Ultraviolet Excitation Photothermal Spectroscopy of Non-Labeled Amino Acids and Visible Light-Induced Signal Enhancement

Hirashima, Satoshi; Harata, Akira

doi:10.1143/jjap.47.3970

Cited by 7 publications

(4 citation statements)

References 17 publications

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“…For example, reversed-phase mode has versatility for general organic molecules and normal-phase mode could enhance the sensitivity further using organic solvents. Moreover, DIC-TLM can detect biomolecules such as proteins and nucleic acids without fluorescent labeling if its excitation wavelength is tuned to UV region [23,24]. Therefore, the application of DIC-TLM to extended nanochromatography is a giant step for bioanalysis on chip device.…”

Section: Discussionmentioning

confidence: 99%

Detection of nonfluorescent molecules using differential interference contrast thermal lens microscope for extended nanochannel chromatography

Shimizu

Mawatari

Kitamori

2011

J of Separation Science

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An ultrasensitive absorbance detector, the differential interference contrast thermal lens microscope (DIC-TLM), was employed for a chromatography system using silica nanochannel. Recently, separation of ultrasmall volume sample has been strongly required for single-cell biological and chemical analysis. Previously, we have developed a chromatography system using nanochannels of ~100 nm scale (extended nanochannels) fabricated on a silica substrate. The extended nanochromatography realized highly efficient separation of samples <1 fL without packing materials. However, its detection method was limited to fluorescence method due to the small volume, and a new detector based on absorbance has been required. On the contrary, we have also developed DIC-TLM, a photothermal spectrometer based on absorption and thermal relaxation of sample for determination of concentration of nonfluorescent molecules in extended nanochannel. In this paper, we combined the extended nanochromatography and the DIC-TLM for separation and detection of nonfluorescent dyes. Particularly, basic performances of the DIC-TLM including quantitative performance and sensitivity were deliberated for injected samples of ~fL volume.

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Section: Discussionmentioning

confidence: 99%

Detection of nonfluorescent molecules using differential interference contrast thermal lens microscope for extended nanochannel chromatography

Shimizu

Mawatari

Kitamori

2011

J of Separation Science

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“…55) As mentioned above, ultrasound interacts with light, and various measurement methods, devices, and applications based on this principle have been researched, forming a research field called acousto-optics. 30,31,34,39,40,[56][57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75] Brillouin scattering is caused when light is incident to a medium in which an ultrasonic wave propagates and the light and the ultrasonic wave interact with each other. This phenomenon itself is interesting and is also utilized to measure material properties such as elasticity and so forth.…”

Section: Ultrasonic Properties Of Materials Phonon Physics Acousto-op...mentioning

confidence: 99%

Introduction of measurement techniques in ultrasonic electronics: Basic principles and recent trends

Mizutani¹,

Wakatsuki²,

Ebihara³

2016

Jpn. J. Appl. Phys.

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Measurementthe act of measuring physical properties that we performhas the potential to contribute to the successful advancement of sciences and society. To open doors in physics and other sciences, various measurement methods and related applications have been developed, and ultrasound has remained a useful probe, power source, and interesting measurement object for the past two centuries. In this paper, we first summarize the basic principles of ultrasound from the viewpoint of measurement techniques for readers who just have started studying or are interested in the field of ultrasonic electronics. Moreover, we also introduce recent studiesultrasonic properties of materials, measurement techniques, piezoelectric devices, nonlinear acoustics, biomedical ultrasound, and ocean acousticsand their trends related to measurement techniques in ultrasonic electronics to provide some ideas for related applications.

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“…To detect non-labeled amino acids in solution by TL measurements, excitation with an UV laser is essential. Recently, our group has successfully detected non-labeled amino acids with a home-built UV-excitation TL detection system using an UV-excitation beam at a wavelength ranging from 212 to 220 nm [8].…”

Section: Introductionmentioning

confidence: 99%

Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing

Katae

Hirashima

Harata

2010

J. Phys.: Conf. Ser.

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Abstract. Thermal lensing (TL) permits ultrasensitive measurements of optical absorption of analytes in very small volumes. Separation-detection conditions of non-labeled amino acids with micro-HPLC/UV absorption detector are optimized, and direct determination of nonlabeled amino acids by micro-HPLC/UV-excitation TL detection in a gradient elution is successfully demonstrated. Non-labeled amol-level amino acids is detectable with the TL detection system, which has thousand times better sensitivity than a conventional UV detector.

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Ultraviolet Excitation Photothermal Spectroscopy of Non-Labeled Amino Acids and Visible Light-Induced Signal Enhancement

Cited by 7 publications

References 17 publications

Detection of nonfluorescent molecules using differential interference contrast thermal lens microscope for extended nanochannel chromatography

Detection of nonfluorescent molecules using differential interference contrast thermal lens microscope for extended nanochannel chromatography

Introduction of measurement techniques in ultrasonic electronics: Basic principles and recent trends

Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing

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