Un‐filtered recordings from insect taste sensilla

Marion‐Poll, Frédéric; Pers, Jan van der

doi:10.1111/j.1570-7458.1996.tb00899.x

Cited by 62 publications

(33 citation statements)

References 8 publications

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“…Although methanol is thought to damage the nerve cells, which then does not allow for long recording durations, and most likely influences impulse frequencies, it has the advantage of dissolving material obstructing the pores and helping to obtain a good contact (Maher and Thiery 2004). Electrophysiological responses from the mesothoracic distal tarsomere were recorded by combining different techniques previously used to study single chemosensory sensilla (Hodgson et al 1955;den Otter and van der Starre 1967;Marion-Poll and van der Pers 1996;Solinas et al 2001;Maher and Thiery 2004;Ganassi et al 2007). The indifferent electrode, a glass micropipette (tip 4-5μm diameter) filled with a ringer solution (Kaissling 1995), was inserted into the aphid prothorax.…”

Section: Methodsmentioning

confidence: 99%

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

et al. 2009

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We report the effects of some bisorbicillinoids isolated from biomass of the fungus Trichoderma citrinoviride on settling and feeding preference of the aphid Schizaphis graminum. Purification of the fungal metabolites was carried out by a combination of column chromatography and thin-layer chromatography using direct and reverse phases. Chemical identification was performed by spectroscopic methods including nuclear magnetic resonance and mass spectrometry. The identified bisorbicillinoids appeared to be bislongiquinolide, its 16,17-dihydro derivative, trichodimerol, and dihydrotrichodimerol. A feeding preference test with alate morphs of S. graminum was used to identify the active fractions. Among the four bisorbicillinoids, dihydrotrichodimerol and bislongiquinolide influenced aphid feeding preference, restraining specimens from settling on leaves treated with metabolites. Taste neurons sensitive to these compounds, particularly to bislongiquinolide, were located on tarsi of the S. graminum alate morphs.

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Section: Methodsmentioning

confidence: 99%

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

et al. 2009

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“…Contacting a taste sensillum with the stimulus electrode triggered a data acquisition sequence (sampling rate, 10 kHz, 12 bits; DT2821 Data Translation), which was stored as an individual file on a computer. Data were then analyzed with AWAVE software (36). Spikes were detected and analyzed by using interactive procedures from a custom-made software package, DBWAVE. To express DTI (31-33) in a limited number of GAL4-expressing neurons in the labellum of NP1017, we used the mosaic analysis with a repressible cell marker system (34).…”

Section: Methodsmentioning

confidence: 99%

Cellular identification of water gustatory receptor neurons and their central projection pattern in Drosophila

Inoshita

Tanimura²

2006

Proc. Natl. Acad. Sci. U.S.A.

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Water perception is important for insects, because they are particularly vulnerable to water loss because their body size is small. In Drosophila, gustatory receptor neurons are located at the base of the taste sensilla on the labellum, tarsi, and wing margins. One of the gustatory receptor neurons in typical sensilla is known to respond to water. To reveal the neural mechanisms of water perception in Drosophila, it is necessary to identify water receptor neurons and their projection patterns. We used a Gal4 enhancer trap strain in which GAL4 is expressed in a single gustatory receptor neuron in each sensillum on the labellum. We investigated the function of these neurons by expressing the upstream activating sequence transgenes, shibire ts1 , tetanus toxin light chain, or diphtheria toxin A chain. Results from the proboscis extension reflex test and electrophysiological recordings indicated that the GAL4-expressing neurons respond to water. We show here that the water receptor neurons project to a specific region in the subesophageal ganglion, thus revealing the water taste sensory map in Drosophila.taste ͉ water receptor neuron C hemical senses are necessary for all animals to perceive environmental chemical information. In insects, contact chemoreception plays a role in a variety of behaviors, including feeding, courtship, and oviposition site choice. Because sophisticated genetic tools can be used in Drosophila, this organism offers an excellent system to explore the neural and molecular mechanisms of taste (1). The chemosensory organs of Drosophila are composed of bristles called sensilla that are located on the labellum, tarsi, wing margins, and ovipositor. There are usually four gustatory receptor neurons and one mechanosensory neuron at the base of a typical sensillum (2, 3). Electrophysiological studies illustrate that these bipolar receptor neurons respond to different stimuli such as sugar, salt, water, and deterrent compounds (4-6). Although perception of water induces feeding behavior of flies when they are thirsty, there is less information about neural mechanisms of water perception. The axons of gustatory receptor neurons in the labellum project to the subesophageal ganglion (SOG), and those of the tarsi project to the thoracic-abdominal ganglion or to the SOG. Previous studies using horseradish peroxidase (HRP) as a marker reported projection patterns of gustatory receptor neurons on the labellum (7-10) and described seven types of projection patterns. Recently, the Gr family of putative gustatory receptor genes was identified. Understanding the function of this protein group may clarify the neural mechanism underlying the sensory coding of taste (11-13). Grs encode G protein-coupled receptors with seven transmembrane domains, such as the sweet and bitter taste receptors found in vertebrates (14-16). One of these Grs, Gr5a, is required for the sensory response of Drosophila to trehalose (17)(18)(19). The gene expression pattern of Gr5a was observed by using a Gr5a promoter-Gal4 strain (19-21). R...

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“…When a taste neuron is excited to generate impulses, fluctuations in potential between the two electrodes are recorded and amplified through a high-input impedance preamplifier circuit. An important feature of the preamplifier circuit that was developed later is that it compensates for the large offset potential between the sensillum and the reference electrode (typically 100 mV) (Marion-Poll and van der Pers 1996). The signal is then filtered (e.g., low pass 3 kHz, high pass 0.1 kHz), further amplified (100×-1000×), and transferred to a computer (sampling rate typically ≥10 kHz) that has the appropriate software for storing and analyzing the data.…”

Section: Electrophysiological Recording From the Gustatory Systemmentioning

confidence: 99%

Electrophysiological Recording from Drosophila Taste Sensilla

Benton¹,

Dahanukar²

2011

Cold Spring Harb Protoc

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INTRODUCTIONThe chemical senses smell and taste, detect and discriminate an enormous diversity of environmental stimuli and provide fascinating but challenging models to investigate how sensory cues are represented in the brain. Important stimulus-coding events occur in peripheral sensory neurons, which express specific combinations of chemosensory receptors with defined ligand-response profiles. These receptors convert ligand recognition into spatial and temporal patterns of neural activity that are transmitted to and interpreted in central brain regions. Drosophila provides an attractive model to study chemosensory coding, because it possesses relatively simple peripheral olfactory and gustatory systems that display many organizational parallels to those of vertebrates. Moreover, virtually all of the peripheral chemosensory neurons are easily accessible for physiological analysis, as they are exposed on the surface of sensory organs in specialized sensory hairs called sensilla. In recent years, improvements in microscopy and instrumentation for electrode manipulation have opened up the much smaller Drosophila system to electrophysiological techniques, powerfully complementing many years of molecular genetic studies. As with most electrophysiological methods, there is probably no substitute for learning this technique directly from a laboratory in which it is already established. This protocol describes the basics of setting up the electrophysiology rig and stimulus delivery device, sample preparation, and performing and analyzing recordings of stimulus-evoked activity from Drosophila taste sensilla.

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Un‐filtered recordings from insect taste sensilla

Cited by 62 publications

References 8 publications

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

Cellular identification of water gustatory receptor neurons and their central projection pattern in Drosophila

Electrophysiological Recording from Drosophila Taste Sensilla

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