Umbilical cord blood banks in the UK

Proctor, S J; Dickinson, Anne M.; Parekh, T; Chapman, Claire

doi:10.1136/bmj.323.7304.60

Cited by 7 publications

(4 citation statements)

References 9 publications

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“…2004). In developed areas of the world, including Europe and North America, the number of cord blood banks is increasing, providing the prospect of being able to select an ADS unit with an appropriate immunological phenotype for individual patients (Proctor et al . 2001; Warwick & Armitage 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Ontogenically, because of the relatively untapped length of these cells' telomeres, successful UCB-based haematotherapy protocols have already been explored (Dickinson et al 2002;Rocha et al 2004). In developed areas of the world, including Europe and North America, the number of cord blood banks is increasing, providing the prospect of being able to select an ADS unit with an appropriate immunological phenotype for individual patients (Proctor et al 2001;Warwick & Armitage 2004). These unique primitive cord blood-derived embryoniclike stem cells) offer multiple advantages over truly adult stem cells and over embryonic stem cells, and are a viable and ethically sound alternative to them for tissue bio-engineering applications.…”

Section: Figure 3 Cord Blood-derived Embryonic-like Stem Cells' Prodmentioning

confidence: 99%

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Production of stem cells with embryonic characteristics from human umbilical cord blood

McGuckin

Forraz²,

Baradez³

et al. 2005

Cell Proliferation

194

120

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When will embryonic stem cells reach the clinic? The answer is simple -- not soon! To produce large quantities of homogeneous tissue for transplantation, without feeder layers, and with the appropriate recipient's immunological phenotype, is a significant scientific hindrance, although adult stem (ADS) cells provide an alternative, more ethically acceptable, source. The annual global 100 million human birth rate proposes umbilical cord blood (UCB) as the largest untouched stem cell source, with advantages of naive immune status and relatively unshortened telomere length. Here, we report the world's first reproducible production of cells expressing embryonic stem cell markers, - cord-blood-derived embryonic-like stem cells (CBEs). UCB, after elective birth by Caesarean section, has been separated by sequential immunomagnetic removal of nucleate granulocytes, erythrocytes and haemopoietic myeloid/lymphoid progenitors. After 7 days of high density culture in microflasks, (10(5) cells/ml, IMDM, FCS 10%, thrombopoietin 10 ng/ml, flt3-ligand 50 ng/ml, c-kit ligand 20 ng/ml). CBE colonies formed adherent to the substrata; these were maintained for 6 weeks, then were subcultured and continued for a minimum 13 weeks. CBEs were positive for TRA-1-60, TRA-1-81, SSEA-4, SSEA-3 and Oct-4, but not SSEA-1, indicative of restriction in the human stem cell compartment. The CBEs were also microgravity--bioreactor cultured with hepatocyte growth medium (IMDM, FCS 10%, HGF 20 ng/ml, bFGF 10 ng/ml, EGF 10 ng/ml, c-kit ligand 10 ng/ml). After 4 weeks the cells were found to express characteristic hepatic markers, cytokeratin-18, alpha-foetoprotein and albumin. Thus, such CBEs are a viable human alternative from embryonic stem cells for stem cell research, without ethical constraint and with potential for clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Figure 3 Cord Blood-derived Embryonic-like Stem Cells' Prodmentioning

confidence: 99%

Production of stem cells with embryonic characteristics from human umbilical cord blood

McGuckin

Forraz²,

Baradez³

et al. 2005

Cell Proliferation

194

120

View full text Add to dashboard Cite

show abstract

“…Cord blood is a rich source of cytokines and growth factors which are poorly present in adult blood [22]. We transfused cord blood in various degenerative diseases such as aplastic anemia, vitiligo, retinitis pigmentosa, and other geriatric diseases and the results were encouraging.…”

Section: Discussionmentioning

confidence: 88%

A Study of 120 Umbilical Cord Whole Blood Transfusions in 77 Patients with Different Clinical Conditions

Sharma¹,

Agrawal²,

Bindal³

et al. 2017

IOSR JDMS

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“…The major disadvantage is low stem cell dose for larger patients. 7 Cure of CGD can be achieved by stem cell transplantation, and the best results are achieved when this is performed early in the course of the disease before serious infections and inflammatory sequelae have developed. We report a patient with X-linked CGD, who underwent a successful HLA-identical sibling umbilical cord stem cell transplant (UCSCT) after myeloablative conditioning.…”

mentioning

confidence: 99%

Successful umbilical cord blood stem cell transplantation for chronic granulomatous disease

Bhattacharya

Slatter

Curtis

et al. 2003

Bone Marrow Transplant

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Summary:Chronic granulomatous disease (CGD) causes growth failure, inflammatory lung damage and often early death. Prophylactic cotrimoxazole improves medium-term survival, but cannot prevent inflammatory sequelae. We report the first patient with CGD who underwent successful HLA identical sibling umbilical cord stem cell transplantation (UCSCT) after myeloablative conditioning. The patient presented with colitis, confirmed as CGD at 2 years of age. Following BU16/CY200 conditioning, he had UCSCT from his unaffected HLA identical sister. A year post-transplant, his colitis had resolved clinically and on radioisotope scan growth has improved. Neutrophil oxidative burst was 92% normal with full donor lymphocyte reconstitution.

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Umbilical cord blood banks in the UK

Cited by 7 publications

References 9 publications

Production of stem cells with embryonic characteristics from human umbilical cord blood

Production of stem cells with embryonic characteristics from human umbilical cord blood

A Study of 120 Umbilical Cord Whole Blood Transfusions in 77 Patients with Different Clinical Conditions

Successful umbilical cord blood stem cell transplantation for chronic granulomatous disease

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