Ultrastructure of the uterine epithelium of mice treated neonatally with estrogen

Mori, Takao

doi:10.1159/000144871

Cited by 6 publications

(2 citation statements)

References 8 publications

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“…Immediate effects of estrogens on the developing uterus include qualitative and quantitative changes in protein synthesis (Newbold et al, 1981(Newbold et al, , 1984 and stimulation of epithelial proliferation (Forsberg, 1970;Eide, 1975a,b;Bigsby and Cunha, 1986). In adult mice treated neonatally with estrogen, the uterine epithelium may ex-Q 1989 ALAN R. LISS, INC press cystic hyperplasia (Takasugi et al, 1970;Yasuda et al, 1977;Rustia and Shubik, 1976;McLachlan et al, 1980;McLachlan and Fabro, 1982;Wordinger and Morrill, 1985) and/or squamous metaplasia (Dunn and Green, 1963;Takasugi and Bern, 1964;Rustia and Shubik, 1976;Yasuda et al, 1977;Mori, 1977;Vorherr et al, 1979;Baggs et al, 1980;Ennis and Davies, 1982;Wong et al, 1982;Walker 1980Walker , 1983Ostrander et al 1985). These proliferative lesions may progress to uterine adenocarcinoma (Rustia and Shubik, 1976;Napalkov and Anisimov, 1979;Vorherr et al, 1979;McLachlan et al, 1980;Gilloteaux et al, 1982;Walker, 1983).…”

Section: Introductionmentioning

confidence: 99%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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The effects of diethylstilbestrol (DES) treatment on myometrial development from the prenatal to adult period were examined in rats and mice by histologic and immunocytochemical methods using anti-actin, -vimentin, and -laminin to assess cytodifferentiation of smooth muscle and fibroblastic cells, and by morphometric procedures to assess quantitatively the effect of DES on the expression of cellular orientation in the emerging inner circular myometrial layer. Neonatal rats and mice were treated with DES from day 0 (day of birth) to day 2 with dosages known to perturb myometrial development. Neonatal treatment with DES increased the degree of circular orientation within the uterine mesenchyme, an effect detectable following as little as 24 hr of DES treatment. This effect on spatial organization of the mesenchyme was followed by an increase in the thickness of the actin-positive middle layer (prospective circular myometrium) of uterine mesenchyme during days 3-15; from day 15 onward, however, the circular myometrial layer began to fragment into irregular bundles of smooth muscle, and the longitudinal myometrial layer became thinner and more irregularly organized than controls. Vimentin localization in rats treated with DES neonatally was more intense than in controls within the circularly orientated uterine mesenchyme at 5 days. By 60 days the circular and longitudinal myometrial layers of DES-treated animals showed strands and bundles of vimentin-positive cells, which were not present in controls. Both rats and mice show comparable effects of DES treatment.

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Section: Introductionmentioning

confidence: 99%

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Brody

Cunha

1989

Am. J. Anat.

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“…ADL appeared in mice at a high incidence following neonatal treatment with DES (12,14), but seldom occurred in mice exposed prenatally to DES (1 6, 18). Abnormal uterine changes were observed in mice given injections of natural and synthetic estrogens or androgens during the neonatal period (19)(20)(21), but none of the previous studies demonstrated uterine changes at early postnatal ages in mice exposed prenatally to sex hormones, The present study, therefore, was aimed at examining the vaginal and uterine responses to prenatally and neonatally administered DES.…”

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confidence: 98%

Development of Vaginal Adenosis-like Lesions and Uterine Epithelial Stratification in Mice Exposed Perinatally to Diethylstilbestrol

Iguchi

Takase

Takasugi

1986

Experimental Biology and Medicine

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Abstracf. Pregnant ICR/JCL mice were given either four daily subcutaneous injections of 20-2000 pg diethylstilbestrol (DES)/day or a 4-day intravenous infusion of 20-200 pg DES/day starting on Day 15 of gestation. Female mice were injected with 0.01-50 pg DES/day for 5 days starting on the day of birth. Females given oil injections during the neonatal period and offspring of mothers given injections of an infusion of respective vehicles alone served as controls when corresponding ages were attained. Incidence of adenosis-like lesions (ADL) in the vaginal epithelium and stratification of the uterine epithelium (UST) were determined in both offspring and neonatally exposed mice at 30 days of age. Injections of 0.1-50 pg DES/day during the neonatal period induced ADL in the fornical epithelium of vagina (36-70%), but not UST. A high incidence of ADL (80-8870) was found in the fornical and upper-vaginal epithelia in offspring of mothers given injections of 200-2000 pg DES/day, and UST was encountered in 38-70% of these mice. Offspring of mothers given an infusion of 20 pg DES/day had low incidences of ADL ( 1 1%) and UST (22%). whereas offspring of mothers infused with 200 pg DES/day exhibited high incidences of ADL (71%) and UST (86%). In offspring of mothers given injections of 2000 pg DES/day, ADL appeared in the fornical and upper-vaginal epithelia as early as I5 days of age. Thus, the present study revealed that ADL and UST occur at a high incidence in infantile mice exposed prenatally to high doses of DES. Previous studies failed to detect such effects perhaps due to the strain of mice, methods, duration. and/or dose of DES used. G 1986 Society for Experimental Biology and Medicine. 59

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Effects of Steroid Hormones in the Neonate

Kincl

1990

Monographs on Endocrinology

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Ultrastructure of the uterine epithelium of mice treated neonatally with estrogen

Cited by 6 publications

References 8 publications

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Histologic, morphometric, and immunocytochemical analysis of myometrial development in rats and mice: II. Effects of DES on development

Development of Vaginal Adenosis-like Lesions and Uterine Epithelial Stratification in Mice Exposed Perinatally to Diethylstilbestrol

Effects of Steroid Hormones in the Neonate

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