Ulvan mediated VE cadherin antibody and REDV peptide co-modification to improve endothelialization potential of bioprosthetic heart valves

Liu, Xianbao; Yu, Kaixiang; Cheng, Si; Ren, Tanchen; Maitusong, Miribani; Liu, Feng; Chen, Jinyong; Qian, Yi; Xu, Dan; Zhu, Gangjie; Fang, Jun; Cao, Naifang; Wang, Jianan

doi:10.1016/j.msec.2021.112337

Cited by 14 publications

(9 citation statements)

References 53 publications

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“…Worth mentioning, cytokines such as SDF-1α, MCP1 and VEGF have also been used to promote mobilization and recruitment of EPCs, [37,40] while nanostructures and specific antibodies including CD34 and CD133 have been applied to capture and enhance EPCs adhesion on the scaffolds. [41][42][43] GDF11 takes advantage of being both specific and functional at the same time compared to above-mentioned methods, which is to say, GDF11 not only promoted recruitment and capture EPCs with certain specificity, but also improved the function of EPCs by inhibiting apoptosis and supporting cell growth. Currently, researches on GDF11 remained focused on its physiological and pathological functions, this study for the first time extended its usage in the field of biomaterials by applying to the construction of TEHV.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Generation and Endothelial Progenitor Cells Recruitment for Improving Hemocompatibility and Accelerating Endothelialization of Tissue Engineering Heart Valve

Zhou

Yan

Wen

et al. 2022

Adv Funct Materials

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Tissue engineering heart valve (TEHV) offers great potential to overcome the limitations of commercial artificial valves used in clinical practice as a permanent prosthetic valve. Currently, decellularized heart valve (DHV) is the most widely used scaffold for TEHV, but showed suboptimal performance due to difficulty of endothelialization. Facilitating endothelialization of DHV is indispensable for better valve performance, and excellent hemocompatibility guarantees enough time windows for endothelialization process. Herein, a dual‐functional TEHV scaffold with improving hemocompatibility and accelerating endothelialization is constructed by modifying DHV with copper ions (Cu) and growth differentiation factor 11 (GDF11). Results show the newly‐constructed scaffold successfully generates endogenous nitric oxide (NO) through catalysis of Cu, and possesses improved hemocompatibility by down‐regulating platelets activation and adhesion. Furthermore, GDF11 immobilization significantly accelerates scaffold endothelialization through facilitating recruitment, supporting growth, and alleviating apoptosis of endothelial progenitor cells . This TEHV scaffold shows favorable performance under in vivo hemodynamic environment with intact endothelial coverage and adaptive ECM remodeling, and without thrombus or calcification formation. This newly‐constructed TEHV scaffold is expected to make up for the shortcomings of currently available prosthetic valves in clinical practice and has the potential possibility of rapid translation to the clinic as a better prosthetic valve.

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Section: Discussionmentioning

confidence: 99%

Nitric Oxide Generation and Endothelial Progenitor Cells Recruitment for Improving Hemocompatibility and Accelerating Endothelialization of Tissue Engineering Heart Valve

Zhou

Yan

Wen

et al. 2022

Adv Funct Materials

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“…There are two main reasons for this: loss of activity and attraction of cell types other than ECs, such as platelets ( Zhao and Feng, 2020 ). Recently, the REDV peptide, which attracts ECs via recognition of the α4β1 integrin receptor expressed on the EC membrane, was reported as having high specificity, stability and efficiency for endothelialization ( Chen et al, 2018 ; Mahara et al, 2020 ; Liu X. et al, 2021 ; Liu Y. et al, 2021 ). Therefore, we designed a modification of ePTFE that combines heparin/collagen multilayers and REDV peptides to simultaneously reduce thrombosis formation and achieve in situ endothelialization.…”

Section: Discussionmentioning

confidence: 99%

Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues

Shan

Chen

Shi

et al. 2022

Front. Bioeng. Biotechnol.

