Uma Análise Epistêmica Para a Elucidação Do Complexo De Espécies Crípticas

Allgayer, Heloísa; Hiller, Rafael Francisco; Valiati, Victor Hugo

doi:10.18226/21784612.v26.e021005

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Morphologically, P. ornatus presents with vegetative characteristics similar to other species of the genus Plectranthus (Lamiaceae), such as P. barbatus , P. neochilus [ 14 ], P. amboinicus [ 18 ], P. verticillatus [ 19 ], and P. neochilus [ 20 ]. This can also be one of the factors that can hinder the correct taxonomic identification of the species of the genus Plectranthus .…”

Section: Resultsmentioning

confidence: 99%

Morphoanatomical, Histochemical, and Essential Oil Composition of the Plectranthus ornatus Codd. (Lamiaceae)

da Silva,

Correia,

Gurgel

et al. 2023

Molecules

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Plectranthus ornatus is a medicinal and aromatic plant used in traditional and alternative medicine. In this study, leaves of P. ornatus were collected in two cities of the state of Pará, “Quatipuru” and “Barcarena”, and were used with the objective of analyzing, through morphoanatomical data and histochemical and phytochemical studies of essential oil, the samples present structural differences and differences in their chemical composition. Anatomical and histochemical analyses were performed by transverse, using longitudinal sections of 8 μm to 10 μm to perform epidermal dissociation, diaphonization, and tests to identify classes of secondary metabolites. The essential oils were isolated by hydrodistillation, and the identification of the chemical composition was performed by gas chromatography coupled with mass spectrometry. The anatomical study shows that there is no difference between specimens collected in different locations, and stellate trichomes were identified. The histochemical study detected total lipids and acids, terpenes, polysaccharides, phenolic compounds, tannins, alkaloids, and calcium oxalate. The low essential oil yield may be related to the low density of secretory cells (glandular trichomes), the unidentified compounds in the highest concentration in the essential oil were in relation to the chemical composition of the essential oils, and the major compounds were α-pinene, sabinene, (E)-caryophyllene, caryophyllene oxide, and oct-1-en-3-ol. The results provide new information about the anatomy and histochemistry of P. ornatus.

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Section: Resultsmentioning

confidence: 99%

Morphoanatomical, Histochemical, and Essential Oil Composition of the Plectranthus ornatus Codd. (Lamiaceae)

da Silva,

Correia,

Gurgel

et al. 2023

Molecules

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“…Three genetic variants, which Cantacessi et al (2008) called operational taxonomic units (OTUs) x, y, and z were proposed as novel species of domestic chickens: Eimeria lata, Eimeria nagambie, and Eimeria zaria, respectively (Blake et al, 2021). The nomenclature proposed by Blake et al ( 2021) is adopted throughout this manuscript, especially when studying cryptic species (Allgayer et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

Soares Júnior,

Itoyama,

Beretta

et al. 2023

Rev. Bras. Parasitol. Vet.

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The objective of this study was to identify Eimeria spp. in alternative poultry production systems (APPS) in the State of São Paulo, Brazil. Fecal samples (168) and DNA extracted from fecal samples obtained in APPS located in different Municipalities in the State of São Paulo (93) were examined by microscopy or genera-specific PCR (ITS-1 locus). Samples positive for Eimeria spp. were examined using Eimeria lata, Eimeria nagambie, and Eimeria zaria species-specific PCR protocols (ITS-2 locus) and another E. lata-specific PCR (candidate IMP1 genomic locus) followed by molecular cloning (E. lata and E. zaria ITS-2 amplicons) and genetic sequencing. All positive DNA samples were also submitted to genera-specific nested PCR (18S rRNA gene) followed by next-generation sequencing to identify Eimeria spp. Eimeria nagambie, E. zaria, and Eimeria sp. were identified by ITS2-targeted species-specific PCRs and genetic sequencing. Next-generation sequencing identified, in order of prevalence: E. nagambie; Eimeria acervulina; Eimeria mivati; Eimeria praecox; Eimeria brunetti; Eimeria mitis; Eimeria sp.; Eimeria maxima; E. zaria, and Eimeria necatrix/tenella. Our results confirmed, for the first time in Brazil, the identification of E. nagambie, E. zaria, and Eimeria spp. ITS-2 and 18S rRNA gene sequences not yet described in Brazil.

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Uma Análise Epistêmica Para a Elucidação Do Complexo De Espécies Crípticas

Abstract: do Vale do Rio dos Sinos (2015), com ênfase em Filosofia da Biologia. Doutorado em Biologia (2019) pela mesma instituição na área de Epistemologia e Mastozoologia de Roedores.

Cited by 2 publications

References 12 publications

Morphoanatomical, Histochemical, and Essential Oil Composition of the Plectranthus ornatus Codd. (Lamiaceae)

Morphoanatomical, Histochemical, and Essential Oil Composition of the Plectranthus ornatus Codd. (Lamiaceae)

Identification of Eimeria spp. in domestic chickens raised in alternative poultry production systems in the State of São Paulo, Brazil

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