Ultrastructure of the cell walls of two closely related clostridia that possess different regular arrays of surface subunits

Abstract

Stock cultures were maintained on BBL TSE medium (16), consisting of 1% tryptone (Oxoid), 0.1% sucrose, 0.02% FeSO4 7H2O, 0.04% (0.02%) Na2SO3, 0.008% Na2S203 5H20, and 0.7% agar (Oxoid). The higher concentration (0.04%) of Na2SO3 was used for the cultivation of strain L111-69, and the lower concentration (0.02%) was used for strain D120-70, which grew better at low sulfite concentration. Special precautions to eliminate atmospheric oxygen 869 on July 10, 2020 by guest

Ultrastructure and Partial Characterization of a Regular Array in the Cell Wall of Lactobacillus brevis

“…Among gram-positive bacteria, regular arrays have been found in a few genera such as Bacillus (11)(12)(13)19), Clostridium (12,23) and Micrococcus (31). In addition, we have already shown a tetragonal array in the outermost cell wall layer of Lactobacillus fermentum (15).…”

Chemical characterization of the regularly arranged surface layer glycoprotein of Clostridium thermosaccharolyticum D120-70

“…SDSjPAGE was carried out according to Laemmli [24] on 10% slab gels [25]. Freeze-etching and thin-sectioning electron microscopy was performed as previously described [4].…”

“…Oneand two-dimensional nuclear magnetic resonance studies, together with methylation analysis and periodate oxidation, were used to determine the structures of the polysaccharide portions of these glycopeptides. The combined chemical and spectroscopic evidence suggests the following structures : In the course of the structural characterization of the surface layers of the taxonomically closely related thermophilic eubacteria Clostridium thermosaccharolyticum D120-70 and Clostridium thermohydrosulfuricum Ll l l-69 [4], chemical analysis provided first evidence for the presence of glycoprotein subunits in the surface layer arrays of these strains [5]. Since then the structures of the carbohydrate portions of surface-layer glycoproteins of C. thermohydrosulfuricum Llll-69 [6] and Bacillus stearothermophilus NRS 2004/3a [7, 81 have been elucidated and the nature of the linkage region of one of these glycopeptides has been described [9].…”

A novel type of cell wall structure with two periodic layers (S-layer) of a Bacillus sp. strain KL1

“…The MW obviously represents the PG-layer and because the S-layers consist of protein or glycoprotein paracrystals both the OW and IW could be of this origin. This type of cell wall structure has been described before for some Clostridium species [8][9][10], where the periodic protein layers on both sides of the PG have been formed by the reattached subunits [81.…”