Unanticipated stimulatory action of glucocorticoids on epithelial calcium absorption. Effect of dexamethasone on rat distal colon.

Lee, David B. N.

doi:10.1172/jci110772

Cited by 19 publications

(4 citation statements)

References 25 publications

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“…Although renal CaBP-9k levels were significantly decreased in adrenalectomized mice from which endogenous cortisol had been removed by a surgical method, we did not observe any difference in expression of intestinal CaBP-9k mRNA or protein, compared with sham-operated animals. It is widely accepted that glucocorticoids reduce intestinal calcium absorption by inhibition of active calcium transcellular transport and brush border vehicle uptake [ 29 , 30 ]. In addition, cortisol reduced the production of intestinal calcium binding protein in chicks [ 31 ].…”

Section: Other Physiological Factors That Affect Cabp-9k Expressionmentioning

confidence: 99%

Biological Significance of Calbindin-D9k within Duodenal Epithelium

Hong

Jeung

2013

IJMS

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Calbindin-D9k (CaBP-9k) binds calcium with high affinity and regulates the distribution of free calcium in the cytoplasm. The expression of CaBP-9k is detected primarily in intestine that is vitamin D target tissue, and accumulates in the enterocytes of the duodenal villi. These enterocytes are the clearest example of vitamin D responsive cells, and the presence of CaBP-9k within them accentuates calcium absorption mediated by active transcellular calcium transport. It has been well established that the expression of CaBP-9k is mediated with vitamin D response element on its promoter and it regulates the amount of intracellular calcium in order to prevent cell death from reaching the toxicity of free calcium. There is now little doubt that glucocorticoid also decreases CaBP-9k expression in duodenal epithelial cells. In addition, it was reported that the level of CaBP-9k gene in enterocytes is increased in pregnancy when the plasma estradiol concentration is generally associated with a concomitant increase. Although calcium homeostasis was not disturbed in mice lacking the CaBP-9k gene, we found that CaBP-9k has a buffering role of free calcium in the cytosolic environment beyond that of calcium transfer. To expand our knowledge of the biological functions of CaBP-9k, our research has focused on defining the biological significance of intracellular CaBP-9k. Our findings suggest that the CaBP-9k gene is involved in compensatory induction of other calcium transporter genes in duodenal epithelial cells. This article summarizes the findings from recent studies on the expression and the functions of CaBP-9k in the small intestine.

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Section: Other Physiological Factors That Affect Cabp-9k Expressionmentioning

confidence: 99%

Biological Significance of Calbindin-D9k within Duodenal Epithelium

Hong

Jeung

2013

IJMS

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“…At the end of each experiment, theophylline was added to the buffer solutions bathing both mucosal and serosal sides of jejunal segments. An increase in both I,, and PD was an indication of viable tissue (Lee, 1983). Like I,,, PD was also depressed when PG was added to the tissue-bathing medium.…”

Section: Electrical Variables Of the Jejunal Segmentsmentioning

confidence: 90%

“…I,, was measured by a modification of the method of Ussing & Zerahn (1951), as previously described (Lee, 1983). The spontaneous potential difference (PD) across the tissue was abolished by passing a I,, after correction for fluid resistance using an automatic voltage clamp (Model AVC-3, Netronics, Inc., Hudson, MA).…”

Section: A T E R I a L S A N D Methodsmentioning

confidence: 99%

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Influence of prostaglandins on unidirectional zinc fluxes across the small intestine of the rat

1988

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I. The regulatory role of prostaglandins (PGs) E, and F,, on the zinc transport rate across the jejunal segments of rats was examined by employing the Ussing chamber technique. The Zn flux rate from mucosa to serosa across jejunal segments (JmJ was 5.24 (SE 1.54) nmol/h per cmz (n 48) and that from serosa to mucosa (Jsm) was 15.16 (SE 2.38) nmol/h per cma (n 48) when both sides of the segment were bathed with Ringer's bicarbonate solution containing 0.5 mM-zinc chloride and 3 mM-L-histidine.2. When 5.0 or 5 0 p~ of either PGE, or PGF,, were added to the serosal side of the tissue, J,, generally decreased and J,, generally increased, compared with controls. On the other hand, when PGE, or PGF,, was added to the mucosal side of the tissue, J,, either did not change or increased while J,, had a tendency to decrease.3. The Zn uptake capacity of tissue increased significantly when PG was added to the serosal side of the tissuebathing medium, but not when PG was added to the mucosal side. The uptake capacity of mucosal Zn by jejunal segments was approximately twice that of serosal Zn.4. When PG was included in the tissue-bathing medium. the short-circuit current, potential difference and conductance between the mucosa-and serosa-bathing media generally decreased.5. These results suggest that (a) PGs influence Zn flux rate not by chelating Zn and carrying it across the mucosal cell membrane but by interacting with the cytosolic components, (b) it is the serosal PGs which control the Zn flux rate and (c) PGs play a part in triggering a transduction mechanism in the intestinal Zn transport process.

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Metabolic bone disease in adults with inflammatory bowel disease

Adachi

Rostom

2007

Inflamm Bowel Dis

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Bone loss is seen frequently in those with intlammatory bowel disease (IBD). It appears to be more common in those with Crohn's disease than in those with ulcerative colitis. Corticosteroids play an important role in the development of osteoporosis. The prevalence of osteoporosis in IBD, the ef-fects of corticosteroids on bone metabolism, and treatment options are reviewed. Key Words: Inflammatory bowel disease-Crohn's disease-Ulcerative colitis-osteoporo- sis-Osteomalacia-Corticosteroid-induced osteoporosis.~ .~

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Unanticipated stimulatory action of glucocorticoids on epithelial calcium absorption. Effect of dexamethasone on rat distal colon.

Cited by 19 publications

References 25 publications

Biological Significance of Calbindin-D9k within Duodenal Epithelium

Biological Significance of Calbindin-D9k within Duodenal Epithelium

Influence of prostaglandins on unidirectional zinc fluxes across the small intestine of the rat

Metabolic bone disease in adults with inflammatory bowel disease

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