Ultrathin Coatings with Change in Reactivity over Time Enable Functional In Vitro Networks Of Insect Neurons

Reska, Anna; Gasteier, Peter; Schulte, Petra; Moeller, Martin; Offenhäusser, Andreas; Groll, Jürgen

doi:10.1002/adma.200800270

Cited by 22 publications

(14 citation statements)

References 25 publications

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“…Fibers were modified with peptide sequences derived from fibronectin (GRGDS), and collagen IV (GEFYFDLRLKGDK), two of the major constituents of the ECM 21. Immobilization of different peptide sequences and other active molecules containing an amino group has been reported in detail previously 22, 23. Briefly, in the case of the NCO‐ s P[EO‐ co ‐PO]/PLGA fibers, the free isocyanate groups are used for peptide immobilization.…”

Section: Resultsmentioning

confidence: 99%

Electrospun, Biofunctionalized Fibers as Tailored in vitro Substrates for Keratinocyte Cell Culture

Grafahrend

Heffels

Möller

et al. 2010

Macromolecular Bioscience

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Cell adhesion preventing fiber surfaces were tailored differently with bioactive peptides (a fibronectin fragment (GRGDS), a collagen IV fragment (GEFYFDLRLKGDK) and a combination of both) to provide an artificial extracellular matrix as a substrate for HaCaT keratinocyte cell culture. Therefore, a polymer blend containing a six-arm star-shaped statistical copolymer of ethylene oxide and propylene oxide in the ratio 80:20 (NCO-sP[EO-co-PO]) and poly-[D,L-(lactide-co-glycolide)] (PLGA) was electrospun. The resulting fibers were biofunctionalized and investigated as in vitro substrates using the HaCaT kerationcyte cell line. Appropriate surface chemistry on these electrospun fibers proved to prevent adhesion of keratinocytes, while additional immobilization of certain peptide sequences induced cell adhesion. These specific fibers enable investigation of immobilized active molecules and the subsequent cellular response to the scaffold. HaCaT keratinocytes were found to selectively adhere to those fibers modified with either collagen IV segment GEFYFDLRLKGDK or a mixture of the two peptide sequences GEFYFDLRLKGDK and GRGDS (1:1). However, the synergistic effects of both (the fibronectin fragment and the collagen IV fragment) seem to significantly increase the numbers of adherent keratinocytes.

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Section: Resultsmentioning

confidence: 99%

Electrospun, Biofunctionalized Fibers as Tailored in vitro Substrates for Keratinocyte Cell Culture

Grafahrend

Heffels

Möller

et al. 2010

Macromolecular Bioscience

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“…On the other hand, there is renewed interest in exploiting neurons as biosensing devices because they use electrical signals for task-related information processing. [22,23] In most of cases, neuron-silicon junctions are created by attaching the cells to the substrate by using Con A as an adhesive. [24] In this context, the incorporation of electroactive centers into the Con A layer would introduce a new variable to modulate the electrical communication between the cells and the transistor.…”

Section: à2mentioning

confidence: 99%

Facile Glycoenzyme Wiring to Electrode Supports by Redox‐Active Biosupramolecular Glue

Pallarola

Queraltó

Knoll

et al. 2010

Chemistry A European J

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“…However, culturing neurons within microfluidic chips over long terms is a challenge, possibly due to limited nutrient exchange. Surface chemistry [37][38][39] allowed elegantly patterned neuronal networks with potentially useful functions, but long term maintenance of the networks is a problem. SAMs [40,41] represent a convenient way for engineering cell adhesion [42], as SAMs do not involve complicated instruments during the experiment.…”

Section: Introductionmentioning

confidence: 99%

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

et al. 2012

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By quantitatively comparing a variety of macromolecular surface coating agents, we discovered that surface coating strongly modulates the adhesion and morphogenesis of primary hippocampal neurons and serves as a switch of somata clustering and neurite fasciculation in vitro. The kinetics of neuronal adhesion on poly-lysine-coated surfaces is much faster than that on laminin and Matrigel-coated surfaces, and the distribution of adhesion is more homogenous on poly-lysine. Matrigel and laminin, on the other hand, facilitate neuritogenesis more than poly-lysine does. Eventually, on Matrigel-coated surfaces of self-assembled monolayers, neurons tend to undergo somata clustering and neurite fasciculation. By replacing coating proteins with cerebral astrocytes, and patterning neurons on astrocytes through self-assembled monolayers, microfluidics and micro-contact printing, we found that astrocyte promotes soma adhesion and astrocyte processes guide neurites. There, astrocytes could be a versatile substrate in engineering neuronal networks in vitro. Besides, quantitative measurements of cellular responses on various coatings would be valuable information for the neurobiology community in the choice of the most appropriate coating strategy.

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Ultrathin Coatings with Change in Reactivity over Time Enable Functional In Vitro Networks Of Insect Neurons

Cited by 22 publications

References 25 publications

Electrospun, Biofunctionalized Fibers as Tailored in vitro Substrates for Keratinocyte Cell Culture

Electrospun, Biofunctionalized Fibers as Tailored in vitro Substrates for Keratinocyte Cell Culture

Facile Glycoenzyme Wiring to Electrode Supports by Redox‐Active Biosupramolecular Glue

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

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