Ultraviolet Photodissociation Induced by Light‐Emitting Diodes in a Planar Ion Trap

Abstract

The first application of light-emitting diodes (LEDs) for ultraviolet photodissociation (UVPD) mass spectrometry is reported. LEDs provide a compact, low cost light source and have been incorporated directly into the trapping cell of an Orbitrap mass spectrometer. MS/MS efficiencies of over 50 % were obtained using an extended irradiation period, and UVPD was optimized by modulating the ion trapping parameters to maximize the overlap between the ion cloud and the irradiation volume.

Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules

“…A variety of light sources have been used for UVPD, including excimer lasers (e.g., F 2 : 157 nm, 7.9 eV per photon; ArF: 193 nm, 6.4 eV per photon; and XeF: 351 nm, 3.5 eV per photon), solid state Nd:YAG lasers (213 nm, 5.8 eV per photon; 266 nm, 4.7 eV per photon; or 355 nm, 3.5 eV per photon), gas-discharge lamps, synchrotron radiation, and even light-emitting diodes (LEDs) in one report . More powerful lasers or ones with high repetition rates enable UVPD on a fast time scale and are particularly beneficial for higher-throughput LC-MS applications.…”

PITTCON‐Preise 2020

“…Brodbelts Gruppe beschäftigt sich mit Photodissoziations‐Massenspektrometrie zur Charakterisierung biologischer Moleküle. In einer Angewandte‐Chemie ‐Zuschrift beschrieben sie die Photodissoziation durch Ultraviolett‐Leuchtdioden in einer ebenen Ionenfalle …”

PITTCON Awards 2020

“…Research in the Brodbelt group is focusd on the development of photodissociation mass spectrometry for characterization of biological molecules.I naCommunication in Angewandte Chemie they described an ultraviolet photodissociation induced by light-emitting diodes in ap lanar ion trap. [5] AndrewJ . Armstrong investigates the theory,m echanism, and uses of chiral molecular recognition.…”