Umbelliferone Increases the Expression of Adipocyte‐Specific Genes in 3 T3‐L1 Adipocyte

Abstract

Umbelliferone (UMB), a natural product of coumarin family, has been shown to reduce blood glucose and to improve lipid profiles in streptozotocin (STZ)-induced diabetic rats. Our objective was to examine the effect of UMB on adipogenesis by investigating its stimulatory effect on lipid accumulation and mRNA expression of adipogenic transcription factors and adipocyte-specific genes in 3 T3-L1 preadipocyte culture. An Oil Red O staining was used to monitor lipid accumulation, and we found that UMB treatment at concentration range of 10-100 μM significantly increased lipid accumulation of differentiating 3 T3-L1 cells. At the molecular level of adipogenesis, we examined the mRNA expression of adipogenic transcription factors, peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c. Those transcription factors were increased by UMB at 10-100 μM. Interestingly, UMB also stimulated the mRNA expression of adipocyte-specific genes, adipocyte fatty acid-binding protein, lipoprotein lipase, fatty acid synthase, fatty acid translocase, and adiponectin. Our findings indicate that the stimulatory effect of UMB on adipocyte differentiation likely occurs through up-regulation of adipogenic transcription factors and downstream adipocyte-specific gene expression.

Umbelliferone ameliorates cerebral ischemia–reperfusion injury via upregulating the PPAR gamma expression and suppressing TXNIP/NLRP3 inflammasome

“…UMB has been reported to exhibit neuroprotective effects in MPTP-induced mouse model and cross the blood-brain barrier, indicating that UMB has the potential to protect neuron from apoptosis [9]. UMB positively regulates peroxisome proliferator-activated receptor-␥ (PPAR-␥) activity and this action is involved in amelioration of metabolic disorders and alcohol-induced fatty liver [10][11][12]. PPAR-␥, belonging to the nuclear hormone receptor superfamily, has been certified as an important regulator of apoptosis, oxidative stress and inflammation [13][14][15], and also plays a significant role in the neuroprotection against cerebral ischemia-reperfusion injury [16].…”

Umbelliferone Improves an Impaired Glucose and Lipid Metabolism in High-Fat Diet/Streptozotocin-Induced Type 2 Diabetic Rats

“…Umbelliferone can act as an antihyperglycemic agent in STZ‐induced type 1 diabetic rats (Ramesh and Pugalendi, ). It has been reported that UMB can stimulate adipogenesis in 3 T3‐L1 cells (Naowaboot et al, ). A recent study found that UMB and 4‐methylumbelliferone reduced the hypertriglyceridemia and hyperglycemia in HFD‐induced obese mice (Sim et al, ).…”