Unconventional Extraction Methods of Oleaginous Yeast Cell Pretreatment and Disruption

Abstract

Extraction is one of the most commonly used methods for obtaining and purifying chemical compounds for commercial usage. The aim of this study was to evaluate the effect of unconventional permeabilization and cell disruption methods on the yield of lipid extraction from cells of the oleaginous yeast Yarrowia lipolytica. Batch cultures in a medium with molasses and waste post-frying oil were carried out. The biomass was subjected to pulsed electric field (PEF), high-pressure processing (HPP), ultrasounds (US), and several conventional processing techniques with chemical and mechanical agents (glass beads, acetone, Triton and Tween surfactants). The effectiveness of the applied methods, either on cell permeabilization or cell disruption, was investigated by analyzing the oil and total protein extraction yield and oil leaching efficiency, as well as by using microscope images. The PEF and US treatments proved to be effective permeabilization methods as a step of sample pretreatment for extraction. These unconventional physical methods could efficiently increase intracellular lipid extraction yield in solvent applications.

Nutritional and Medicinal Properties of Microbial Oil

“…The reality of using SCOs is far from being achieved, due to the high cost of their recovery from biomass. The costs can be lowered by the usage of a waste carbon source in the culture medium and by a reduction in the operational costs of SCO extraction, which may be accomplished by mechanical methods, chemical and enzymatic methods, or a combination of them [46,47]. Based on the current literature, there is no extraction method that is 100% effective in yielding oils derived from microorganisms.…”