Uncharted territories: Solving the mysteries of male meiosis in flies

Lee, LingSze; Rosin, Leah F.

doi:10.1371/journal.pgen.1011185

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…Another possibility is that homologs in Plodia remain linked at metaphase I by non-crossover mechanisms, such as those that have been reported in Drosophila males, Bombyx females, and C. elegans [48,[68][69][70][71][72][73][74]. The fact that we do not observe a strong correlation between chromosome length and center-oriented bivalents would, indeed, support a model where these center-oriented bivalents are not exclusively forming by double crossovers (which we would expect to be more frequent for longer chromosomes).…”

Section: Plos Geneticsmentioning

confidence: 99%

Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species

Hockens,

Lorenzi,

Wang

et al. 2024

PLoS Genet

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Precise regulation of chromosome dynamics in the germline is essential for reproductive success across species. Yet, the mechanisms underlying meiotic chromosomal events such as homolog pairing and chromosome segregation are not fully understood in many species. Here, we employ Oligopaint DNA FISH to investigate mechanisms of meiotic homolog pairing and chromosome segregation in the holocentric pantry moth, Plodia interpunctella, and compare our findings to new and previous studies in the silkworm moth, Bombyx mori, which diverged from P. interpunctella over 100 million years ago. We find that pairing in both Bombyx and Plodia spermatogenesis is initiated at gene-rich chromosome ends. Additionally, both species form rod shaped cruciform-like bivalents at metaphase I. However, unlike the telomere-oriented chromosome segregation mechanism observed in Bombyx, Plodia can orient bivalents in multiple different ways at metaphase I. Surprisingly, in both species we find that kinetochores consistently assemble at non-telomeric loci toward the center of chromosomes regardless of where chromosome centers are located in the bivalent. Additionally, sister kinetochores do not seem to be paired in these species. Instead, four distinct kinetochores are easily observed at metaphase I. Despite this, we find clear end-on microtubule attachments and not lateral microtubule attachments co-orienting these separated kinetochores. These findings challenge the classical view of segregation where paired, poleward-facing kinetochores are required for accurate homolog separation in meiosis I. Our studies here highlight the importance of exploring fundamental processes in non-model systems, as employing novel organisms can lead to the discovery of novel biology.

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Section: Plos Geneticsmentioning

confidence: 99%

Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species

Hockens,

Lorenzi,

Wang

et al. 2024

PLoS Genet

Self Cite

View full text Add to dashboard Cite

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Unusual modes of cell and nuclear divisions characterise Drosophila development

Yang,

Wijaya,

Kapoor

et al. 2024

Biochemical Society Transactions

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The growth and development of metazoan organisms is dependent upon a co-ordinated programme of cellular proliferation and differentiation, from the initial formation of the zygote through to maintenance of mature organs in adult organisms. Early studies of proliferation of ex vivo cultures and unicellular eukaryotes described a cyclic nature of cell division characterised by periods of DNA synthesis (S-phase) and segregation of newly synthesized chromosomes (M-phase) interspersed by seeming inactivity, the gap phases, G1 and G2. We now know that G1 and G2 play critical roles in regulating the cell cycle, including monitoring of favourable environmental conditions to facilitate cell division, and ensuring genomic integrity prior to DNA replication and nuclear division. M-phase is usually followed by the physical separation of nascent daughters, termed cytokinesis. These phases where G1 leads to S phase, followed by G2 prior to M phase and the subsequent cytokinesis to produce two daughters, both identical in genomic composition and cellular morphology are what might be termed an archetypal cell division. Studies of development of many different organs in different species have demonstrated that this stereotypical cell cycle is often subverted to produce specific developmental outcomes, and examples from over 100 years of analysis of the development of Drosophila melanogaster have uncovered many different modes of cell division within this one species.

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Uncharted territories: Solving the mysteries of male meiosis in flies

Cited by 2 publications

References 54 publications

Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species

Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species

Unusual modes of cell and nuclear divisions characterise Drosophila development

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