Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells

Zhou, Ting; Sun, Yan; Wang, Yanli; Chen, Xiaobing; Luo, Zhuo; Bu, Lin; Suo, Xu; Han, Jiayan; Li, Xiaomin; Shi, Jun

doi:10.1007/s10753-019-00960-z

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…Endothelial cells line the blood vessels of various organs and undergo pathophysiological changes during the course of sepsis [ 24 , 25 ]. PMA-activated THP-1 cells can replace human macrophages in in vitro tests [ 26 ]. The in vitro models of sepsis were successfully established via stimulating HUVEC and macrophages by LPS for 24 hours, which showed pyroptosis of HUVECs and macrophages with the release of proinflammatory factors IL-1 β and IL-18.…”

Section: Discussionmentioning

confidence: 99%

Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response

Luo

Chen

Sun

et al. 2020

BioMed Research International

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Pyroptosis, an inflammatory form of programmed cell death, is the initiating event of sepsis and results in immune imbalance by releasing IL-1β and IL-18 in the early stages. Studies show that enhancing autophagy via genetic manipulation can inhibit pyroptosis and prolong the survival of a sepsis animal model, indicating a possible therapeutic strategy against sepsis. However, almost no study so far has achieved pyroptosis inhibition via pharmacological autophagy induction in a sepsis disease model. To this end, we established an in vitro sepsis model by stimulating primary human umbilical vein endothelial cells (HUVECs) with lipopolysaccharide (LPS), and analyzed the effect of the autophagy agonist rapamycin (RAPA) on pyroptosis. Phorbol 12-myristate 13-acetate- (PMA-) activated human THP-1 cells were used as the positive control. LPS significantly increased the levels of the pyroptotic protein Gasdermin D (GSDMD), cysteinyl aspartate-specific proteinase 1 (caspase-1), secreted LDH, IL-1β, and IL-18. RAPA treatment downregulated the above factors and enhanced autophagy in the LPS-stimulated HUVECs and THP-1 cells. This study shows that RAPA abrogates LPS-mediated increase in IL-1β and IL-18 by inhibiting pyroptosis and enhancing autophagy.

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Section: Discussionmentioning

confidence: 99%

Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response

Luo

Chen

Sun

et al. 2020

BioMed Research International

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“…MSCs could release anti-inflammatory (such as interleukin-10 [IL-10], IL-13), pro-inflammatory (such as IL-8, IL-1α, IL-12), and pleiotropic cytokines (IL-6, IL-11, IL-16, IL-1β) to regulate immune function after ischemic stroke. In macrophages in vitro, UCMSCs increased the lipopolysaccharide-stimulated expression levels of IL-10 and IL-37 through PI3K/Akt signaling pathway to play an anti-inflammatory effect [ 24 ]. Further, transplantation of genetically engineered MSCs that overexpress IL‐10 (MSCs‐IL‐10) significantly increased autophagy, mitophagy, and cell survival markers, along with decreased markers for cell death and neuroinflammation than MSCs alone in vivo [ 25 ].…”

Section: Potential Mechanisms and Therapeutic Targets Of Msc Transpla...mentioning

confidence: 99%

Potential mechanisms and therapeutic targets of mesenchymal stem cell transplantation for ischemic stroke

et al. 2022

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Ischemic stroke is one of the major causes of death and disability in the world. Currently, most patients cannot choose intravenous thrombolysis or intravascular mechanical thrombectomy because of narrow therapeutic windows and severe complications. Stem cell transplantation is an emerging treatment and has been studied in various central nervous system diseases. Animal and clinical studies showed that transplantation of mesenchymal stem cells (MSCs) could alleviate neurological deficits and bring hope for ischemic stroke treatment. This article reviewed biological characteristics, safety, feasibility and efficacy of MSCs therapy, potential therapeutic targets of MSCs, and production process of Good Manufacturing Practices-grade MSCs, to explore the potential therapeutic targets of MSCs in the process of production and use and provide new therapeutic directions for ischemic stroke.

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“…and 50% RPMI-1640 culture medium [40]. The cell concentration was adjusted to 10 6 cells/mL, followed by inoculation in 60-mm Petri dishes.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…When the number of cells was sufficient, a part of the cells was retrieved and frozen. Cryopreservation solution consisted of 10 % DMSO, 40 % fetal bovine serum, and 50 % RPMI-1640 culture medium [40]. The cell concentration was adjusted to 10 6 cells/mL, followed by inoculation in 60-mm Petri dishes.…”

Section: Cell Culture and Drug Administrationmentioning

confidence: 99%

Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-κB/NLRP3 Pathway to Alleviate LPS-induced Acute Lung Injury

Zhang

Zhou

et al. 2022

Planta Med

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Acute lung injury (ALI) is one of the representative “lung heat syndromes” in traditional Chinese medicine (TCM). Scutellaria baicalensis is a herbal medicine used in TCM for treating lung diseases due to its remarkable anti-inflammatory and antiviral effects. When used in TCM, S. baicalensis root is divided into two categories: S. baicalensis pith-not-decayed root (SN) and S. baicalensis pith-decayed root (SD). Compared to SN, SD has a better effect on lung diseases. We constructed a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model to study the pharmacodynamic mechanism of SD. The ethanolic extract of Scutellaria baicalensis pith-decayed root (EESD) significantly affected LPS-induced ALI by reducing alveolar interstitial thickening, pulmonary edema, and other pathological symptoms, decreasing the infiltration of inflammatory cells, especially macrophages, and inhibiting IL-1β, TNF-α, and IL-6 transcription and translation. Furthermore, in the THP-1 macrophage model induced by LPS, EESD inhibited the expression of phosphorylated nuclear factor inhibitory protein alpha (p-IκBα), phosphorylated nuclear factor-κB P65 (p-p65), cleaved-caspase-1, cleaved-IL-1β protein, and the release of inflammatory factors in the NF-κB/NLRP3 pathway, inhibiting macrophage function. In vivo experiments yielded similar results. Therefore, the present study clarified the potential of EESD in the treatment of ALI and revealed its potential pharmacodynamic mechanism by inhibiting the NF-κB/NLRP3 inflammasome pathway and suppressing the pro-inflammatory phenotype activation of lung tissue macrophages.

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Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells

Cited by 8 publications

References 47 publications

Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response

Rapamycin Inhibited Pyroptosis and Reduced the Release of IL-1β and IL-18 in the Septic Response

Potential mechanisms and therapeutic targets of mesenchymal stem cell transplantation for ischemic stroke

Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-κB/NLRP3 Pathway to Alleviate LPS-induced Acute Lung Injury

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