Uncoupling effect of nitrite during denitrification byPseudomonas fluorescens: An in vivo31P-NMR study

Sijbesma, Wilbert F. H.; Almeida, Jonas S.; Reis, Maria A.M.; Santos, Helena

doi:10.1002/(sici)1097-0290(19961005)52:1<176::aid-bit18>3.0.co;2-m

Cited by 69 publications

(14 citation statements)

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“…It is known to inhibit photosynthetic electron transport (Spiller and Boger 1977;Loranger and Carpentier 1994), change intracellular pH and damage cell membranes of (Almeida et al 1995;Sijbesma et al 1996;Yang et al 2004). In the present study, a significant increase was observed in intracellular nitrite concentrations under high nitrate concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Sijbesma et al (1996) has reported that nitrite ion acted as a protonophore, an uncoupler that increases the proton permeability of membranes by a shunting mechanism, inhibiting ATP synthesis and stimulating ATP hydrolysis. Almeida et al (1995) proposed that nitrite could induce a lower efficiency of respiratory-chain-linked energy conservation, due to an increase of proton permeability by the nitrite ion, counteracting the proton pumping effect of ATP-ase.…”

Section: Introductionmentioning

confidence: 99%

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Effects of nitrate on intracellular nitrite and growth of Microcystis aeruginosa

2009

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Although nitrate is a macronutrient and can serve as good nitrogen source for many species of phytoplankton, high nitrate concentrations do not benefit the growth of phytoplankton. We hypothesise that algae cultured under high nitrate concentrations can accumulate intracellular nitrite, which is produced by nitrate reductase (NR) and can inhibit the growth of algae. To assess the validity of this hypothesis, Microcystis aeruginosa was grown under different nitrate concentrations from 3.57 to 21.43 mM in low CO 2 and high CO 2 conditions for 15 days. We observed that, with increasing nitrate concentrations, the intracellular nitrite concentrations of the alga increased and the growth rates and photosynthesis declined. When grown under high CO 2 conditions, M. aeruginosa showed lower intracellular nitrite concentrations and higher growth rates and P chla m , R chla d , α chla than under low CO 2 conditions. These results suggest that the accumulation of intracellular nitrite could be the cause of inhibition of algal growth under high nitrate concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of nitrate on intracellular nitrite and growth of Microcystis aeruginosa

2009

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“…The bacteria in the mixed sludge show different cellular responses to chemical stress, including FNA shock. Polyphosphate accumulating organisms (PAOs) are inhibited by FNA in concentrations of as low as 10 μg L −1 , 31 whereas some FNA-tolerant bacteria survive and show high resistance to hydrolysis, 24 resulting in low sludge disintegration rates during FNA treatment. In this study, Percoll-purified anammox granules, which can utilize a high concentration of nitrite, were selected for analyzing the transmembrane ionic currents under low-concentration FNA shock.…”

Section: Methodsmentioning

confidence: 99%

“…22,23 The unique properties of FNA treatment on WAS disposal have been demonstrated; however, the inhibition mechanism and cellular response toward low-concentration FNA remain inconclusive. Sijbesma et al 24 have found that FNA can act as a protonophore to inhibit the ATP synthesis of Pseudomonas uorescens. Park 25 discovered that FNA could react directly with sulydryl-containing enzymes and affect the tricarboxylic acid (TCA) cycle.…”

Section: Introductionmentioning

confidence: 99%

Performance and mechanism of free nitrous acid on the solubilization of waste activated sludge

Wang

Zhang

et al. 2018

RSC Adv.

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“…Therefore, to maintain the balance of intracellular and intracellular potentials, cells need to expel excess free protons from the intracellular to the extracellular. This process will consume additional ATP, resulting in insufficient ATP to maintain normal metabolism of cells and inhibit cell survival ( Anthonisen et al, 1976 ; Sijbesma et al, 1996 ; Gao et al, 2016 ). However, Vadivelu et al (2006a) found that FNA does not inhibit the ATP production and metabolism of all microorganisms, and the uncoupling effect of FNA alone may not be the only mechanism for FNA inhibition ( Vadivelu et al, 2006a , b ).…”

Section: Function Of Fna On Pn Establishment: Bactericidal Effect At ...mentioning

confidence: 99%

Achieving Partial Nitritation by Treating Sludge With Free Nitrous Acid: The Potential Role of Quorum Sensing

Jiang

Wang

et al. 2022

Front. Microbiol.

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Partial nitritation is increasingly regarded as a promising biological nitrogen removal process owing to lower energy consumption and better nitrogen removal performance compared to the traditional nitrification process, especially for the treatment of low carbon wastewater. Regulating microbial community structure and function in sewage treatment systems, which are mainly determined by quorum sensing (QS), by free nitrous acid (FNA) to establish a partial nitritation process is an efficient and stable method. Plenty of research papers reported that QS systems ubiquitously existed in ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), and various novel nitrogen removal processes based on partial nitritation were successfully established using FNA. Although the probability that partial nitritation process might be achieved by the regulation of FNA on microbial community structure and function through the QS system was widely recognized and discussed, the potential role of QS in partial nitritation achievement by FNA and the regulation mechanism of FNA on QS system have not been reviewed. This article systematically reviewed the potential role of QS in the establishment of partial nitritation using FNA to regulate activated sludge flora based on the summary and analysis of the published literature for the first time, and future research directions were also proposed.

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Uncoupling effect of nitrite during denitrification byPseudomonas fluorescens: An in vivo31P-NMR study

Cited by 69 publications

References 20 publications

Effects of nitrate on intracellular nitrite and growth of Microcystis aeruginosa

Effects of nitrate on intracellular nitrite and growth of Microcystis aeruginosa

Performance and mechanism of free nitrous acid on the solubilization of waste activated sludge

Achieving Partial Nitritation by Treating Sludge With Free Nitrous Acid: The Potential Role of Quorum Sensing

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