Ultrastructure of type 1 and type 2 herpes simplex virus (HSV) infection of skin in genital regions

Boddingius, J.; Dijkman, H.; Joost, Th. van; Meijden, Willem van der; Schift, R.; Stolz, Erwin

doi:10.1016/0304-3991(84)90163-3

Cited by 2 publications

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“…Measurements of diameters of eore (nucleoid) and whole capsid of intranuclear HSV-2 were performed on capsids photographed at the highest feasible electron microscope magnification. Data were eompared with previous measurements of diameters of core and whole capsids of intranuclear HSV-1 (11,18). For calculations of exact magnifications of electron micrographs, a referenee grid was photographed (Fulham 1002x, shadow cast crossed lines carbon grating replica; 2160 lines per mm).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies on human herpes simplex infection have shown that, beside subtyping by cell culture, it is possible to distinguish between HSV-1 and HSV-2 infection by measuring core (nucleoid) diameters in electron micrographs (II,18). This, together with findings presented in this paper of HSV-2 capsids occurring within cells of the host's immune defence system, might encourage further EM studies of a) vehicles for viral dissemination and b) possible relationships between HSV infections and other diseases, including cancer.…”

Section: Hsv-2 In Phagocytic and Lymphoeytie Cellsmentioning

confidence: 99%

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HSV‐2 replication sites, monocyte and lymphocytic cell infection and virion phagocytosis by neutrophils, in vesicular lesions on penile skin

et al. 1987

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From a heterosexual male with recurrent genital herpes simplex virus (HSV-2) infection, a fresh intraepidermal vesicle on the penile skin was excised by punch biopsy, fixed and processed for electron microscopy. Differing locations and appearances of capsids and virions were studied to elucidate true host or destroyer cells. HSV-2 propagation and virion formation occurred predominantly in multi- or mononucleate spinosum cells situated at the base of the vesicle. However, some of the monocytes, young histiocytes and lymphocytic cells floating in the vesicle fluid were also involved. They harbored a small number of intranuclear capsids, designating the cells as viral (capsid) carriers. Infrequently encountered free virions in the vesicle fluid were invariably seen near neutrophils. All neutrophil granulocytes examined lacked intranuclear capsids. In contrast, distinct evidence of phagocytosis of virions and some capsids by neutrophils was found in the vesicle fluid near apical portions of spinosum cells packed with virions, or in neutrophils located between virion-loaded spinosum cells in the base lining of the vesicle. In the cytoplasm of neutrophils, single and lysosome-enclosed clusters of virions were noted. Myelin figures and vacuolation of lysosomes in free-floating neutrophils were suggestive of virion distintegration. Viral propagation and abundant virion formation, beside neutrophil and lymphocyte attack, eventually lead to spinosum cell destruction. The minimal cytopathic effects (CPE) observed in involved monocytes and lymphocytic cells floating in the vesicle fluid suggest that these cells might function as vehicles for HSV-2 (capsid) transport to the exterior or interior.

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Section: Methodsmentioning

confidence: 99%

Section: Hsv-2 In Phagocytic and Lymphoeytie Cellsmentioning

confidence: 99%

HSV‐2 replication sites, monocyte and lymphocytic cell infection and virion phagocytosis by neutrophils, in vesicular lesions on penile skin

et al. 1987

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Replication characteristics and core size of intranuclear herpes simplex virus (HSV-1) in genital skin lesions: Electronmicroscopy studies of a biopsy from a female patient

Boddingius¹,

Dijkman²,

Meijden³

et al. 1987

Journal of Medical Microbiology

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Summary. Herpes simplex virus (HSV) type 1 genital infection, leading to ulcerating lesions in a female patient, was studied by electronmicroscopy. Infection had probably been recent, through oro-genital contact with a cold sore on the husband's lip. Cellculture typing and serological tests indicated that the patient currently had an HSV-1 secondary infection. Aspects studied in a skin biopsy from an ulcerating labium majus were epidermal cell types infected, stages in virus genesis, virus core diameter in intranuclear capsids and extracellular appearance of virus. Different stages in virus genesis, in virus envelope formation and in nuclear and cytoplasmic degeneration were observed in the few remaining, rounded and swollen, epidermal (?) spinosum cells. Their nuclei, some with marginated chromatin, harboured besides dense-cored or empty capsids, electron-dense blobs possibly representing clones of immature virus and falling apart into aggregates of small granules. In other nuclei, large clusters of dense-cored capsids, some distinctly hexagonal in shape, had accumulated in wide gaps in the nuclear membrane whereas remaining nuclear membrane portions were quadruple and often engaged in viral envelope formation. Partially enveloped capsids and naked dense-cored capsids were seen extracellularly indicating their survival outside cells. An occasional virion was present in dermal blood vessel lumina. Measurements of the electron-dense core (nucleoid) of intranuclear capsids in electronmicrographs showed that the HSV-1 core diameter differs very significantly from the core of intranuclear HSV-2 capsids, thus allowing a clear distinction by electronmicroscopy between the two HSV subtypes in plastic-embedded biopsies.

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Ultrastructure of type 1 and type 2 herpes simplex virus (HSV) infection of skin in genital regions

Cited by 2 publications

References 0 publications

HSV‐2 replication sites, monocyte and lymphocytic cell infection and virion phagocytosis by neutrophils, in vesicular lesions on penile skin

HSV‐2 replication sites, monocyte and lymphocytic cell infection and virion phagocytosis by neutrophils, in vesicular lesions on penile skin

Replication characteristics and core size of intranuclear herpes simplex virus (HSV-1) in genital skin lesions: Electronmicroscopy studies of a biopsy from a female patient

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