Ultraviolet sensitivity and functional capacity in bacteriophage T4

Ebisuzaki, Kaney

doi:10.1016/0022-2836(66)90010-6

Cited by 13 publications

(9 citation statements)

References 24 publications

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“…The total measurement would include all three. Since v-induced excision repair appears to occur before replicative synthesis (26), one might expect the increased UV sensitivity of T4v DNA synthesis, as has been observed here and elsewhere (8). On the other hand, if excision repair is expanded in the absence of x-y recombinational repair, one might expect a decreased radiosensitivity of DNA synthesis for the x and y mutants, as was shown above.…”

Section: Resultssupporting

confidence: 60%

“…FIG. 8. Effect of rifampin on DNA synthesis measured by high-specific-activity (65 Ci/mmol) incorporation of33 ACi ofthymidine per ml (final concentration, 0.12 pg/ml) at 20°C.…”

Section: Resultsmentioning

confidence: 99%

“…Yet this same property also helps create a barrier to elucidating the T4 DNA synthetic events because of its intimate link with DNA synthesis. 8 and 15 min after infection with T4+, T4x, and T4y. Infected cells were incubated at 370C until 1 min before labeling, at which time they were switched to 20TC.…”

Section: Resultsmentioning

confidence: 99%

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Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

Melamede¹,

Wallace²

1977

J Virol

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The bacteriophage T4 recombination-deficient mutants x and y exhibited decreased rates of DNA synthesis as compared to wild-type T4. Mutant-induced DNA synthesis was more sensitive to mitomycin C than was wild-type synthesis. However, DNA synthesis in mutant-and wild-type-infected cells exhibited the same sensitivity to UV light and X-irradiation. When high-specific-activity label was administered at various times postinfection, mutant DNA synthesis resembled that of wild type for 12 min, after which time mutant-induced incorporation was greatly decreased and sensitive to mitomycin C as compared to that of the wild type. Rifampin and chloramphenicol studies indicated that the gene products necessary for synthesis measured at 15 min postinfection, including those of x+ and y+, were transcribed within 2 min and translated within 8 min postinfection. Administration of chloramphenicol to mutant xor mutant y-infected cells exactly 8 min postinfection, however, allowed for increased synthesis at 15 min that was sensitive to mitomycin C. Cells coinfected with T4+ and T4x or T4x and T4y retained a reduced mutant-type synthesis, whereas cells coinfected with T4+ and T4y exhibited a synthesis more closely resembling that of wild type.

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Section: Resultssupporting

confidence: 60%

“…FIG. 8. Effect of rifampin on DNA synthesis measured by high-specific-activity (65 Ci/mmol) incorporation of33 ACi ofthymidine per ml (final concentration, 0.12 pg/ml) at 20°C.…”

Section: Resultsmentioning

confidence: 99%

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Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

Melamede¹,

Wallace²

1977

J Virol

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“…Experimental results consistent with Ebisuzaki's (1966) hypothesis were provided by Maynard-Smith and Symonds (1973a). They showed that, when the repair of UV radiation damage to gene 42 (dCMP hydroxymethylase) was dependent on an excision mode, i.e.…”

Section: (C) Other T4 Genessupporting

confidence: 74%

“…An intriguing approach to an analysis of the role of T4 genes in the repair of UV damage stems from the studies of Ebisuzaki (1966) and Maynard-Smith and Symonds (1973a). The former author investigated quantitatively the effect of UV irradiation on the expression of a number of early and late phage-coded enzymes.…”

Section: (C) Other T4 Genesmentioning

confidence: 99%