Ultraviolet-Absorption Spectra of Dry Bacterial Spores

Bailey, Glen F.; Karp, Saima; Sacks, L. E.

doi:10.1128/jb.89.4.984-987.1965

Cited by 74 publications

(42 citation statements)

References 9 publications

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“…Another possibility is that the presence of other constituents such as dipicolinic acid in the vicinity of DNA may affect the spectra by facilitating energy transfer in the unique environment of the spore core. This is supported by the fact that dipicolinic acid exhibits a closely parallel pattern of absorption spectrum in this wavelength region (Bailey et al, 1965). The amount or distribution of this material may be somewhat different in the recombination-defective spores, particularly in RCE spores.…”

Section: Discussionmentioning

confidence: 84%

INACTIVATION ACTION SPECTRA OF BACILLUS SUBTILIS SPORES IN EXTENDED ULTRAVIOLET WAVELENGTHS (50–300nm) OBTAINED WITH SYNCHROTRON RADIATION

Munakata¹,

Saito

Hieda

1991

Photochem & Photobiology

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Five types of Bacillus subtilis spores (UVR, UVS, UVP, RCE, and RCF) differing in repair and/or recombinational capabilities were exposed to monochromatic radiations at 13 wavelengths from 50 to 300 nm in vacuum. An improved biological irradiation system connected to a synchrotron radiation source was used to produce monochromatic UV radiation in this extended wavelength range with sufficient fluence to inactivate bacterial spores. From the survival curves obtained, the action spectra for the inactivation of the spores were depicted. Recombination-deficient RCE (recE) and RCF (recF) spores were more sensitive than the wild-type UVR spores in the entire range of wavelengths. This was considered to mean that DNA was the major target for the inactivation of the spores. Vacuum-UV radiations of 125-175 nm were effective in killing the spores, and distinct peaks of the sensitivity were seen with all types of the spores. Insensitivities at 190 and 100 nm were common to all five types of spores, indicating that these wavelengths were particularly impenetrant and absorbed by the outer layer materials. The vacuum-UV peaks centering at 150 nm were prominent in the spores defective in recombinational repair, while the far-UV peaks at around 235 and 270 nm were prominent in the UVS (uvrA ssp) and UVP (uvrA ssp polA) spores deficient in removal mechanisms of spore photoproducts. Thus, the profiles of the action spectra were explained by three factors; the penetration depth of each radiation in a spore, the efficiency of producing DNA damage that could cause inactivation, and the repair capacity of each type of spore.

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Section: Discussionmentioning

confidence: 84%

INACTIVATION ACTION SPECTRA OF BACILLUS SUBTILIS SPORES IN EXTENDED ULTRAVIOLET WAVELENGTHS (50–300nm) OBTAINED WITH SYNCHROTRON RADIATION

Munakata¹,

Saito

Hieda

1991

Photochem & Photobiology

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“…These results suggest strongly that Ca in the core is bound with DPA if the assumption is accepted that DPA is associated with Ca (4,24,33,34). In other words, it means that DPA is concentrated in the core as the cryptobiotic state of Ca-DPA.…”

Section: Germinated Sporementioning

confidence: 78%

“…The bacterial spore is a microorganism unique in structure and chemical composition as well as in physiological properties. Among the chemical components of the spore, Ca was detected as 1 to 5% of the spore dry weight (6,29), suggesting that it is present mostly in association with dipicolinic acid (DPA) (4,24,33,34), and it has been suggested and thoroughly discussed that Ca plays an important role in spore properties such as high resistance, dormancy, cryptobiosis and germinability (1-3, 7, 8, 19, 21, 22, 26, 27, 32). Considering its role in these mechanisms, the location of Ca is an important factor, and many have suggested that the location of Ca is in the coat, the cortex, or the core (9, 10, 16-21, 25, 28, 30, 31), but there is no definite and direct evidence for these locations.…”

mentioning

confidence: 99%

Location of Elements in Ashed Spores of Bacillus megaterium

Nishihara

Ichikawa

Kondo

1980

Microbiology and Immunology

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The structure of the skeleton of spores of Bacillus megaterium was examined after ashing in a plasma asher and the elemental composition of the ashed whole spores was determined with an analytical electron microscope. All spores were ashed in situ although they shrank by about 15%. Even P and S, in addition to metals, were recovered well from ashed samples. Ash was rich in the core and the coat, and poor in the cortex. Ca, P, S, and Mg were detected in the core and coat of the spore of B. megaterium QM B 1551. Ca in the core was markedly decreased by germination or autoclaving. In the spore of B. megaterium ATCC 19213, almost all of the ash was detected in the core and its elemental composition was similar to that of the core of the strain QM B1551 spore.These results suggest strongly that the core is the site of Ca associated with dipicolinic acid.

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“…The distribution of DPA and the occurrence of DPA conjugates in the spore have yet to be substantiated, but it is possible that several forms or states of DPA exist in the spore and are located in the cytoplasm or cortex rather than in the spore coat or exosporium (Halvorson and How&, 1961). Bailey et al (1965) have presented ultraviolet absorption spectra of dry bacterial spores that suggest that DPA does exist largely as a calcium chelate in the spore. Windle and Sacks (1963) have found evidence for bonding of copper with protein and for two different types of bonding for manganese in spores, which could lead to speculation on the role of various metallic cations on structure and stability of the spore.…”

Section: And Discussionmentioning

confidence: 99%

Release of Cellular Constituents During Heat Inactivation of Endospores of Aerobic Bacilli

Walker

Matches

1965

Journal of Food Science

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SUMMARY Release of dipicolinic acid from spores of some species of Bacillus during heating at 100°C was determined. The total amount and rate of release of dipicolinic acid from these spores varied between species and strains, the most resistant spores releasing the least. Studies were made on Bacillus polymyxa and B. megaterium to determine the influence of buffers and pH on the liberation of dipicolinic acid, carbohydrate, and nitrogen. Release of these materials was usually least at pH 7.0 and greatest under conditions that favored thermal inactivation. Some of the amino acids and carbohydrates released were identified.

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Ultraviolet-Absorption Spectra of Dry Bacterial Spores

Cited by 74 publications

References 9 publications

INACTIVATION ACTION SPECTRA OF BACILLUS SUBTILIS SPORES IN EXTENDED ULTRAVIOLET WAVELENGTHS (50–300nm) OBTAINED WITH SYNCHROTRON RADIATION

INACTIVATION ACTION SPECTRA OF BACILLUS SUBTILIS SPORES IN EXTENDED ULTRAVIOLET WAVELENGTHS (50–300nm) OBTAINED WITH SYNCHROTRON RADIATION

Location of Elements in Ashed Spores of Bacillus megaterium

Release of Cellular Constituents During Heat Inactivation of Endospores of Aerobic Bacilli

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