Unconventional meiotic process of spermatocytes in male Cyprinus carpio

Zhu, La; He, Wangchao; Zhang, Hui; Sun, Yu; Li, Qi; Zhou, Li; Zhu, Shurun; Tao, Min; Zhou, Yi; Zhao, Rurong; Luo, Kun; Tang, Chenchen; Zhang, Chun; Liu, Shaojun

doi:10.1016/j.repbre.2021.03.001

Cited by 4 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, global information about the transcriptional activities in polyploid fishes is limited. In addition, the mechanisms of spermatogenesis in different ploidy fishes may differ, presenting the key processes to generate haploid and diploid gametes (Zhu et al, 2021). In this study, the 3.…”

Section: Discussionmentioning

confidence: 91%

Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂)

et al. 2021

Self Cite

View full text Add to dashboard Cite

Polyploidy occurs naturally in fish; however, the appearance of these species is an occasional and gradual process, which makes it difficult to trace the changes in phenotypes, genotypes, and regulation of gene expression. The allotetraploid hybrids (4nAT) of red crucian carp (RCC; ♀) × common carp (CC; ♂) generated from interspecies crossing are a good model to investigate the initial changes after allopolyploidization. In the present study, we focused on the changes in the active sites of the testicular transcriptome of the allotetraploid by localization of RNA Pol II CTD YSPTSPS (phospho S5) using immunofluorescence and RNA-seq data via bioinformatic analysis. The results showed that there was no significant difference in signal counts of the RNA Pol II CTD (S5) between the different types of fish at the same stages, including RCC, CC, 2nF1, and 4nAT, which means that the number of transcriptionally active sites on germ cell chromosomes was not affected by the increase in chromosome number. Similarly, RNA-seq analysis indicated that in the levels of chromosomes and 10-kb regions in the genome, there were no significant changes in the highly active sites in RCC, 2nF1, and 4nAT. These findings suggest that at the beginning of tetraploid origin, the active transcriptome site of 4nAT in the testis was conserved in the regions of the genome compared to that in RCC and 2nF1. In conclusion, 4nAT shared a similar gene expression model in the regions of the genome with RCC and 2nF1 with significantly different expression levels.

show abstract

Section: Discussionmentioning

confidence: 91%

Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂)

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, the morphology and expression of meiotic marker genes of germ cell-like cells directly differentiated from Kio-CiPSLCs were similar to those of sperm-like cells induced in fish and mammals ( Hong et al, 2004 ; Kawasaki et al, 2016 ; Wang et al, 2016 ; Zhou et al, 2016 ). During the spermatogenesis of common carp ( Cyprinus carpio ), the secondary spermatocytes also formed the typical circular condensed nucleus and spermatids were further packed with characteristic of higher nucleo-cytoplasmic ratios ( Zhu et al, 2021 ). Compared with the real iPSCs, the chemically induced pluripotent stem cell-like cells we induced can produce most of the cell types of adult fish but have not been able to produce functional gametes.…”

Section: Discussionmentioning

confidence: 99%

Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality

Li²,

Peng³

et al. 2022

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

The technique of induced pluripotent stem cells has significant application value in breeding and preserving the genetic integrity of fish species. However, it is still unclear whether the chemically induced pluripotent stem cells can be induced from non-mammalian cells or not. In this article, we first verify that fibroblasts of fish can be chemically reprogrammed into pluripotent stem cells. These induced pluripotent stem-like cells possess features of colony morphology, expression of pluripotent marker genes, formation of embryoid bodies, teratoma formation, and the potential to differentiate into germ cell-like cells in vitro. Our findings will offer a new way to generate induced pluripotent stem cells in teleost fish and a unique opportunity to breed commercial fish and even save endangered fish species.

show abstract

“…The typical fish spermatozoa consist of a primitive head without acrosome, a midpiece with several mitochondria, a centriolar complex (proximal and distal centriole), and one flagellum ( Psenicka et al, 2009 ; Zhu et al, 2021 ). The basic task of the spermatozoon head is to transfer the genetic material localized in the nucleoplasma to the egg.…”

Section: Discussionmentioning

confidence: 99%

Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)

Sun

Qiang

et al. 2021

Front. Genet.

Self Cite

View full text Add to dashboard Cite

The spermatozoa of triploid gynogenetic crucian carp (Carassius auratus) (3nDTCC) possess a spermatogenesis process with a normal genetic background. However, the genetic materials of these spermatozoa do not completely inherit gynogenetic progeny in general. Understanding the intrinsic mechanism may be helpful for developing breeding strategies of gynogenetic fishes. In this study, the spermatozoa ultrastructure was systematically studied in diploid red crucian carp and 3nDTCC to demonstrate their cytological structural differences. In addition, the artificial breeding tests of 3nDTCC(♀) with different ploidy spermatozoa were performed to verify the contributions of genetic materials from 3nDTCC spermatozoa to the gynogenesis progeny. Furthermore, the mRNA expression of centriole-related genes (i.e., cep57, cetn1, rootletin, and nek2) involved in spermatozoa packaging was also determined by quantitative real-time PCR (qPCR) to illustrate the molecular expression characteristics of the spermatozoa packaging process in 3nDTCC. The results reveal the adaptive features of spermatozoa in 3nDTCC, including the loose midpiece structure, abnormal head structure, and abnormal expression of centriole-related genes, which may influence the motility of spermatozoa and make it not involved normally in the genetic composition of the gynogenesis offspring.

show abstract

Unconventional meiotic process of spermatocytes in male Cyprinus carpio

Cited by 4 publications

References 17 publications

Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂)

Localization of RNA Pol II CTD (S5) and Transcriptome Analysis of Testis in Diploid and Tetraploid Hybrids of Red Crucian Carp (♀) × Common Carp (♂)

Fish Pluripotent Stem-Like Cell Line Induced by Small-Molecule Compounds From Caudal Fin and its Developmental Potentiality

Structural Abnormalities of Spermatozoa in Triploid Gynogenetic Crucian Carp (Carassius auratus)

Contact Info

Product

Resources

About