Ultrastructure of the human exocrine pancreas

Ekholm, R.; Edlund, Y

doi:10.1016/s0022-5320(59)90006-1

Cited by 70 publications

(22 citation statements)

References 18 publications

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“…Its general features are fairly constant for all mammalian species so far studied, i.e., the mouse (23-25), rat (26), guinea pig (I), cat (32), and man (27).…”

Section: B Morphologymentioning

confidence: 99%

Protein Synthesis, Storage, and Discharge in the Pancreatic Exocrine Cell

Lucien

Palade

1964

The Journal of Cell Biology

765

203

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The synthesis, intracellular transport, storage, and discharge of secretory proteins in and from the pancreatic exocrine cell of the guinea pig were studied by light-and electron microscopical autoradiography using DL-leucine-4,5-H 3 as label. Control experiments were carried out to determine: (a) the length of the label pulse in the blood and tissue after intravenous injections of leucine-H3; (b) the amount and nature of label lost during tissue fixation, dehydration, and embedding. The results indicate that leucine-H 3 can be used as a label for newly synthesized secretory proteins and as a tracer for their intracellular movements. The autoradiographic observations show that, at ~5 minutes after injection, the label is localized mostly in cell regions occupied by rough surfaced elements of the endoplasmic reticulum; at ~20 minutes, it appears in elements of the Golgi complex; and after 1 hour, in zymogen granules. The evidence conclusively shows that the zymogen granules are formed in the Golgi region by a progressive concentration of secretory products within large condensing vacuoles. The findings are compatible with an early transfer of label from the rough surfaced endoplasmic reticulum to the Golgi complex, and suggest the existence of two distinct steps in the transit of secretory proteins through the latter. The first is connected with small, smooth surfaced vesicles situated at the periphery of the complex, and the second with centrally located condensing vacuoles.

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“…Its general features are fairly constant for all mammalian species so far studied, i.e., the mouse (23-25), rat (26), guinea pig (I), cat (32), and man (27).…”

Section: B Morphologymentioning

confidence: 99%

Protein Synthesis, Storage, and Discharge in the Pancreatic Exocrine Cell

Lucien

Palade

1964

The Journal of Cell Biology

765

203

View full text Add to dashboard Cite

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“…This applies particularly to the basal membranes which follow a course around each acinus and continue on to the corresponding intercalary duct, making each such unit a morphologically well-defined entity. Ekholm and Edlund (1959) also report that part of the interacinar space which lies between the basement membranes forms a single interconnected system in each lobule. Those capillaries whose basement membrane provide the endothelium with a boundary structurally like that of the acinus pass through this space.…”

Section: Electron Microscopy Of Acinusmentioning

confidence: 89%

“…Apparently two different kinds of cells are distinguishable, the proportion varying from one duct to another. While one cell is essentially identical to the centroacinar cells, the other type contains a cytoplasm significantly greater in density than that of the centroacinar cell (Ekholm and Edlund, 1959). The free surface of the smallest excretory ducts shows microvilli protruding into the lumen of the duct.…”

Section: Ultrastructurementioning

confidence: 97%

“…Ekholm and Edlund (1959) report that the acini of the human exocrine pancreas are separated from one another by an interacinar space containing two basement membranes, each belonging to an acinus.…”

Section: Electron Microscopy Of Acinusmentioning

confidence: 99%

“…Those capillaries whose basement membrane provide the endothelium with a boundary structurally like that of the acinus pass through this space. The entire interacinar space is, therefore, regarded as a means for transport between the cells and the blood system (Ekholm and Edlund, 1959).…”

Section: Electron Microscopy Of Acinusmentioning

confidence: 99%

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