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Prosthetic implants of expanded polytetrafluoroethylene (ePTFE) in the cardiovascular system have a high failure rate over the long term because of thrombosis and intimal hyperplasia. Although multiple surface modification methods have been applied to improve the anti-thrombotic and in situ endothelialization abilities of ePTFE, none have delivered outstanding results in vivo. Our previous study combined heparin/collagen multilayers and REDV peptides to modify ePTFE, and the in-vitro results showed that modification ePTFE with heparin/collagen-REDV can promote the cytocompatibility and antiplatelet property. This study illustrated the physical change, selective endothelial cells capture ability, and in vivo performance in further. The physical test demonstrated that this modification improved the hydrophilicity, flexibility and strength of ePTFE. A competition experiment of co-cultured endothelial cells and vascular smooth muscle cells verified that the heparin/collagen-REDV modification had high specificity for endothelial cell capture. A rabbit animal model was constructed to evaluate the in vivo performance of modified ePTFE implanted in the right ventricular outflow tract. The results showed that heparin/collagen-REDV modification was safe, promoted endothelialization, and successfully achieved regional anti-thrombosis without influencing body-wide coagulation function. The pathologic manifestations and mRNA expression pattern in tissues in contact with modified ePTFE indicated that this modification method may reduce M2-type macrophage infiltration and the expression of genes related to immune and inflammatory responses. The heparin/collagen-REDV modification may lower the incidence of complications related to ePTFE implantation and has good prospects for clinical use.

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“…In order to achieve improved cytocompatibility, mechanical properties, and biological function of decellularized scaffolds without inflammatory reactions, the surface modification could be done via various methods and biomaterials ( Ozasa et al, 2013 ; Liu et al, 2021b ). It is demonstrated that immersion of decellularized tendons with carbodiimide-derivatized hyaluronic acid and gelatin could alleviate the tendon’s gliding resistance by augmenting its surface’s smoothness ( Ozasa et al, 2013 ).…”

Section: Pre-application Processingmentioning

confidence: 99%

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

Neishabouri

Khaboushan

Daghigh

et al. 2022

Front. Bioeng. Biotechnol.

114

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Reproduction of different tissues using scaffolds and materials is a major element in regenerative medicine. The regeneration of whole organs with decellularized extracellular matrix (dECM) has remained a goal despite the use of these materials for different purposes. Recently, decellularization techniques have been widely used in producing scaffolds that are appropriate for regenerating damaged organs and may be able to overcome the shortage of donor organs. Decellularized ECM offers several advantages over synthetic compounds, including the preserved natural microenvironment features. Different decellularization methods have been developed, each of which is appropriate for removing cells from specific tissues under certain conditions. A variety of methods have been advanced for evaluating the decellularization process in terms of cell removal efficiency, tissue ultrastructure preservation, toxicity, biocompatibility, biodegradability, and mechanical resistance in order to enhance the efficacy of decellularization methods. Modification techniques improve the characteristics of decellularized scaffolds, making them available for the regeneration of damaged tissues. Moreover, modification of scaffolds makes them appropriate options for drug delivery, disease modeling, and improving stem cells growth and proliferation. However, considering different challenges in the way of decellularization methods and application of decellularized scaffolds, this field is constantly developing and progressively moving forward. This review has outlined recent decellularization and sterilization strategies, evaluation tests for efficient decellularization, materials processing, application, and challenges and future outlooks of decellularization in regenerative medicine and tissue engineering.

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Ulvan mediated VE cadherin antibody and REDV peptide co-modification to improve endothelialization potential of bioprosthetic heart valves

Cited by 14 publications

References 53 publications

Nitric Oxide Generation and Endothelial Progenitor Cells Recruitment for Improving Hemocompatibility and Accelerating Endothelialization of Tissue Engineering Heart Valve

Nitric Oxide Generation and Endothelial Progenitor Cells Recruitment for Improving Hemocompatibility and Accelerating Endothelialization of Tissue Engineering Heart Valve

Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues

Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods

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