Ultraviolet radiation as a ballast water treatment strategy: Inactivation of phytoplankton measured with flow cytometry

Olsen, Ranveig Ottoey; Hoffmann, Friederike; Hess-Erga, Ole-Kristian; Larsen, Aud; Thuestad, Gunnar; Hoell, Ingunn Alne

doi:10.1016/j.marpolbul.2015.12.008

Cited by 38 publications

(26 citation statements)

References 47 publications

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“…The lowest UV dose is comparable to those used in BWTS, but ballast water is treated twice (on uptake and discharge). UV doses between 400 and 800 mJ cm −2 were not applied, since previous FCM studies showed that CFDA-AM-stained T. suecica UV irradiated with 400 mJ cm −2 were permanently inactivated (Olsen et al 2016). Doses of 400 and 800 mJ cm −2 were both included in the study to examine whether and how esterase activity and membrane integrity differed in these samples.…”

Section: Experimental Set-upmentioning

confidence: 99%

Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality

Olsen¹,

Hess-Erga²,

Larsen³

et al. 2016

Aquat. Biol.

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UV] irradiation, heat, and cavitation) treatment technologies (Shannon et al. 2008, Werschkun et al. 2012, 2014). Traditionally, water analysis used to assess most treatment technologies has depended on cultivation, such as the plate count or the most probable number (MPN) technique. Cultivation methods measure viability of organisms present, i.e. the ability of a cell to reproduce. Vital (live) cells can be either viable or non-viable, whereas non-vital (dead)

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Section: Experimental Set-upmentioning

confidence: 99%

Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality

Olsen¹,

Hess-Erga²,

Larsen³

et al. 2016

Aquat. Biol.

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“…Many research studies have been undertaken in the search for effective approaches to treat contaminated water. Mori et al [6] and Olsen et al [7] suggested an ultra violet lightemitting diode (UV-LED) device as an effective method to sterilize water. Sinclair et al [8] conducted water sterilization using cellulose membrane filters grafted with silver nanoparticles and nanowires.…”

Section: Introductionmentioning

confidence: 99%

Water electrode plasma discharge to enhance the bacterial inactivation in water

Hwang

Jeong

You

et al. 2017

Biotechnology & Biotechnological Equipment

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The present study used water-electrode plasma discharge to increase the effect of bacterial inactivation in water for bioengineering and biotechnological applications. The water-electrode plasma discharge system was fabricated using a newly designed plasma generator and a highvoltage power supply. Water contaminated with Escherichia coli was treated with water-electrode plasma discharge for 0, 1, 2, 3, 4, 5, 10, 20 and 30 min. As a result, the colony-forming units (cfu) of E. coli were reduced with plasma treatment time, reaching nearly complete inactivation after 30 min. In addition, rapid generation of H 2 O 2 in the contaminated water was observed, which could mainly account for the effective bacterial inactivation. In conclusion, direct generation of reactive chemical species under water was successfully achieved by using a water-electrode plasma discharge system, which could be practically used to enhance bacterial inactivation in a variety of bioengineering applications. KEYWORDSWater electrode; plasma discharge; bacterial inactivation; hydrogen peroxide CONTACT Jinmu Jung jmjung@jbnu.ac.kr * These authors contributed equally to this work.

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“…Similarly, the dose requirements depends on the treatment context, as it may require lower doses if the treatment is applied during the ballasting and then followed by a light-limited period, or higher doses if immediate impairment is necessary, in case of the treatment during the ballast discharge (Olsen et al 2016). The survivorship of phytoplankton organisms such as Tisochrysis lutea (formerly known as Isochrysis galbana, T-ISO; Bendif et al 2013) presents a clear response to UV irradiation, and their adequate growth rate, obvious degradation of nonviable organisms, ease and stability in culturing and wide commercial availability constitute a consistent basis for developing a normalized protocol to determine the inactivating effect of UV-based treatments.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of ultraviolet disinfection of microalgae by growth modeling: application to ballast water treatment

et al. 2016

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Ultraviolet disinfection is a frequent option for eliminating viable organisms in ballast water to fulfill international and national regulations. The objective of this work is to evaluate the reduction of microalgae able to reproduce after UV irradiation, based on their growth features. A monoculture of microalgae Tisochrysis lutea was irradiated with different ultraviolet doses (UV-C 254 nm) by a flow-through reactor. A replicate of each treated sample was held in the dark for 5 days simulating a treatment during the ballasting; another replicate was incubated directly under the light, corresponding to the treatment application during de-ballasting. Periodic measurements of cell density were taken in order to obtain the corresponding growth curves. Irradiated samples depicted a regrowth following a logistic curve in concordance with the applied UV dose. By modeling these curves, it is possible to obtain the initial concentration of organisms able to reproduce for each applied UV dose, thus obtaining the dose-survival profiles, needed to determine the disinfection kinetics. These dose-survival profiles enable detection of a synergic effect between the ultraviolet irradiation and a subsequent dark period; in this sense, the UV dose applied during the ballasting operation and subsequent dark storage exerts a strong influence on microalgae survival. The proposed methodology, based on growth modeling, established a framework for comparing the UV disinfection by different devices and technologies on target organisms. This procedure may also assist the understanding of the evolution of treated organisms in more complex assemblages such as those that exist in natural ballast water.

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Ultraviolet radiation as a ballast water treatment strategy: Inactivation of phytoplankton measured with flow cytometry

Cited by 38 publications

References 47 publications

Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality

Dual staining with CFDA-AM and SYTOX Blue in flow cytometry analysis of UV-irradiated Tetraselmis suecica to evaluate vitality

Water electrode plasma discharge to enhance the bacterial inactivation in water

Evaluation of ultraviolet disinfection of microalgae by growth modeling: application to ballast water treatment

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