Uncoupling protein-3: a muscle-specific gene upregulated by leptin in ob/ob mice

Liu, Qingyun; Bai, Chang; Chen, Fang; Wang, Ruiping; MacDonald, Terry; Gu, Mingcheng; Zhang, Qing; Morsy, Manal; Caskey, C. Thomas

doi:10.1016/s0378-1119(97)00596-9

Cited by 90 publications

(64 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present data indicate that UCP2, UCP3, StUCP and AtUCP alter the mitochondrial membrane potential recorded in i o in recombinant yeast and mammalian cells, and probably uncouple respiration [17,30,31,57,[59][60][61]84,85]. The respiration of isolated yeast mitochondria into which UCP2 had been introduced was less coupled than that of control mitochondria [30].…”

Section: Proton Transport Activity Of Ucp2 Ucp3 and Plant Ucps And mentioning

confidence: 57%

“…UCP2 was also cloned by others using sequencing of cDNA libraries or reverse transcriptase-PCR analysis [31,55]. A third UCP, referred to as UCP3 and sharing 72 % identity with UCP2, was identified by several groups a few months after the discovery of UCP2 [55][56][57][58][59]. Figure 1 shows the distribution of UCP1, UCP2 and UCP3 mRNAs in mouse tissues.…”

Section: Figure 1 Northern Analysis Of Ucp1 Ucp2 and Ucp3 Mrnas In Mmentioning

confidence: 99%

See 1 more Smart Citation

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Ricquier¹,

Bouillaud

2000

Biochemical Journal

614

115

View full text Add to dashboard Cite

Animal and plant uncoupling protein (UCP) homologues form a subfamily of mitochondrial carriers that are evolutionarily related and possibly derived from a proton\anion transporter ancestor. The brown adipose tissue (BAT) UCP1 has a marked and strongly regulated uncoupling activity, essential to the maintenance of body temperature in small mammals. UCP homologues identified in plants are induced in a cold environment and may be involved in resistance to chilling. The biochemical activities and biological functions of the recently identified mammalian UCP2 and UCP3 are not well known. However, recent data support a role for these UCPs in State 4 respiration, respiration uncoupling and proton leaks in mitochondria. Moreover, genetic studies suggest that UCP2 and UCP3 play a part in

show abstract

Section: Proton Transport Activity Of Ucp2 Ucp3 and Plant Ucps And mentioning

confidence: 57%

Section: Figure 1 Northern Analysis Of Ucp1 Ucp2 and Ucp3 Mrnas In Mmentioning

confidence: 99%

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Ricquier¹,

Bouillaud

2000

Biochemical Journal

614

115

View full text Add to dashboard Cite

show abstract

“…Although several studies have shown that UCP3 has uncoupling activity when expressed in yeast [12,13] or mouse myoblasts [11], the fact that UCP3 expression consistently increases during fasting (i. e. when thermogenesis is decreasing) could indicate a primary physiological role as an effector in the metabolic adaptation to increased fatty acid supply [41±43]. A role in fuel partitioning is supported by the increased respiratory quotient observed in subjects with the exon 6 splice variant [29].…”

Section: Discussionmentioning

confidence: 99%

Evidence that single nucleotide polymorphism in the uncoupling protein 3 (UCP3) gene influences fat distribution in women of European and Asian origin

