Umbilical Cord Blood for Autologous Transfusion in the Early Postnatal Ontogeny: Analysis of Cell Composition and Viability during Long-Term Culturing

Abstract

Changes in cell composition and viability as well as the content and functional activity of hemopoietic progenitor cells were analyzed during long-term (up to 1 month at 4°C) storage of human umbilical cord blood cells. No significant quantitative changes in erythrocytes were found during this period. The total content and viability of leukocytes changed, which resulted in the prevalence of mononuclear cells (lymphocytes and monocytes). Analysis of functional activity of hemopoietic stem cells in semisolid culture revealed a decrease in the relative content of CFU during the first week of storage [corrected] and inability of cells to colony formation after 2 weeks.

Placenta and Placental Derivatives in Regenerative Therapies: Experimental Studies, History, and Prospects

“…Besides the transplantation of hematopoietic cord blood cells, a special interest of the researchers is attracted by erythrocytes and platelets of the cord blood [ 43 – 45 ]. The main distinction of cord blood erythrocytes from the erythrocytes of an adult is the content of fetal hemoglobin.…”