Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis

Чернов, В. М.; Чернова, О. А.; Medvedeva, E. S.; Mouzykantov, Alexey A.; Пономарева, А. А.; Shaymardanova, G. F.; Gorshkov, Oleg; Trushin, Maxim V.

doi:10.1016/j.jprot.2011.07.016

Cited by 17 publications

(21 citation statements)

References 26 publications

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“…Acholeplasma is a widely distributed mycoplasma in nature and possesses very versatile adaptation capabilities [Chernov et al, 2005]. Among all its ecological capabilities, it is a causative agent of some plant diseases [Chernov et al, 2007[Chernov et al, , 2011. The successful virulence traits (infectivity, invasivity, toxigenicity and persistence) of Acholeplasma towards plants suggest that it can survive unfavorable conditions, such as oxidative stress, limited substrate and temperature fluctuations [Chernov et al, 2011].…”

Section: Potentially Phytopathogens In the Wormbed Leachatementioning

confidence: 99%

“…Among all its ecological capabilities, it is a causative agent of some plant diseases [Chernov et al, 2007[Chernov et al, , 2011. The successful virulence traits (infectivity, invasivity, toxigenicity and persistence) of Acholeplasma towards plants suggest that it can survive unfavorable conditions, such as oxidative stress, limited substrate and temperature fluctuations [Chernov et al, 2011]. It has been proposed that phytotoxicity is one of the most important criteria for evaluating the suitability of leachate for plant applications Carballo et al, 2009;Tang et al, 2006;Xu et al, 2012].…”

Section: Potentially Phytopathogens In the Wormbed Leachatementioning

confidence: 99%

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Changes in the Bacterial Community Structure in Stored Wormbed Leachate

et al. 2014

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Organic wastes, such as cow manure, are often composted with earthworms (vermicomposting) while excess water is drained and collected. This wormbed leachate is nutrient-rich and it has been extensively used to fertilize plants. However, it is derived partially from a not yet finished compost process and could exhibit phytotoxicity or contain potentially hazardous microorganisms. The bacterial community in wormbed leachate derived from vermicomposting of cow manure was studied by pyrosequencing the 16S rRNA gene. The fresh wormbed leachate was rich in Mollicutes, particularly the genus Acholeplasma which contain phytopathogen species. The abundance of the Mollicutes decreased when the leachate was stored, while that of the Rhizobiales and the genus Pseudomonas increased. The bacterial communities changed rapidly in the leachate during storage. The changes in ammonium, nitrate and inorganic carbon content of the wormbed leachate when stored were correlated to changes in the bacterial community structure. It was found that storage of the wormbed leachate might be required before it can be applied to crops as large proportions of potentially plant pathogens were found in the fresh leachate.

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Section: Potentially Phytopathogens In the Wormbed Leachatementioning

confidence: 99%

Section: Potentially Phytopathogens In the Wormbed Leachatementioning

confidence: 99%

Changes in the Bacterial Community Structure in Stored Wormbed Leachate

et al. 2014

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“…The development of resistance to quinolones is associated with mutations in genes that encode antibiotic-targeted proteins, and a limitation is that the antibiotics used to treat microbial cells are not considered to be effective against mycoplasmas [4, 8]. Thus, it would be beneficial to elucidate the mechanisms that facilitate the rapid development of antibiotic resistance to allow the treatment of mycoplasma infections, which appear to be associated with the adaptation of mycoplasmas to stress conditions [9, 10]. The successful implementation of genome projects for a number of mycoplasmas has opened up the possibility of using postgenomic technologies to study their antibiotic resistance processes [11, 12].…”

Section: Introductionmentioning

confidence: 99%

“…In our study [9, 10], transcriptome-proteome analysis and nanoscopy were identified as the stress-reactive proteins and genes of Acholeplasma laidlawii , which showed that the adaptation of this mycoplasma to stressful factors was related to the production of extracellular vesicles (EVs). The EVs of bacteria are spherical nanostructures surrounded by a membrane (20–200 nm in diameter), which mediate the traffic of a wide variety of compounds that participate in signaling, intercellular interactions, and pathogenesis [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of Mycoplasmas to Antimicrobial Agents:Acholeplasma laidlawiiExtracellular Vesicles Mediate the Export of Ciprofloxacin and a Mutant Gene Related to the Antibiotic Target

Medvedeva

Baranova

Mouzykantov

et al. 2014

The Scientific World Journal

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This study demonstrated that extracellular membrane vesicles are involved with the development of resistance to fluoroquinolones by mycoplasmas (class Mollicutes). This study assessed the differences in susceptibility to ciprofloxacin among strains of Acholeplasma laidlawii PG8. The mechanisms of mycoplasma resistance to antibiotics may be associated with a mutation in a gene related to the target of quinolones, which could modulate the vesiculation level. A. laidlawii extracellular vesicles mediated the export of the nucleotide sequences of the antibiotic target gene as well as the traffic of ciprofloxacin. These results may facilitate the development of effective approaches to control mycoplasma infections, as well as the contamination of cell cultures and vaccine preparations.

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“…Control of mycoplasma infections presents a serious problem whose resolution is connected with clarification of mechanisms of mycoplasma adaptation to the environment, formation of the “parasite-host” system, and realization of virulence. [2, 4]. Sequencing of A. laidlawii genome [5] provided an opportunity for application of postgenomic technologies for detection of molecular-genetic fundamentals of mycoplasma survival in different environments [2].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Membrane Vesicles and Phytopathogenicity ofAcholeplasma laidlawiiPG8

Чернов

Чернова

Mouzykantov

et al. 2012

The Scientific World Journal

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For the first time, the phytopathogenicity of extracellular vesicles of Acholeplasma laidlawii PG8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in Oryza sativa L. plants was studied. Data on the ability of extracellular vesicles of Acholeplasma laidlawii PG8 to penetrate from the nutrient medium into overground parts of Oryza sativa L. through the root system and to cause alterations in ultrastructural organization of the plants were presented. As a result of the analysis of ultrathin leaf sections of plants grown in medium with A. laidlawii PG8 vesicles, we detected significant changes in tissue ultrastructure characteristic to oxidative stress in plants as well as their cultivation along with bacterial cells. The presence of nucleotide sequences of some mycoplasma genes within extracellular vesicles of Acholeplasma laidlawii PG8 allowed a possibility to use PCR (with the following sequencing) to perform differential detection of cells and bacterial vesicles in samples under study. The obtained data may suggest the ability of extracellular vesicles of the mycoplasma to display in plants the features of infection from the viewpoint of virulence criteria—invasivity, infectivity—and toxigenicity—and to favor to bacterial phytopathogenicity.

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Unadapted and adapted to starvation Acholeplasma laidlawii cells induce different responses of Oryza sativa, as determined by proteome analysis

Cited by 17 publications

References 26 publications

Changes in the Bacterial Community Structure in Stored Wormbed Leachate

Changes in the Bacterial Community Structure in Stored Wormbed Leachate

Adaptation of Mycoplasmas to Antimicrobial Agents:Acholeplasma laidlawiiExtracellular Vesicles Mediate the Export of Ciprofloxacin and a Mutant Gene Related to the Antibiotic Target

Extracellular Membrane Vesicles and Phytopathogenicity ofAcholeplasma laidlawiiPG8

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