We evaluated the uncertainty relevant to s-process nucleosynthesis using a Monte-Carlo centred approach. We are based on a realistic and general prescription of temperature dependent uncertainty for the reactions. We considered massive stars for the weak s-process and AGB stars for the main s-process. We found that the adopted uncertainty for (n,γ) rates, tens of per cent on average, affect the production of s-process nuclei along the β-stability line, while for β-decay, for which contributions from excited states enhances the uncertainty, has the strongest impact on branching points.