et al. 2000

View full text Add to dashboard Cite

The rising prevalence of Type II (non-insulin-dependent) diabetes and obesity worldwide poses a serious challenge to human health and incomplete understanding of the aetiological basis of these closely related metabolic conditions is a major impediment to improved management. There is considerable evidence to suggest that primary abnormalities in energy balance contribute to the pathogenesis of diabetes and obesity [1]. Reduced energy expenditure is correlated with subsequent weight gain [2] and normoglycaemic women at increased risk of future diabetes have defective post-prandial thermogenesis [3,4]. Diabetologia (2000) Abstract Aims/hypothesis. Uncoupling proteins are mitochondrial transmembrane carriers implicated in the regulation of energy balance. Dysfunction of UCP3 (the predominant uncoupling protein in skeletal muscle) might therefore be expected to reduce thermogenic capacity, alter energy homeostasis and influence predisposition to obesity and Type II (non-insulin-dependent) diabetes mellitus. A variant in the putative promoter region of UCP3 (±55 c®t) has recently been identified, and an association with obesity reported in French subjects. Our aim was to study the pathophysiological role of this variant in diabetes-related and obesity-related traits using two distinct ethnic populations. Methods. The ±55 c®t variant was genotyped in 85 South Indian and 150 European parent-offspring trios ascertained through Type II diabetic probands and in 455 South Indian subjects initially recruited to an urban survey into the prevalence of diabetes. Results. In South Indian and European parent-offspring trios there was no preferential transmission of either allele at the ±55 c®t polymorphism to diabetic offspring (South Indians, p = 0.60; Europeans, p = 0.15). When family members were analysed for intermediate traits, the t-allele was associated with increased waist-to-hip ratio but only in females (South Indian mothers p = 0.036, daughters p = 0.032: European mothers p = 0.037, daughters p = 0.14). These findings were replicated in South Indian females from the population-based survey (p = 0.039). Conclusion/interpretation. The consistent association between the t-allele at this locus and increased waist-to-hip ratio in women from three separate data sets indicates that variation at this polymorphism (or another locus with which it is in linkage disequilibrium) influences fat distribution but that this effect is restricted to females. [Diabetologia (2000)

show abstract

“…There is a great deal of evidence implicating UCP3 in mitochondrial uncoupling: UCP3, like UCP1, uncouples when expressed in yeast (6,7,(11)(12)(13), mammalian cells (9), or proteoliposomes (8,14). However, there is strong evidence that the uncoupling seen in yeast is an expression artifact (13,(15)(16)(17)(18).…”

mentioning

confidence: 99%

The Basal Proton Conductance of Skeletal Muscle Mitochondria from Transgenic Mice Overexpressing or Lacking Uncoupling Protein-3

Cadenas¹,

Echtay²,

Harper³

et al. 2002

Journal of Biological Chemistry

189

167

View full text Add to dashboard Cite

The ability of native uncoupling protein-3 (UCP3) to uncouple mitochondrial oxidative phosphorylation is controversial. We measured the expression level of UCP3 and the proton conductance of skeletal muscle mitochondria isolated from transgenic mice overexpressing human UCP3 (UCP3-tg) and from UCP3 knockout (UCP3-KO) mice. The concentration of UCP3 in UCP3-tg mitochondria was ϳ3 g/mg protein, ϳ20-fold higher than the wild type value. UCP3-tg mitochondria had increased nonphosphorylating respiration rates, decreased respiratory control, and ϳ4-fold increased proton conductance compared with the wild type. However, this increased uncoupling in UCP3-tg mitochondria was not caused by native function of UCP3 because it was not proportional to the increase in UCP3 concentration and was neither activated by superoxide nor inhibited by GDP. UCP3 was undetectable in mitochondria from UCP3-KO mice. Nevertheless, UCP3-KO mitochondria had unchanged respiration rates, respiratory control ratios, and proton conductance compared with the wild type under a variety of assay conditions. We conclude that uncoupling in UCP3-tg mice is an artifact of transgenic expression, and that UCP3 does not catalyze the basal proton conductance of skeletal muscle mitochondria in the absence of activators such as superoxide.

show abstract

Uncoupling protein-3: a muscle-specific gene upregulated by leptin in ob/ob mice

Cited by 90 publications

References 25 publications

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP

Evidence that single nucleotide polymorphism in the uncoupling protein 3 (UCP3) gene influences fat distribution in women of European and Asian origin

The Basal Proton Conductance of Skeletal Muscle Mitochondria from Transgenic Mice Overexpressing or Lacking Uncoupling Protein-3

Contact Info

Product

Resources

About