Unconventional Cell Division Cycles from Marine-Derived Yeasts

Mitchison-Field, Lorna M. Y.; Vargas-Muñiz, José M.; Stormo, Benjamin M; Vogt, Ellysa J.D.; Dierdonck, Sarah Van; Pelletier, James F.; Ehrlich, Christoph; Lew, Daniel J.; Field, Christine M.; Gladfelter, Amy S.

doi:10.1016/j.cub.2019.08.050

Cited by 50 publications

(69 citation statements)

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“…However, Mitchison‐Field et al . (2019) recently identified new division forms in marine isolates, such as spherical division, multiple budding or readily transitioning between both in a single generation, allowing for a single mother cell to generate several daughter cells at once. These findings highlight our currently limited knowledge on marine yeast ecology and the need for modern microscopy‐based techniques, such as the CARD‐FISH protocol presented here, to be incorporated into environmental surveys.…”

Section: Discussionmentioning

confidence: 99%

Diversity and biomass dynamics of unicellular marine fungi during a spring phytoplankton bloom

Priest

Fuchs

Amann

et al. 2020

Environmental Microbiology

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Section: Discussionmentioning

confidence: 99%

Diversity and biomass dynamics of unicellular marine fungi during a spring phytoplankton bloom

Priest

Fuchs

Amann

et al. 2020

Environmental Microbiology

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“…Most remarkably, yeast cells of Aureobasidium sp. generate variable numbers of buds simultaneously (Mitchison-Field et al, 2019), a capacity shared with the much more distantly related zygomycete Mucor circinelloides (Lee, Li, Calo, & Heitman, 2013). This phenotypic diversity may be enabled by a polarity circuit that allows a switch between competition, coexistence, and equalization behaviors in response to appropriate tuning of parameter values.…”

Section: Implications For Other Systemsmentioning

confidence: 99%

How cells determine the number of polarity sites

Chiou

Moran

Lew

2021

eLife

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The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells generate different numbers of polarity sites. Mass-conserved activator-substrate (MCAS) models that describe polarity circuits develop multiple initial polarity sites, but then those sites engage in competition, leaving a single winner. Theoretical analyses predicted that competition would slow dramatically as GTPase concentrations at different polarity sites increase toward a ‘saturation point’, allowing polarity sites to coexist. Here, we test this prediction using budding yeast cells, and confirm that increasing the amount of key polarity proteins results in multiple polarity sites and simultaneous budding. Further, we elucidate a novel design principle whereby cells can switch from competition to equalization among polarity sites. These findings provide insight into how cells with diverse morphologies may determine the number of polarity sites.

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“…Schizosaccharomyces pombe naturally switch from uni-polar to bi-polar growth during each cell cycle (Martin and Chang, 2005), Ashbya gossypii form branching hyphae that exhibit increasing number of polarity sites as each cell grows (Knechtle et al, 2003), and yeast cells of Aureobasidium sp. generate variable numbers of buds simultaneously (Mitchison-Field et al, 2019). This phenotypic diversity may be enabled by a polarity circuit that allows a switch between competition, coexistence, and equalization behaviors in response to appropriate tuning of parameter values.…”

Section: Implications For Other Systemsmentioning

confidence: 99%

How cells determine the number of polarity sites

Chiou

Moran

Lew

2020

Preprint

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The diversity of cell morphologies arises, in part, through regulation of cell polarity by Rho-family GTPases. A poorly understood but fundamental question concerns the regulatory mechanisms by which different cells can generate different numbers of polarity sites. Theoretical models of polarity circuits develop multiple initial polarity sites, but then those sites engage in competition, leaving a single winner. The timescale of competition slows dramatically as GTPase concentrations at polarity sites approach a "saturation point", allowing multiple sites to coexist. Here, we show that these principles hold in more complex mechanistic models of the Saccharomyces cerevisiae polarity machinery, and confirm model predictions in vivo. Further, we elucidate a novel design principle whereby cells can switch from competition to equalization among polarity sites. These findings provide insight into how cells determine the number of polarity sites.

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Unconventional Cell Division Cycles from Marine-Derived Yeasts

Abstract: Highlights d 35 fungal species were isolated from the ocean, sponge, coral, and coastal sediment d Time-lapse imaging reveals unusual cell cycles, cell-division patterns, and polarity

Cited by 50 publications

References 137 publications

Diversity and biomass dynamics of unicellular marine fungi during a spring phytoplankton bloom

Diversity and biomass dynamics of unicellular marine fungi during a spring phytoplankton bloom

How cells determine the number of polarity sites

How cells determine the number of polarity sites

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