2005
DOI: 10.1128/jb.187.22.7569-7578.2005
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Unconventional Mode of Attachment of the Ruminococcus flavefaciens Cellulosome to the Cell Surface

Abstract: Sequence extension of the scaffoldin gene cluster from Ruminococcus flavefaciens revealed a new gene (scaE) that encodes a protein with an N-terminal cohesin domain and a C terminus with a typical gram-positive anchoring signal for sortase-mediated attachment to the bacterial cell wall. The recombinant cohesin of ScaE was recovered after expression in Escherichia coli and was shown to bind to the C-terminal domain of the cellulosomal structural protein ScaB, as well as to three unknown polypeptides derived fro… Show more

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Cited by 89 publications
(122 citation statements)
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“…As shown in Fig. 1A, the overall organization of the scaffoldin cluster of strain FD-1 matches well with that of strain 17 (17). The cluster consists of genes encoding four scaffoldins of different sizes and one additional gene, cttA, encoding a protein of currently unknown function.…”
mentioning
confidence: 52%
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“…As shown in Fig. 1A, the overall organization of the scaffoldin cluster of strain FD-1 matches well with that of strain 17 (17). The cluster consists of genes encoding four scaffoldins of different sizes and one additional gene, cttA, encoding a protein of currently unknown function.…”
mentioning
confidence: 52%
“…Both scaffoldins contain a characteristic X-module/dockerin pair (XDoc) at the C terminus. The integrity of the strain 17 ScaB XDoc has recently been shown to be critical for recognition by the ScaE cohesin (17). The differences between the two ScaB scaffoldins, however, are not only quantitative (i.e., in the respective number of cohesins) but also qualitative.…”
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confidence: 91%
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“…Although acetate takes a major part in SCFA, important actions of butyrate include inhibiting the activities of NF-kb and HDAC, and having a strong anti-tumorigenic function. When treated with butyrate, with the help of the Bcl-2 family (which comprises pro-apoptotic protein and anti-apoptotic protein), cytochrome c in mitochondria comes out to cytoplasm and activates caspase so as to induce apoptosis, and also coordinates the TNF receptor super-family and inhibits the activities of HDAC, which is involved in gene expression, so as to induce apoptosis [2,51,66,69]. Relying or not relying on p53, butyrate induces cell cycle arrest and apoptosis.…”
Section: Discussionmentioning
confidence: 99%