were fixed in buffered 4% glutaraldehyde/4% formaldehyde and subsequently cryoprotected with cacodylate‐buffered glycerol. Freeze‐fracture replicas were obtained in a Balzers 301 apparatus, and early stages of dentinogenesis were examined in a Jeol 100 CX II electron microscope. Results In the stage of early dentine matrix prior to mineralization, odontoblasts exhibit only gap junctions. With the progression of development, the distal plasma membranes of odontoblasts show numerous short tight junctions formed by fused particles and grooves. In the stage of advanced mineralization, branched and continuous rows of fused particles or grooves constitute tight junctions of the focal or macular type. Conclusions The present study shows that tight junctions of focal or macular type appear on distal plasma membrane of early odontoblasts during differentiation. Formation of tight junctions indicates the establishment of a distal membrane domain and maturation of odontoblasts. These events occur as mantle dentine formation ceases and circumpulpar dentine formation begins. Anat. Rec. 248:332‐338, 1997. © 1997 Wiley‐Liss, Inc.

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“…These zonular tight junctions have never been described previously between odontoblasts. In freezefracture, Sasaki et al [1982] only reported macular tight junctions on kitten odontoblasts and Koling et al [ 19811 did not observe any tight junctions in human odontob lasts. Our results do not agree with the previous studies of Koling et al [1981], possibily because of technical difficulties involved in using this method on mineralized tissue.…”

Section: Discussionmentioning

confidence: 99%

Intercellular Junctions Between Human Odontoblasts

Callé¹

1985

Cells Tissues Organs

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Intercellular junctions in the odontoblastic layer have been studied with a freeze-fracture technique. Children’s tooth germs were fixed, sliced and deminerahzed. Samples of the pulpodentinal border were routinely prepared for freeze-fracture. Three kinds of intercellular junctions were detected between human odontoblast cell bodies: gap junctions, desmosomes and tight junctions. Numerous gap junctions are responsible for intercellular communication at different levels of the cell bodies. Focal tight junctions, parallel to the axis of the cell, and desmosomes are sites of cell-to-cell adhesion between lateral plasma membranes. At the distal end of the cell bodies, junctional complexes consist of zonular tight junctions and gap junctions. These zonular tight junctions, never before described between odontoblasts, contribute to the pseudo-epithelial organization of the odontoblastic layer. They constitute a predentin-pulp barrier, the permeability of which must be studied to establish their role in relation to dentin formation.

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Ultrastructure of odontoblasts in kitten tooth germs as revealed by freeze-fracture

Cited by 30 publications

References 21 publications

Endocytotic functions of ameloblasts and odontoblasts: Immunocytochemical and tracer studies on the uptake of plasma proteins

Endocytotic functions of ameloblasts and odontoblasts: Immunocytochemical and tracer studies on the uptake of plasma proteins

Development of tight junctions between odontoblasts in early dentinogenesis as revealed by freeze-fracture

Intercellular Junctions Between Human Odontoblasts

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